A Molecular Marker Tightly Linked to the Qtl Locus of Kiwi Fruit Weight Traits and Its Application

A technology of kiwifruit and kiwifruit, which is applied in the fields of molecular biology and genetic breeding, can solve the problems that molecular marker research has not yet been carried out, and achieve the effects of convenient and fast detection methods, saving production costs, and improving selection efficiency

Active Publication Date: 2018-02-13
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the QTL mapping of important quality traits of kiwifruit fruit weight and the discovery of molecular markers related to fruit weight have not yet been carried out.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Acquisition of molecular markers linked to main effect QTL and development of kiwifruit fruit weight:

[0023] ① In this example, the kiwifruit 'MT570001' with a small fruit weight and the Chinese kiwifruit 'Guihai No. 4' with a large fruit weight were used to construct F 1 Separate groups. In the 6th year of fruiting of the population, select 150 F 1 The individual plants of the offspring of the population were the research objects.

[0024] ②A total of 150 individual plant leaves were collected from the population, and the total DNA was extracted by the CTAB method. According to the RADseq method, the libraries of Chinese kiwifruit 'Guihai 4', Shanli kiwifruit 'MT570001' and 150 offspring were constructed and sequenced.

[0025] ③ Filter the sequencing data to remove sequences with low-quality base content greater than 50%, and remove sequences with sequencing adapter contamination and a large number of repetitions. Use the software SOAP2 to compare the sequencing...

Embodiment 2

[0035] The application of a molecular marker closely linked to the QTL locus of kiwi fruit weight traits in kiwi fruit breeding, the steps are:

[0036] (1) F 1 Generation, select F 1 60 individual plants in the generation population were the research objects.

[0037] (2) At the ripening stage of the fruit, collect F 1 The fruit of 60 individual plants in the generation group, 40 fruits were collected from each plant, and the weight of the fruit was measured with a weighing balance to calculate the average fruit weight.

[0038] (3) Extract F 1 Generation of total DNA of 60 individual plants, according to Premix Ex Taq TM II (Tli RNaseHPlus), ROX plus kit instruction, design 20uL reaction system: 5ng·uL -1 Kiwifruit genomic DNA template 2ul, 2×SYBR Premix 10ul, 50×ROX Dye II 0.4ul, 10uM FW-F and FW-R each 0.8ul, double distilled water to make up the rest. The amplification program was: pre-denaturation at 95°C for 5 s, annealing at 60°C for 1 min; denaturation at 95°C...

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PUM

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Abstract

The invention discloses a molecular marker closely linked with a weight trait QTL site of a kiwifruit fruit and application of the molecular marker. Primers designed according to the molecular marker are FW-F:5'-CCTGAAGGGTTGACTGGAAA-3' and FW-R:5'-CTTGCAGGCATGAGGAAAGC-3'. The molecular marker firstly locates a main-effect QTL site for controlling fruit weight in a kiwifruit, can explain phenotypic variance of 16.24%; and the molecular marker associated with the fruit weight trait is detected, so that the weight of the kiwifruit fruit can be predicated, and therefore, an excellent individual plant with relatively great fruit weight can be accurately and quickly screened out.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and genetic breeding, and more specifically relates to a molecular marker closely linked with a QTL site for kiwi fruit weight traits and its application. Background technique [0002] Kiwi fruit is rich in vitamin C, dietary fiber and various mineral nutrients, and is a healthy fruit favored by consumers. China is the original origin center of the genus Actinidia. Actinidia is extremely rich in resources, with high morphological and genetic diversity. China has about 96% of the total resources of Actinidia species in the world. Relying on abundant natural resources, breeding new varieties with excellent traits plays a key role in the healthy and stable development of the kiwifruit industry. Fruit weight is one of the important factors affecting fruit quality, and it is also directly related to the unit yield of orchard and the economic income of fruit growers. Therefore, increasing fr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 黄宏文钟彩虹张琼陈美艳张琪
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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