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A Molecular Marker Tightly Linked to the Main Effect QTL of Wheat Plant Height Trait and Its Application

A technology of wheat and DNA molecules, which is applied in the field of molecular biology-level genetic breeding, can solve the problem of single source of dwarf genes, etc., and achieve the effect of convenient and fast detection method, clear position, accurate and fast screening

Active Publication Date: 2021-02-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] my country has also used the dwarf gene of wheat in wheat breeding, but the source of the dwarf gene is relatively single

Method used

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  • A Molecular Marker Tightly Linked to the Main Effect QTL of Wheat Plant Height Trait and Its Application
  • A Molecular Marker Tightly Linked to the Main Effect QTL of Wheat Plant Height Trait and Its Application
  • A Molecular Marker Tightly Linked to the Main Effect QTL of Wheat Plant Height Trait and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, construction of wheat plant height positioning population and segregation population and character determination

[0040] The population used in this example is the RIL population derived from Yumai 8679 and Jing 411 and the F 2 population (F derived from a remaining heterozygous line yj-171 in the RIL population 2 group.

[0041] Both the RIL population and the heterozygous line yj-171 are described in the following literature: Zhai HJ, Feng ZY, Li J, Liu XY, Xiao SH, Ni ZF, Sun QX (2016) QTL Analysis of Spike Morphological Traits and Plant Height in Winter Wheat (TriticμM aestivμM L.) Using a High-Density SNP and SSR-Based Linkage Map. Frontiers in Plant Science 7. doi:10.3389 / fpls.2016.01617. Plant height phenotype data for RIL populations are also available from this literature, yj-171-derived F 2 The phenotype of the population is measured.

[0042] yj-171 derived F 2 The population is F 2 group.

[0043] 1. Extraction of total DNA from leaves ...

Embodiment 2

[0063] Example 2, Application of Molecular Marker SSR-2433 in Dwarf Breeding

[0064] 1. Planting F of Yumai 8679 and Jing 411 in the field 2 group.

[0065] 2. Sampling at the seedling stage, and extracting the total DNA of the leaves, and performing PCR amplification on it using the molecular marker SSR-2433. The PCR amplification system is as shown in Table 1, and the PCR amplification reaction is as shown in Table 2.

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Abstract

The invention discloses a molecular marker closely linked with a major QTL of a wheat plant height character, and an application of the molecular marker. The invention provides a primer pair for detecting a to-be-detected wheat plant height, which is a primer pair used for amplifying target fragments; the target fragments are fragments including fragments obtained by amplification through a primer1 and a primer 2; the primer 1 is a single-stranded DNA molecule as shown in a sequence 1; and the primer 2 is a single-stranded DNA molecule shown in a sequence 2. An experiment proves that the primer pair SSR-2433 is used for carrying out assisted selection on wheat segregation populations. A result shows that a single plant with a gene type consistent with that of a high-straw parent Jing 411is obviously higher than a single plant with a gene type consistent with that of a dwarf parent, which indicates that the primer pair SSR-2433 is feasible and effective for molecular marker-assisted selection of wheat dwarf breeding.

Description

technical field [0001] The invention belongs to the technical field of molecular biology-level genetic breeding, and in particular relates to a molecular marker closely linked with the main effect QTL of wheat plant height traits and an application thereof. Background technique [0002] Wheat is the third largest food crop in my country. As the population continues to increase and the area of ​​cultivated land decreases, it is of great practical significance to further increase the per unit area yield of wheat in my country to ensure national food security. The discovery and utilization of key genes related to yield plays an important role in tapping the genetic potential of wheat yield and improving wheat yield per unit area. For example, the breeding of semi-dwarf varieties in the "Green Revolution" has greatly increased the yield of crops such as wheat. The cloning and functional studies of the wheat dwarf gene Rht1 show that one or a few genes may lead to a major break...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 倪中福柴岭岭孙其信翟会杰陈朝燕彭惠茹肖世和
Owner CHINA AGRI UNIV
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