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Transgene crop pollen competitive agent and method for cotten seed preparation

A competitive agent and transgenic technology, applied in botany equipment and methods, plant gene improvement, application, etc., can solve the problems of restricting the large-scale utilization of the first-generation hybrid seeds, the inability to guarantee the purity of hybrids, and the inapplicability of hybrids, so as to solve the problem of direct pollination Difficulties, saving the cost of artificial seed production, and the effect of labor-saving seed production

Inactive Publication Date: 2006-08-30
朱安灿
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this production method is that it requires a lot of labor, the seeds are miscellaneous, and the price is high, which limits the large-scale utilization of the first-generation hybrid seeds.
However, because the proportion of false hybrids produced by self-pollination at the same time is as high as more than 50%, the purity of hybrids cannot be guaranteed, so this method has not been applied in seed production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Anti-glyphosate, insect-resistant cotton hybrid combination, use kanamycin, glyphosate and sucrose in distilled water (the same as the following examples) to prepare pollen competitors, wherein:

[0043] Kanamycin 0.08g·L -1 , sucrose 460g·L -1 ,

[0044] Glyphosate 0.042g·L -1 .

[0045] Add 0.08 g of kanamycin, 460 g of sucrose, and 0.042 g of glyphosate into 1000 ml of distilled water, and then add 0.1 g of Tween 80 as a spreading agent.

[0046] The non-transgenic cotton E disease-resistant cotton B was used as the female parent, and the glyphosate-resistant cotton A was used as the male parent to form a hybrid combination. Every morning during the flowering period, the seed producer holds a small sprayer with a pollen competitor in one hand, sprays the stigma and anthers of the female parent B, and then takes an open flower of the male parent A with the other hand to pollinate the stigma of the male parent B. Hybridization; one male flower can pollinate 5 fema...

Embodiment 2

[0051] A kanamycin-resistant insect-resistant cotton hybrid combination, kanamycin and sucrose are formulated with distilled water as a pollen competitor, wherein:

[0052] Kanamycin 0, 1g·L -1 , sucrose 420g·L -1 .

[0053] Add 0.01% Tuwen 80 as spreading agent when spraying.

[0054] The non-transgenic cotton E disease-resistant cotton B was used as the female parent, and the transgenic insect-resistant cotton A was used as the male parent to form a high-yielding combination. In the morning, take 30 grams of pollen from the male parent and put it into 100 milliliters of pollen competition solution, dip the pollen solution with a brush, apply it on the stigma of the female parent flower, or spray the stigma with a sprayer, and complete the hybrid pollination of a flower.

[0055] After sowing and emergence of hybrid seeds, use 5g·L -1 The seedlings were sprayed with kanamycin solution, and after 10 days, the pseudohybrid seedlings that were not resistant to the macula wer...

Embodiment 3

[0057] Insect-resistant cotton combination with dominant marker traits such as chicken feet, leaves, and no glands, neomycin, sucrose, gibberellin, boric acid, calcium nitrate, and magnesium sulfate contain in the pollen competitor solution:

[0058] Neomycin 0.07g·L -1 , sucrose 450g·L -1 , gibberellin 0, 1g·L -1 ,

[0059] Boric acid 0, 1g·L -1 , calcium nitrate 0, 3g·L -1 , magnesium sulfate 0, 2g·L -1 .

[0060] The method of using the pollen competitor is the same as in Example 2. Its method of selecting true and false hybrids is the same as that of embodiment 2, except that false hybrids that do not have chicken foot leaves and no gland markers can be further pulled out. The purity of hybrid seeds reaches 98%.

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Abstract

An application of the transgenic crop's pollen competitor in culturing the hybrid cotton includes such steps as choosing the non-resistant cotton as female parent, choosing transgenic cotton as male parent, spraying the pollen competitor onto the flower of female parent and pollinating with the pollen of male parent or mixing the pollen of male parent with the pollen competitor liquid and spraying the mixture onto the flower of female parent, hybridizing, and coating the obtained seeds with the seed or seedling purity selecting agent.

Description

technical field [0001] The invention relates to a transgenic pollen competitor for transgenic crop hybrid seed production and a method for using the competitor to perform hybrid seed production on transgenic crops. In particular, a transgenic pollen competitor and seed and seedling purity selection agent for hybrid seed production, and a method for hybrid seed production of transgenic cotton using the pollen competitor and seed and seedling purity selection agent. The invention belongs to the technical field of agricultural hybrid seed production. Background technique [0002] At present in my country, the production methods of hybrid seeds of transgenic crops (such as cotton) mainly rely on artificial detasseling and artificial pollination. The disadvantage of this production method is that it takes a lot of labor, the seeds are miscellaneous, and the price is high, which limits the large-area utilization of the first-generation hybrid seeds. After the combination of high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 朱安灿朱昊
Owner 朱安灿
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