A Molecular Marker Primer and Application of the Main Effect Gene Locus of Rapeseed Seeds Per Corner Trait

A molecular marker and grain number technology, applied in the fields of molecular biology and genetic breeding, can solve the problems of high quality requirements, high cost, and difficult rapeseed breeding applications.
CN109680093BActive Publication Date: 2022-02-18OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
Publication Date
2022-02-18

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Abstract

The invention belongs to the technical field of molecular biology and genetic breeding, and specifically discloses a molecular marker primer and application of a main effect gene locus of the number of seeds per corner of rapeseed, through the F2 and F2:3 families of Zhongshuang 11 and 73290 The segregation population was subjected to field experiments and breeding tests to obtain the phenotypic data of the trait of kernel number per corner; combined with the genotype and genetic map of the F2 segregation population, QTL detection was carried out. Obtained the main effect gene loci controlling the number of seeds per corner of rapeseed on the A7 linkage group qSN.A7 and the molecular marker Ni201. Through the genotype analysis of the F3 generation derived from the two parents by this marker, the average number of grains per corner of the selected single plant carrying the favorable gene exceeds the average value of the single plant carrying the unfavorable gene, and the test results show that the single plant carrying the favorable marker has a The proportion of grain number higher than the average value of individual plants carrying unfavorable genes was as high as 88.0%, so the use of this marker for assisted selection can greatly improve the selection efficiency of high-yield breeding.
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Description

technical field

[0001] The invention belongs to the technical field of molecular biology and genetic breeding, and more specifically relates to a molecular marker primer for a main effect gene locus of the rape seed number per corner trait, and also relates to the application of the molecular marker in high-yield breeding of rapeseed. Background technique

[0002] Rapeseed is the largest oilseed crop in my country, accounting for about 20% of the world's rapeseed production (Hu et al., 2016). Rapeseed oil is the largest source of domestic edible vegetable oil, accounting for 57.2% of the total domestic edible vegetable oil, and occupies a very important position in the national edible oil supply security strategy (Fan Chengming et al., 2018). my country's foreign dependence on domestic vegetable oil has exceeded 60%, and there is a tendency to continue to increase (Wang Hanzhong et al., 2014). In addition, rapeseed oil and diesel oil have relatively similar fatty acid compo...

Claims

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