Wheat anti-gibberellic in-vitro directional selection breeding method
A technology of directional selection and wheat, which is applied in the field of crop resistance genetic breeding, can solve the problems that have not yet formed a simple, intuitive and reliable identification method for wheat head blight resistance, and achieves low selection probability, high selection efficiency, and selective population big effect
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Embodiment 1
[0037] 1. Isolation and cultivation of Fusarium graminearum strains
[0038] Directly isolate the Fusarium graminearum bacterial strain from the wheat disease ear infected with Fusarium graminearum, inoculate it in the grain culture medium, and cultivate it for 30 days at 25° C. under astigmatism conditions for toxin production. The production of the grain culture medium is as follows: 1. After soaking wheat grains without bacteria in warm water for 24 hours, heat them and boil them for 1 hour until the wheat grains are cooked but not rotten. 2 Autoclave for 90 minutes.
[0039] 2) Obtain crude toxin
[0040] The Fusarium toxin-producing wheat kernel culture cultured for 30 days was taken out from the culture bottle and then dried at a constant temperature of 60°C. 1000g of the dried product was extracted with ethyl acetate for 24hs, and the extracted product was subjected to G 5 Filter with funnel, evaporate to dryness at room temperature, dissolve the evaporated matter wit...
Embodiment 2
[0052] According to the operation steps in Example 1, the parent material Wangshuibai with the genotype resistant to gibberella was crossed with the excellent wheat variety Ningmai No. 6. At the same time, change the wheat anther callus induction medium, universal medium W 14 , anther callus differentiation medium, universal medium Ms, and formulas for inducing rooting and strong seedling medium.
[0053] Respectively, wheat anther callus induction medium, general medium W 14As a basis, add crude toxin: 0.8ml / L, 2,4-diaminophenoxyacetic acid: 1.8mg / L, 6-furylaminopurine: 0.4mg / L, thiamine hydrochloride: 7mg / L, tyrosine Protein: 490mg / L, biotin: 0.4mg / L, glutamine: 48mg / L, sucrose: 55000mg / L; maltose: 25000mg / L, agar powder: 4500mg / L; adjust the pH value of the induction medium to 5.5;
[0054] Universal Medium W 14 : Potassium nitrate: 1950mg / L, ammonium dihydrogen phosphate: 370mg / L, calcium chloride: 130mg / L, magnesium sulfate: 195mg / L, potassium sulfate: 690mg / L, ferrous...
Embodiment 3
[0058] According to the operation steps of Example 1, the genotype parental material Yining wheat and the excellent wheat variety Anhui No. 11 were crossed. At the same time, change the wheat anther callus induction medium, universal medium W 14 , anther callus differentiation medium, universal medium Ms, and formulas for inducing rooting and strong seedling medium.
[0059] Respectively, wheat anther callus induction medium, general medium W 14 As a basis, add the crude toxin obtained in step (2): 1.2ml / L, 2,4-diaminophenoxyacetic acid: 2.2mg / L, 6-furyl aminopurine: 0.6mg / L, thiamine hydrochloride : 9mg / L, casein: 510mg / L, biotin: 0.6mg / L, glutamine: 52mg / L, sucrose: 65000mg / L; maltose: 35000mg / L, agar powder: 5500mg / L; adjusted induction culture The base pH value is 5.7;
[0060] Universal Medium W 14 : Potassium nitrate: 2050mg / L, ammonium dihydrogen phosphate: 390mg / L, calcium chloride: 150mg / L, magnesium sulfate: 205mg / L, potassium sulfate: 710mg / L, ferrous sulfate: 2...
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