Molecular marker of pot shattering resistance trait major gene locus of rapes and application

A molecular marker and major gene technology, applied in the fields of molecular biology and genetics and breeding, can solve the problems that quantitative traits are easily affected by environmental conditions, long cycle, poor selection effect, etc., and achieve a convenient and fast detection method with high cost. , the effect of improving the selection efficiency
CN102747080AActive Publication Date: 2012-10-24INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
3 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Publication Date
2012-10-24

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a molecular marker of a pot shattering resistance trait major gene locus of rapes and an application. The steps are as follows: 1) hybridizing double 11 variety and 73290 variety (which have obvious differences in the pot shattering resistance traits) in a cabbage type rape varieties, and carrying out selfing on progenies to obtain F2 and F2:3 segregation populations with the segregated pod shattering resistance traits; 2) carrying out polymorphic screening on parent DNA (deoxyribonucleic acid) by utilizing an SSR (simple sequence repeat) primer, and establishing a genetic linkage map by carrying out SSR molecular marker genotyping on the F2 progeny segregation populations; 3) carrying out field experiments and plant inquisition on the F2 and F2:3 segregation populations so as to obtain phenotype data of the pod shattering resistance traits; and 4) carrying out QTL (quantitative trait locus) detection by combining with the developed high-density molecular marker genetic linkage map and genotype and the phenotype data of the segregation populations, utilizing QTL Cart2.5 software to obtain the major gene locus Psr.A9 for controlling the pod shattering resistance of the rapes on a cabbage type rape A9 linkage group and also obtain the molecular marker BrSF0007-39 closely linked with the major gene locus. The selection efficiency of breeding with the pod shattering resistance can be greatly improved by utilizing the marker for assistant selection.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of molecular biology and genetic breeding, and in particular relates to the main gene locus of the brassica napus anti-cracking angle trait and its closely linked molecular markers, and also relates to the application of the molecular markers in the breeding of rape with high cracking-horn resistance . Background technique

[0002] Rapeseed is the third largest oil crop in the world and the fifth largest crop in my country after rice, wheat, corn and soybean. About 13% of the world's vegetable oil comes from rapeseed. In addition to being the main raw material for edible oil, rapeseed is also an important industrial raw material and a major biological resource for renewable energy in the main member states of the European Union. The planting area and total output of my country's rapeseed account for about 30% of the world's total. It is not only related to the adjustment and optimization of my country's pla...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More