Molecular marker CNU288 primer of rape grain weight trait main effect gene locus and application

A technology of CNU288-F and the main effect gene, which is applied to the molecular marker CNU288 primer. The molecular marker is applied in the high-yield breeding of rapeseed, and can solve the problems of difficult rapeseed breeding application, small QTL effect value, and high quality requirements. The detection method is convenient and fast, accurate and fast screening, and the effect of clear position

Active Publication Date: 2020-05-08
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, AFLP markers also have some disadvantages: high cost, complex process, and technical difficulty; most of the markers are dominant markers; there are high requirements for DNA quality and restriction endonuclease quality
At present, there are some reports on the QTL mapping research on rapeseed grain weight, but usually the detected QTL effect value is small and the reproducibility is not good, so it is difficult to apply in rapeseed breeding

Method used

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  • Molecular marker CNU288 primer of rape grain weight trait main effect gene locus and application
  • Molecular marker CNU288 primer of rape grain weight trait main effect gene locus and application
  • Molecular marker CNU288 primer of rape grain weight trait main effect gene locus and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Construction and trait determination of rapeseed grain weight segregation population:

[0027] The segregation populations used in this example are the F2 derived from the parents of large and small rapeseed Zhongshuang 11 and 73290 (planted at the Yangluo Comprehensive Experimental Base of the Institute of Oil Plants, Chinese Academy of Agricultural Sciences in 2009) and its F2: 4 family line (planted in 2011 at the experimental base of Qinghai University). The grain weight phenotypes of the two parents and the two populations were identified after harvesting at maturity. The grain weight test data showed that the two parents had weak superparent segregation, indicating that the large-grain gene was mainly distributed in the genome of Zhongshuang 11; the population grain weight was normally distributed, which proved the quantitative genetic characteristics of the grain weight trait ( figure 1 ).

[0028] F2 inspected a group, the W09F2b group tested the thousand-grai...

Embodiment 2

[0030] Primer development and synthesis:

[0031] The SSR primers used by the applicant include two types: one is the published primer sequence in published articles and Brassica database (http: / / www.br assica.info / resource / markers / ssr-exchange.php); the other is The class was developed by the applicant based on the sequences of cabbage and cabbage scaffolds, named BrSF and BoSF series respectively. The specific development method is to use SSR Hunter software to search for SSRs in each scaffold, and then use Primer3.0 software to design SSR primers. The self-developed InDel primers are obtained by comparing the resequencing sequence of 73290 with the reference genome sequence of Zhongshuang11. First, the resequencing sequence of 73290 is positioned on the whole genome reference sequence of Zhongshuang11 using BWA software, and then Use samtools software to find InDel.

Embodiment 3

[0033] The screening process of primer polymorphism is as follows:

[0034] (1) Randomly select 10 strains of DNA from each parent and mix them in equal amounts as templates for screening primers.

[0035] (2) Utilize the primers after dissolving to carry out PCR amplification to the parental DNA,

[0036] reaction system:

[0037]

[0038] PCR reaction program:

[0039]

[0040]

[0041] (3) Gel electrophoresis band pattern interpretation

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Abstract

The invention belongs to the technical field of molecular biology and genetic breeding, and particularly discloses a molecular marker CNU288 primer of a rape grain weight trait main effect gene locusand an application. The applicant obtains phenotype data of grain weight traits through performing field experiment and seed selection on F2 genealogy of Zhongshuang No. 11 and 73290; and through combination with genotypes and genetic atlas of F2 segregation populations, QTL detection is performed, and a main effect gene locus qSW.A3 and a molecular marker CNU288 for controlling rape grain weighton an A3 linkage group are obtained. The marker is used for performing genotype analysis on F3 generation derived from two parents, the thousand-grain weight average value (4.89g) of selected single plants carrying favorable genes exceeds the thousand-grain weight average value (4.51g) of single plants carrying unfavorable genes, and the seed selection result indicates that the proportion that thegrain weight of single plants carrying favorable markers is higher than the average value of the grain weight of single plant groups carrying unfavorable genes is as high as 82.9%, so that when the marker is used for performing auxiliary selection, the selection efficiency of high-yield breeding can be greatly improved.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and genetic breeding, and more specifically relates to a molecular marker CNU288 primer for the main effect gene locus of rapeseed grain weight, and also relates to the application of the molecular marker in high-yield breeding of rapeseed. Background technique [0002] Rapeseed is the largest oilseed crop in my country, accounting for about 20% of the world's rapeseed output. Rapeseed oil is the largest source of domestic edible vegetable oil, accounting for 57.2% of the total domestic edible vegetable oil, and occupies a very important position in the national edible oil supply security strategy. In addition, rapeseed oil and diesel oil have relatively similar fatty acid composition, which is a kind of green and renewable energy. As my country's urbanization scale continues to expand and the area of ​​arable land further shrinks, increasing the oil yield per unit area of ​​rapeseed (=...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 王汉中师家勤朱瑶瑶王新发刘贵华詹杰鹏
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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