Soybean lodging-resistant major gene locus and application

A technology for lodging and soybean resistance, applied in DNA/RNA fragmentation, microbial determination/inspection, recombinant DNA technology, etc., can solve the problems of poor repeatability, small QTL effect value, difficult application, etc., to improve selection efficiency, Accurate and rapid screening, the effect of speeding up the breeding process

Inactive Publication Date: 2013-05-08
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also some reports on the QTL mapping research on soybean lodging resistance, but the detected QTL effect value is small, and the repeatability is not good, so it is difficult to apply in soybean breeding

Method used

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  • Soybean lodging-resistant major gene locus and application
  • Soybean lodging-resistant major gene locus and application
  • Soybean lodging-resistant major gene locus and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Using the primer pair GmSSR13-27 to identify the F of Zhongdou 29×Zhongdou 32 11 lodging resistance traits

[0040] 1. PCR reagents for identification or auxiliary identification of soybean lodging resistance traits

[0041] The PCR reagent for identification or auxiliary identification of soybean lodging resistance traits in this embodiment consists of PCR primer pair GmSSR13-27, 10×Taq buffer, dNTP mixture, MgCl 2 solution, Taq DNA polymerase and ddH 2 O composition.

[0042] Among them, the PCR primer pair GmSSR13-27 consists of two single-stranded DNAs, a forward primer and a reverse primer, and its sequence is as follows:

[0043] Forward primer: 5'-TTTGTCGTATTTAGCTTCAGGC-3' (SEQ ID No.1),

[0044] Reverse primer: 5'-TCCACTTCTTATTTTCTTATTTGCG-3' (SEQ ID No.2).

[0045] 2. Soybeans to be identified

[0046] Middle Bean 29 × Middle Bean 32 F 11 The family was obtained as follows: Zhongdou 29 and Zhongdou 32 were crossed, and the offspring of the hyb...

Embodiment 2

[0114] Example 2, the acquisition of the lodging resistance main effect gene locus of soybean and the primer pair GmSSR13-27 of the present invention

[0115] The segregation population used was a cross between Zhongdou 29 (17.8%) and Zhongdou 32 (62.5%) with significant differences in lodging, and a soybean recombinant inbred line population containing 406 families was derived (Zhou Xinan et al., Soybean Recombination Three- and four-pod variation in inbred line populations and their relationship with yield, Chinese Journal of Oil Crops, 2005, 27: 22-25). The construction method of the isolated population is the same as the above-mentioned step 2.

[0116] 165 families were randomly selected from the recombinant inbred population for molecular marker analysis (Zhou Rong et al., QTL analysis of soybean seedling root traits, Acta Crops Sinica, 2011, 37:1151-1158) to obtain genotype data, using Joinmap3.0 Constructed a genetic map, combined with lodging rate data, used WinQTL c...

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Abstract

The invention discloses a soybean lodging-resistant major gene locus and an application, and provides the soybean lodging-resistant major gene locus and the application thereof in molecular marker-assisted selection breeding. The soybean lodging-resistant major gene locus is qLD.13-1 and the contribution rate is 37.6%. A molecular marker which is tightly interlocked with the soybean lodging-resistant major gene locus is GmSSR13-27. The tightly-interlocked molecular marker is used for detecting whether a breeding group family contains the major gene locus or not and the lodging resistance can be predicated; and the selection efficiency of soybean lodging-resistant breeding is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of soybean molecular biology and genetic breeding. More specifically, it relates to a soybean lodging resistance main effect gene locus and a molecular marker closely linked with the main effect gene locus, and also relates to the application of the molecular marker in soybean lodging resistance breeding. Background technique [0002] Soybean is an important crop, which occupies an important position in the development of my country's national economy, the composition of world food crops and the production of international oil crops. Plant lodging is a common and serious problem in soybean production, and it has become one of the main limiting factors to achieve high and stable yield and high quality of soybean. Especially in the late growth period, it is easy to cause lodging due to wind and rain. Lodging destroys the crop population structure, disturbs the normal distribution order of leaves in space, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 陈海峰沙爱华单志慧杨中路张晓娟张婵娟陈李淼陈水莲邱德珍周蓉吴学军周新安
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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