Wheat anti-gibberellic in-vitro directional selection breeding method

A technology of directional selection and wheat, which is applied in the field of crop resistance genetic breeding, can solve the problems that have not yet formed a simple, intuitive and reliable identification method for wheat head blight resistance, and achieves low selection probability, high selection efficiency, and selective population big effect

Inactive Publication Date: 2015-10-14
平顶山市平丰种业有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have been carried out on the changes in morphology or physiological characteristics caused by the crude toxin of Fusarium rubella, but most of them are only researched on a few or one of the indicators. Due to the different test materials and measurement periods, no simple, simple An Intuitive and Reliable Method Applied to Identification of Wheat Scab Resistance

Method used

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  • Wheat anti-gibberellic in-vitro directional selection breeding method
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  • Wheat anti-gibberellic in-vitro directional selection breeding method

Examples

Experimental program
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Effect test

Embodiment 1

[0037] 1. Isolation and cultivation of Fusarium graminearum strains

[0038] Directly isolate the Fusarium graminearum bacterial strain from the wheat disease ear infected with Fusarium graminearum, inoculate it in the grain culture medium, and cultivate it for 30 days at 25° C. under astigmatism conditions for toxin production. The production of the grain culture medium is as follows: 1. After soaking wheat grains without bacteria in warm water for 24 hours, heat them and boil them for 1 hour until the wheat grains are cooked but not rotten. 2 Autoclave for 90 minutes.

[0039] 2) Obtain crude toxin

[0040] The Fusarium toxin-producing wheat kernel culture cultured for 30 days was taken out from the culture bottle and then dried at a constant temperature of 60°C. 1000g of the dried product was extracted with ethyl acetate for 24hs, and the extracted product was subjected to G 5 Filter with funnel, evaporate to dryness at room temperature, dissolve the evaporated matter wit...

Embodiment 2

[0052] According to the operation steps in Example 1, the parent material Wangshuibai with the genotype resistant to gibberella was crossed with the excellent wheat variety Ningmai No. 6. At the same time, change the wheat anther callus induction medium, universal medium W 14 , anther callus differentiation medium, universal medium Ms, and formulas for inducing rooting and strong seedling medium.

[0053] Respectively, wheat anther callus induction medium, general medium W 14As a basis, add crude toxin: 0.8ml / L, 2,4-diaminophenoxyacetic acid: 1.8mg / L, 6-furylaminopurine: 0.4mg / L, thiamine hydrochloride: 7mg / L, tyrosine Protein: 490mg / L, biotin: 0.4mg / L, glutamine: 48mg / L, sucrose: 55000mg / L; maltose: 25000mg / L, agar powder: 4500mg / L; adjust the pH value of the induction medium to 5.5;

[0054] Universal Medium W 14 : Potassium nitrate: 1950mg / L, ammonium dihydrogen phosphate: 370mg / L, calcium chloride: 130mg / L, magnesium sulfate: 195mg / L, potassium sulfate: 690mg / L, ferrous...

Embodiment 3

[0058] According to the operation steps of Example 1, the genotype parental material Yining wheat and the excellent wheat variety Anhui No. 11 were crossed. At the same time, change the wheat anther callus induction medium, universal medium W 14 , anther callus differentiation medium, universal medium Ms, and formulas for inducing rooting and strong seedling medium.

[0059] Respectively, wheat anther callus induction medium, general medium W 14 As a basis, add the crude toxin obtained in step (2): 1.2ml / L, 2,4-diaminophenoxyacetic acid: 2.2mg / L, 6-furyl aminopurine: 0.6mg / L, thiamine hydrochloride : 9mg / L, casein: 510mg / L, biotin: 0.6mg / L, glutamine: 52mg / L, sucrose: 65000mg / L; maltose: 35000mg / L, agar powder: 5500mg / L; adjusted induction culture The base pH value is 5.7;

[0060] Universal Medium W 14 : Potassium nitrate: 2050mg / L, ammonium dihydrogen phosphate: 390mg / L, calcium chloride: 150mg / L, magnesium sulfate: 205mg / L, potassium sulfate: 710mg / L, ferrous sulfate: 2...

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Abstract

The invention belongs to the technical field of crop disease-resistance genetic breeding, and provides a wheat anti-gibberellic in-vitro directional selection breeding method, which comprises: (1) Fusarium graminearum strain separation and culture; (2) crude toxin obtaining; (3) hybridization F1 generation seed obtaining; (4) resistant microspore arrhenokaryon development; (5) resistant callus differentiation; (6) haploid seedling obtaining; (7) anti-gibberellic strain obtaining; and (8) anti-gibberellic characteristic identification on the doubled haploid individual so as to screen the stable anti-gibberellic strain line. According to the present invention, the toxin is used to stress the wide microspore genotype variation and massive microspore cell populations due to gene recombination in the F1 generation anther tissue, the sensitivity difference of the resistant genotype microspore and the non-resistant genotype microspore on toxin is used to screen the resistant genotype, the selection characteristic is directional, the selected groups are large, and the selection efficiency is high.

Description

technical field [0001] The invention belongs to the field of genetic breeding for disease resistance of crops, and relates to a method for in vitro directional selective breeding of wheat resistant to gibberella. Background technique [0002] Scab is one of the three major diseases of wheat. It mainly occurs in the low-humidity areas of the Yangtze River Basin and Heilongjiang Province in my country. With the increase of irrigation area and improvement of cultivation conditions in the Huanghuai wheat region, it has spread to Huanghuai wheat since the 1970s. area and is rapidly becoming popular. In 1985, the disease was prevalent in the Huanghuai wheat region, affecting 80 million mu and losing 1.35 billion jin of wheat. Therefore, wheat scab has become a major obstacle to the sustainable and high-quality development of wheat production in my country. [0003] At present, no type of immunity to Gibberella has been found, but there are significant differences in resistance amo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04
Inventor 乔保建陈耀峰陶颖张鸿雁曹秀敏
Owner 平顶山市平丰种业有限责任公司
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