Molecular marker in close linkage with rape crotch angle character QTL (Quantitative Trait Loci) and application

A branching angle and molecular marker technology, applied in the field of molecular biology and genetic breeding, can solve problems such as heavy workload, long process cycle, and constraints on the progress of QTL positioning, and achieve accurate and rapid screening, saving production costs, and convenient detection methods quick effect
CN105969852AActive Publication Date: 2016-09-28INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Publication Date
2016-09-28

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Abstract

The invention discloses a molecular marker in close linkage with rape crotch angle character QTL (Quantitative Trait Loci) and application. The molecular marker is characterized by carrying out hybridization by taking cabbage type rape variety Shanghai oil 19 as a female parent and Purler as a male parent, establishing F2 segregation population through selfing of hybrid F1, and analyzing, thus obtaining a crotch angle locus qBA1.A06; designing a primer by utilizing an Indel marker at the boundary of the crotch angle locus qBA1.A06 so as to detect the parents and the F2 population, thus obtaining a molecular marker BAIndel76 and a molecular marker BAIdenl79 which are in close linkage with the crotch angle character QTL; identifying a rape gene type formed after hybridization of the two parents by utilizing a marker primer, and carrying out auxiliary selection by utilizing the marker, thus greatly increasing the selection efficiency. According to the molecular marker disclosed by the invention, a novel genetic marker is provided for molecular breeding of the rape plant type, and useful information is also provided for accurate positioning on the crotch angle character QTL of the cabbage type rape and map-based cloning on related genes.
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Description

technical field

[0001] The invention belongs to the technical field of molecular biology and genetic breeding, and more specifically relates to a molecular marker closely linked to a QTL locus for the branch angle trait of rapeseed and its application. Background technique

[0002] Rapeseed is the largest oil crop in my country, and its planting area and output rank among the top in the world. The oil production of rapeseed accounts for 55% of the entire domestic oil crops, and occupies a major position in the supply of domestic vegetable oil. Therefore, increasing the planting area of ​​rapeseed and increasing the yield of rapeseed are of great significance to ensure the security of edible vegetable oil supply in my country (Wang Hanzhong, et al. 2014). However, with the continuous rise of labor prices and production costs, the production efficiency of rapeseed has been significantly reduced, which has led to stagnant or even declining rapeseed acreage in recent years. Poo...

Claims

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