1results about How to "Selection inefficiency" patented technology

The application discloses a corn southern rust resistance gene function marker, the gene is located on the 6th chromosome of corn, from the main QTL site RppQ6.01 of the southern rust resistance of Zi 319, gene silencing test shows that ZmWRKY66 is the disease resistance gene of RppQ6.01, sequence analysis finds that there is an insertion / deletion of CACTA-TIR transposon in the promoter region of ZmWRKY66, two function markers are developed based on the DNA sequence of the transposon, and are named as CACTA-L and CACTA-R. In the application, the main QTL and gene position of the corn southern rust resistance are clear, the phenotype contribution rate is higher, the southern rust resistance of corn inbred line material can be significantly improved, the development and application of the two function markers make the corn seedling stage ZmWRKY66 gene typing detection convenient and fast, and the application is not affected by the environment, and a feasible technical approach is provided for the molecular assisted breeding of the corn southern rust resistance.