Method for cultivating giant embryo japonica rice variety by editing rice giant embryo gene GE

Abstract

The invention discloses a method for cultivating a giant embryo japonica rice variety by editing a rice giant embryo gene GE. The giant embryo gene GE for controlling the size of a rice embryo is subjected to fixed-point editing through a CRISPR-Cas9 multi-target system, japonica rice genetic transformation is carried out to obtain a giant embryo transgenic plant, and transgenic insertion fragment detection is carried out on offspring, so that the breeding period can be shortened, and various large and small embryo japonica rice varieties without transgenic fragment insertion can be rapidly obtained; and the GABA content of brown rice of gene ge type mutant strains with different embryo sizes is also different, the GABA content is also increased along with the increase of embryos, and the rice varieties with different embryo weights can meet the requirement for extracting GABA with different content so as to meet the requirements of different consumer groups. Therefore, the invention can provide an efficient gene knockout method and a method for cultivating a plurality of giant embryo japonica rice varieties for production and application with different GABA content requirements.

Description

technical field [0001] The invention relates to the field of plant transgenic technology and crop genetic breeding, in particular to a method for editing rice giant embryo genes GE A method for cultivating giant-embryo japonica rice varieties; in particular, a method for editing rice using CRISPR-Cas9 multi-target technology GE genetically acquired variety GE The allele type mutant produces a variety of giant embryo materials whose seed embryos are larger than the wild type, and a breeding method for cultivating multiple giant embryo japonica rice varieties without insertion of transgene segments. Background technique [0002] Rice is an important food crop in my country and the staple food of more than 50% of the population in China. In recent years, with the improvement of people's living standards, people have higher pursuit of rice nutritional quality. Functional rice refers to rice that is rich in some physiologically active substances in addition to the nutritional c...

AI Technical Summary

Problems solved by technology

At present, giant embryo rice is mainly obtained by mutagenesis of the gene and hybrid breeding technology. However, these breeding methods require high cost, high labor intensity and long breeding cycle, and the size distribution of the obtained giant embryo rice seed embryos is limited.

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