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Plant induced mutation breeding method for enhancing mutation frequency and mutation spectrum

A technology of mutation breeding and mutation frequency, which is applied in the fields of botany equipment and methods, plant products, mutant preparation, etc., can solve the problems that have not been reported, and achieve the goal of accelerating the breeding program, increasing the mutation frequency, and shortening the breeding cycle Effect

Active Publication Date: 2013-07-03
石春鸿 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

How to increase the frequency of sweet potato mutations and obtain more mutant individuals through mutation breeding methods, from which new varieties with target resistance qualities have not been reported.

Method used

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  • Plant induced mutation breeding method for enhancing mutation frequency and mutation spectrum
  • Plant induced mutation breeding method for enhancing mutation frequency and mutation spectrum
  • Plant induced mutation breeding method for enhancing mutation frequency and mutation spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: A large number of sweet potato embryogenic cell clusters occur

[0041] 1.1. Materials and methods

[0042] 1.1.1. Induction materials for sweet potato sterile seedlings:

[0043] MS medium, Murashige T and Skoog F A revised medium for rapid growth and bioassays with tobacco tissue cultures. (1962) Physiol Plant 15(3): 473-497.

[0044] Reagent:

[0045] 2,4-D: (2,4-Dichlorophenoxyacetic acid) Beijing Puboxin Biotechnology Co., Ltd.

[0046] BA: (6-benzyl adenine) Beijing Jyuzhou Tongye Biotechnology Co., Ltd.

[0047] 1AA: (Indoleacetic acid) Beijing Jiuzhou Tongye Biotechnology Co., Ltd.

[0048] NOA: (Naphthyloxyacetic acid) Beijing Jiuzhou Tongye Biotechnology Co., Ltd.

[0049] NAA (α-naphthaleneacetic acid) Beijing Jiuzhou Tongye Biotechnology Co., Ltd.

[0050] KT, (Kinetin) Beijing Jiuzhou Tongye Biotechnology Co., Ltd.

[0051] Paclobutrazol: Beijing Jiuzhou Tongye Biotechnology Co., Ltd.

[0052] GA3 (Gibberellin) Beijing Jiuzhou Tongye Biot...

Embodiment 2

[0070] Example 2: 60 Co-γ-ray Irradiation Treatment of Sweet Potato Stem and Embryogenic Cells

[0071] 2.1. Materials and methods

[0072] 2.1.1. Preparation of irradiation treatment material: use the sweet potato embryogenic cell line established by the present invention as material, select well-developed, and be in vigorous division state (callus granular, dense, bright yellow in color and luster, young and tender in texture) The callus was transferred to fresh subculture medium (MS+2, 4-D 0.05-0.2mg / L+6-BA 0.5-1mg / L+KT0.5-1mg / L+NOA0. 5-1mg / L+IAA0.5-1mg / L+TDZ0.5mg / L). Irradiation was carried out 10-15 days after transfer to the subculture medium.

[0073] 2.1.2. Irradiation treatment method: Send the embryogenic callus cultured in vitro to the Institute of Atomic Energy, Chinese Academy of Agricultural Sciences 60 Co-γ-ray irradiation treatment, the radiation dose is 10, 20, 30, 40, 60Gy, and 4 bottles of callus are used for each treatment. After irradiation, the embry...

Embodiment 3

[0079] Example 3: Induction of meiosis in sweet potato embryogenic cells

[0080] 3.1 Materials and methods:

[0081] 3.1.1. Materials:

[0082] One of the test materials used in the present invention is golden leaf potato. Golden leaf potato is an annual ground cover newly developed in recent years. Because of its golden and translucent color, it is very eye-catching, especially in summer, and it can play a very important role in flowers. Good foil.

[0083] After the dedifferentiation treatment described in Example 1 and the irradiation mutagenesis treatment described in Example 2, the embryogenic cells of Auranthus chinensis were obtained for the meiosis-like induction experiment in this implementation.

[0084] 3.1.2. Method

[0085] Optimal conditions for mitotic index under treatment with purine nucleosides

[0086] Taking sweet potato embryogenic cells without drug treatment as a control, observe the mitotic index (the percentage of dividing cells in 100 cells) and ...

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Abstract

The invention relates to a plant induced mutation breeding method for enhancing mutation frequency and mutation spectrum, belonging to the field of induced mutation breeding. The method comprises the following steps: (1) induced mutation: carrying out induced mutation on embryonic cells, and carrying out secondary amplification culture; (2) meiosis-like induction: carrying out meiosis-like induction on the cells subjected to induced mutation and secondary amplification culture; and (3) naturally doubling to obtain the mutant homozygote cells, and screening the mutant homozygote by combining the breeding objective and mutant characters. The invention can be used for screening a great deal of materials in vitro within a short time to obtain a unicell-derived homozygote stress-tolerant mutant, thereby obviously shortening the breeding period and greatly enhancing the selection efficiency.

Description

technical field [0001] The invention relates to a mutation breeding technique, in particular to a plant mutation breeding method for increasing mutation frequency and mutation spectrum. Background technique [0002] Carry out new variety breeding with radiation mutagenesis technology, existing many reports, though this method breeding has many successful precedents, the method is labor-intensive and time-consuming, and the breeding efficiency is low, especially for the mutation breeding of asexually propagated plants, Since recessive mutations are difficult to reveal, the probability of obtaining target mutant individuals is very low. [0003] Characteristics of mutation breeding of asexually propagated plants: Generally speaking, when a dominant mutation occurs in a gene, it will appear in the present generation and form a chimera. However, the gene mutations induced by γ-rays are mostly recessive mutations, and only a small amount of dominant mutations. When using γ-ray r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/01A01H5/00
Inventor 石春鸿
Owner 石春鸿
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