Rice hybrid breeding material as well as cultivation method and application thereof
A breeding method and rice hybridization technology, which is applied in the field of rice seed production, can solve the problems of difficulty in ensuring purity, cumbersome steps, and high cost of seed production, and achieve the effects of improving purity, simple reproduction, and easy control
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Embodiment 1
[0029] Embodiment 1 The cultivation method of rice hybrid breeding material
[0030] Take rice varieties NS629S and RH003 as examples;
[0031] (1) Cultivation method of female parent material
[0032] Clone the stamen-specific promoter of Osg6B and drive the antisense fragment of the cyhalofop-methyl-sensitive gene or bentazone-sensitive gene to transform the rice variety NS629S. In the transgenic progeny, a single-copy insertion was selected, the agronomic traits were good, and only in the stamen part. Plants with high sensitivity to cyhalofop or bentazone were used as female parent material;
[0033] (2) Cultivation method of male parent material
[0034] Clone the pistil-specific promoter of STS14 to drive the antisense fragment of cyhalofop-methyl-sensitive gene or bentazone-sensitive gene and introduce it into rice variety RH003. In the transgenic progeny, a single-copy insertion is selected, the agronomic traits are good, and only in the pistil part of the cyanide Pl...
Embodiment 2
[0035] Embodiment 2, mechanized mixed sowing seed production (plantation in Hefei, Anhui)
[0036] (1) Mix the seeds of the parent material obtained from the invention with a mass ratio of 1:6 (male parent: female parent), sow seeds from May 10th to May 15th, and use conventional water seedling breeding methods to cultivate strong seedlings. The rice transplanting adopts Kubota SPW-48C automatic rice transplanter, with an average row spacing of 30 cm and a plant spacing of 18 cm. Field planting adopts conventional water and fertilizer management, and sprays corresponding herbicides (such as cyhalofop-ethyl, water agent mass fraction 0.25%, spraying 50L-60L per mu) about 7 days before heading, kill males (female parent), Female (male parent); seeds harvested by harvester. After sampling inspection, the tested seeds were all hybrids, and no parents were self-bred and mixed.
[0037] According to the type of herbicide, control the concentration, time and location of sprayin...
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