Rice hybrid breeding material as well as cultivation method and application thereof

A rice hybridization and breeding method technology, applied in the field of rice seed production, can solve the problems of cumbersome steps, difficulty in ensuring purity, and high seed production costs, and achieve the effects of simple reproduction, improved purity, and easy control

Inactive Publication Date: 2013-07-31
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to overcome the deficiencies of the prior art, the purpose of the present invention is to provide a rice hybrid breeding material and its cultivati...

Method used

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  • Rice hybrid breeding material as well as cultivation method and application thereof
  • Rice hybrid breeding material as well as cultivation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 The cultivation method of rice hybrid breeding material

[0030] Take rice varieties NS629S and RH003 as examples;

[0031] (1) Cultivation method of female parent material

[0032] Clone the stamen-specific promoter of Osg6B and drive the antisense fragment of the cyhalofop-methyl-sensitive gene or bentazone-sensitive gene to transform the rice variety NS629S. In the transgenic progeny, a single-copy insertion was selected, the agronomic traits were good, and only in the stamen part. Plants with high sensitivity to cyhalofop or bentazone were used as female parent material;

[0033] (2) Cultivation method of male parent material

[0034] Clone the pistil-specific promoter of STS14 to drive the antisense fragment of cyhalofop-methyl-sensitive gene or bentazone-sensitive gene and introduce it into rice variety RH003. In the transgenic progeny, a single copy insertion was selected, the agronomic traits were excellent, and only in the pistil part was cyanide...

Embodiment 2

[0035] Embodiment 2, mechanized mixed sowing seed production (plantation in Hefei, Anhui)

[0036] (1) Mix the seeds of the parent materials obtained from the invention with a mass ratio of 1:6 (male parent: female parent), sow seeds from May 10th to May 15th, and use conventional water seedling breeding methods to cultivate strong seedlings. The rice transplanting adopts Kubota SPW-48C automatic rice transplanter, with an average row spacing of 30 cm and a plant spacing of 18 cm. Field planting adopts conventional water and fertilizer management, and sprays corresponding herbicides (such as cyhalofop-ester, water agent mass fraction 0.25%, spraying 50L-60L per mu) about 7 days before heading, kill males (female parent), Female (male parent); seeds harvested by harvester. After sampling inspection, the tested seeds were all hybrids, and no parents were self-bred and mixed.

[0037] According to the type of herbicide, control the concentration, time and location of sprayi...

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Abstract

The invention discloses a rice hybrid breeding material as well as a cultivation method and application thereof. A female parent material with a herbicide sensitive gene and a male parent material with the herbicide sensitive gene are obtained by specific promoter drive and antisense inhibit expression methods; the herbicide sensitive gene is just expressed in stamen; and the herbicide sensitive gene is just expressed in pistil; the female parent material and the male parent material are mixed in application; mechanized cropping is carried out; the female parent emasculation and male parent female removal are achieved by spraying the herbicide before heading; and the high-quality F1 hybrid seed is produced by one-way mating between parents. By adopting the method disclosed by the invention, a sterile line and a restorer line can be freely obtained; high-purity hybrid is obtained; mixing of the self-fruitfulness seed is effectively removed; especially, serious decline of hybrid purity caused by fertility recovery of the photo-thermo-sensitive sterile line under an adverse light temperature condition is avoided; the breeding process of the parents of the hybrid rice is simplified; manpower and material resources are saved; and the production efficiency is improved.

Description

technical field [0001] The invention relates to the field of rice seed production, in particular to a rice hybrid breeding material and its cultivation method and application. Background technique [0002] Hybrid rice has made great contributions to food security in my country and the world. However, in the existing rice heterosis utilization and seed production, the combination is not free, and the seed production links are many, the process is complicated, too much depends on intensive manpower and material resources, and the production efficiency is low. Especially in recent years, with the development of my country's economy, young and middle-aged people in rural areas have migrated to cities to work, and there is a shortage of rural labor force. The production of hybrid rice seeds is facing serious challenges. The realization of hybrid rice mechanized mixed seed production is of great significance to reduce the cost of hybrid rice seed production, improve the quality a...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 杨剑波魏鹏程宋丰顺倪金龙倪大虎李浩李泽福陆徐忠
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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