Method for detecting pig viral diseases on basis of TEM-PCR and gene chip
A viral disease and gene chip technology, applied in the field of swine viral disease detection based on TEM-PCR and gene chip, to achieve the effects of good flexibility, saving experimental costs, and reducing background noise
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[0043] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0044] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.
[0045] Such as figure 1 Shown, the method for detecting porcine viral disease based on TEM-PCR and gene chip of the embodiment of the present invention may further comprise the steps:
[0046] S101: Preparation of DNA microarray: fix oligonucleotides on the substrate by in situ synthesis, use glass slides as the support of the gene chip, and before the polymerization reaction, the surface should be derivatized with hydroxyl or amino groups;
[0047] S102: Extract nucleic acid from ce...
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