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Soybean anti-rust gene locus and application thereof

A soybean rust resistance and anti-rust technology, which is applied in the direction of DNA/RNA fragments, microbial measurement/inspection, recombinant DNA technology, etc., can solve the problems of no disease-resistant varieties and control methods, and achieve convenient and fast detection methods and save production cost, the effect of speeding up the breeding process

Active Publication Date: 2014-11-26
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2004, soybean rust officially landed in the continental United States and quickly spread to the north. Since there are no effective disease-resistant varieties and control methods, the annual loss is as high as 7 billion to 10 billion US dollars

Method used

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  • Soybean anti-rust gene locus and application thereof
  • Soybean anti-rust gene locus and application thereof
  • Soybean anti-rust gene locus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Using the primer pair GmSSR18-21 to identify the F of Zhongdou 40×SX6907 2 Individual plant resistance to rust

[0040] 1. PCR reagents for identification or auxiliary identification of soybean rust resistance

[0041] The PCR reagent for identification or auxiliary identification of soybean rust resistance in this embodiment consists of PCR primer pair GmSSR18-21, 10×Taq buffer, dNTP mixture, MgCl 2 solution, Taq DNA polymerase and ddH 2 O composition.

[0042] Among them, the PCR primer pair GmSSR18-21 consists of two single-stranded DNAs, a forward primer and a reverse primer, and its sequence is as follows:

[0043] Forward primer: 5'-ACCTCCTCCTCTCCCTGAAG-3' (SEQ ID NO: 1),

[0044] Reverse primer: 5'-CGGTTCAATTCTCAAAGGAGG-3' (SEQ ID NO: 2).

[0045] 2. Soybeans to be identified

[0046] Zhongdou 40×SX6907 F 2 The segregation population is obtained as follows: Zhongdou 40 and SX6907 are crossed, and the hybrid offspring are self-crossed, which is F ...

Embodiment 2

[0114] Embodiment 2, the acquisition of the soybean rust resistance gene locus of the present invention and the primer pair GmSSR18-21

[0115] The isolates used were the non-rust-resistant soybean variety Tianlong No. 1 (Chen Limiao et al., A study on the factors affecting the transformation of soybean cotyledon nodes using Agrobacterium-mediated transformation, Soybean Science, 2012, 1:17-23) and rust-resistant SX6907 (Shan Zhi Hui et al., Screening and identification of a new source of resistance to soybean rust, Chinese Journal of Oil Crops, 2012, 34: 188-192) crossed, and the hybrid offspring were self-crossed, that is, the F2 segregation population, with a total of 181 individual plants.

[0116] to F 2 The 181 individual plants were analyzed by SSR molecular markers to obtain genotype data, and the genetic map was constructed using Joinmap3.0, combined with the identification data of rust resistance inoculation, and the rust resistance gene was mapped using WinQTL carto...

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Abstract

The invention discloses a soybean anti-rust gene locus and application thereof, particularly a soybean anti-rust gene locus and application thereof in molecular marker assisted selective breeding. The anti-rust gene locus is rpp.18-1, and the molecular marker closely linked with the anti-rust gene locus is GmSSR18-21. The soybean rust resistance can be predicted by judging whether the closely linked molecular marker detection breeding material contains the major gene locus, thereby greatly enhancing the selection efficiency for soybean anti-rust breeding.

Description

technical field [0001] The invention relates to the field of biotechnology, and belongs to the technical field of soybean molecular biology and genetic breeding, in particular to a molecular marker tightly linked with soybean rust resistance gene loci, and also relates to the application of the molecular marker in soybean rust resistance breeding. Background technique [0002] Soybean is an important crop, rich in protein and fat and active ingredients beneficial to the human body such as soap II, isoflavones, phospholipids, etc. It is one of the main sources of vegetable protein and oil in the world. At present, my country's soybean planting area, total output and per unit yield rank fourth in the world, and its production level lags behind the world's soybean production. In 2012, China's soybean planting area was 7 million hectares, with a total output of 12.2 million tons, while imported soybeans reached a record 63.4 million tons, accounting for five-sixths of my country...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陈海峰单志慧杨中路沙爱华邱德珍张婵娟陈李淼袁松丽张晓娟赵胜陈水莲万乔周新安
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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