51results about How to "Selection inefficiency" patented technology

The invention discloses a molecular marker in close linkage with rape crotch angle character QTL (Quantitative Trait Loci) and application. The molecular marker is characterized by carrying out hybridization by taking cabbage type rape variety Shanghai oil 19 as a female parent and Purler as a male parent, establishing F2 segregation population through selfing of hybrid F1, and analyzing, thus obtaining a crotch angle locus qBA1.A06; designing a primer by utilizing an Indel marker at the boundary of the crotch angle locus qBA1.A06 so as to detect the parents and the F2 population, thus obtaining a molecular marker BAIndel76 and a molecular marker BAIdenl79 which are in close linkage with the crotch angle character QTL; identifying a rape gene type formed after hybridization of the two parents by utilizing a marker primer, and carrying out auxiliary selection by utilizing the marker, thus greatly increasing the selection efficiency. According to the molecular marker disclosed by the invention, a novel genetic marker is provided for molecular breeding of the rape plant type, and useful information is also provided for accurate positioning on the crotch angle character QTL of the cabbage type rape and map-based cloning on related genes.

The invention relates to the technical field of molecular markers, and particularly relates to an SNP molecular marker related to pod and seed size on a peanut A06 chromosome and application thereof.The SNP molecular marker related to the pod and seed size on the peanut A06 chromosome is A06-107901527, and contains a nucleotide sequence with G/T polymorphism at the 12 site of the sequence shown in SEQ ID NO. 1. In the SNP molecular marker A06-107901527, the genotype of the site with the polymorphism is GG, the character value corresponding to the peanut pod and seed size is high, the genotype is TT, and the character value corresponding to the peanut pod and seed size is low. The SNP molecular marker is used for identifying the character phenotype of the peanut pod and seed size, theproduction cost can be greatly saved, and the selection efficiency can be improved.

The invention discloses a rapeseed pod number major QTL molecular marker and its application. The marker can be used for marker-assisted selection in pod number character improvement and fine mapping and map-based cloning of QTL site. Primers of the molecular marker are CNU400F:5'-CGAGTTTTTGTGTGTACGTATAGTAAT-3' and CNU400R:5'-CCAAAGTGCGTAAAGGAAGG-3'. Rapeseed Zhongshuang 11 and 73290F3 generation are selected by the use of the marker, and silique number of the screened F3 single plant is higher than that of Zhongshuang 11 by 92.3%. Thus, the marker is utilized for assistant selection so as to greatly enhance selection efficiency of high yield breeding.

The invention discloses a molecular marker of a pot shattering resistance trait major gene locus of rapes and an application. The steps are as follows: 1) hybridizing double 11 variety and 73290 variety (which have obvious differences in the pot shattering resistance traits) in a cabbage type rape varieties, and carrying out selfing on progenies to obtain F2 and F2:3 segregation populations with the segregated pod shattering resistance traits; 2) carrying out polymorphic screening on parent DNA (deoxyribonucleic acid) by utilizing an SSR (simple sequence repeat) primer, and establishing a genetic linkage map by carrying out SSR molecular marker genotyping on the F2 progeny segregation populations; 3) carrying out field experiments and plant inquisition on the F2 and F2:3 segregation populations so as to obtain phenotype data of the pod shattering resistance traits; and 4) carrying out QTL (quantitative trait locus) detection by combining with the developed high-density molecular marker genetic linkage map and genotype and the phenotype data of the segregation populations, utilizing QTL Cart2.5 software to obtain the major gene locus Psr.A9 for controlling the pod shattering resistance of the rapes on a cabbage type rape A9 linkage group and also obtain the molecular marker BrSF0007-39 closely linked with the major gene locus. The selection efficiency of breeding with the pod shattering resistance can be greatly improved by utilizing the marker for assistant selection.

A simple method for screening the wheat genotype able to generate unreduced gamete for breaking through the 'reduplicative bottle neck' of reduplicating monoploid includes such steps as distant hybridizing between wheat and Aegilops variabilis to obtain plant F1, selfing, and screening.

The invention discloses a soybean mosaic virus major gene locus and application thereof, and in particular relates to the soybean mosaic virus major gene locus and the application of the soybean mosaic virus major gene locus in molecule marker assistant selective breeding. The soybean mosaic virus major gene locus is qRSC.13-1 with a contribution rate of 32.4%; the molecule which is tightly interlocked with the mosaic virus major gene locus is marked as GmSSR13-23. The tightly interlocked molecule marker is utilized to detect whether the breeding population system contains the major gene locus or not, so that the mosaic virus performance can be predicated, and the selection rate of the soybean mosaic virus breeding is greatly improved.

The invention discloses a soybean anti-rust gene locus and application thereof, particularly a soybean anti-rust gene locus and application thereof in molecular marker assisted selective breeding. The anti-rust gene locus is rpp.18-1, and the molecular marker closely linked with the anti-rust gene locus is GmSSR18-21. The soybean rust resistance can be predicted by judging whether the closely linked molecular marker detection breeding material contains the major gene locus, thereby greatly enhancing the selection efficiency for soybean anti-rust breeding.

The invention relates to the technical field of molecular breeding, particularly Gossypium-barbadense-Hai-1-derived molecular markers related to fiber Micronaire value and application thereof. The molecular markers are BNL3145330 and HAU0764220. The molecular markers are beneficial to overcoming the defects in the fiber quality identification in the existing breeding technique, and can enhance the fiber Micronaire value selection efficiency and accelerate the culturing progress of high-quality new species.

The invention belongs to the technical fields of molecular biology and genetic breeding and particularly discloses molecular marker primers of major gene loci of an NSS (Number of Seeds per Silique) trait of rape and application of the molecular marker primers. Phenotype data of the NSS trait are obtained through subjecting F2 and F2 :3 family separated colonies of Zhongshuang 11# and 73290 to field experiment and seed examination; QTL (Quantitative Trait Locus) detection is carried out with reference to genotypes and genetic maps of an F2 separated colony. The major gene loci, i.e., qSN.A7 and a molecular marker Ni201 for controlling NSS of the rape on an A7 linkage group are obtained. Through subjecting F3 derived from two parent plants to genotype analysis by the marker, a result indicates that an NSS mean of selected single plants carrying over favorable genes exceeds that of single plants carrying over unfavorable genes; proven by seed examination results, a mean of NSS of singleplants carrying over favorable markers is higher than that of single plant colonies carrying over the unfavorable genes by 88.0%; therefore, the efficiency of selection of high-yield seed breeding canbe increased greatly through carrying out auxiliary selection by using the marker.

The invention belongs to the technical field of molecular biology and genetic breeding, and particularly discloses a molecular marker CNU288 primer of a rape grain weight trait main effect gene locusand an application. The applicant obtains phenotype data of grain weight traits through performing field experiment and seed selection on F2 genealogy of Zhongshuang No. 11 and 73290; and through combination with genotypes and genetic atlas of F2 segregation populations, QTL detection is performed, and a main effect gene locus qSW.A3 and a molecular marker CNU288 for controlling rape grain weighton an A3 linkage group are obtained. The marker is used for performing genotype analysis on F3 generation derived from two parents, the thousand-grain weight average value (4.89g) of selected single plants carrying favorable genes exceeds the thousand-grain weight average value (4.51g) of single plants carrying unfavorable genes, and the seed selection result indicates that the proportion that thegrain weight of single plants carrying favorable markers is higher than the average value of the grain weight of single plant groups carrying unfavorable genes is as high as 82.9%, so that when the marker is used for performing auxiliary selection, the selection efficiency of high-yield breeding can be greatly improved.