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Simple screening method for wheat gene producing unreduced gamete

A screening method and unreduced technology, applied in genetic breeding and biological fields, can solve problems such as difficulty in screening wheat genotypes, difficulty in distant hybridization, and cumbersome screening process, so as to promote practical application efficiency, improve breeding efficiency, and avoid separation Effect

Active Publication Date: 2007-07-18
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the distant relationship between the parents, such distant hybridization is difficult, and it is possible to obtain hybrid seeds only through techniques such as immature embryo rescue.
Therefore, such a screening process is cumbersome and has a large workload, and it is very difficult to screen out the desired wheat genotype from a huge wheat population.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Transform the meiotic recombinant gene of tetraploid wheat landrace AS2255 into hexaploid wheat SHW-L1:

[0026] We crossed the Chinese tetraploid wheat landrace AS2255 (Triticum turgidum ssp.turgidum) with Jiejiemai AS60 to obtain hybrid seed F 1 . These F 1 F obtained after seed germination 1 The hybrid plant has 21 chromosomes and chromosome ABD (14 AB chromosomes from tetraploid wheat AS2255, and 7 D chromosomes from Jiejie wheat AS60). In theory, such F 1 The chromosomes of hybrid plants are randomly separated during meiosis, and the possibility of obtaining male and female gametes with 21 chromosomes is extremely small. Therefore, the frequency of normal viable gametes is very low, so such F 1 Selfing of plants is usually not fruitful. However, we found in the first year that the F from AS2255 and AS60 1 48 seeds were obtained from the 238 florets selfed by the plant, and the seed setting rate was as high as 20.17%. In the second year, we repeated this work...

Embodiment 2

[0032] Example 2: Distant hybridization of wheat SHW-L1 with meiotic recombination gene and Aegilops variabilis:

[0033] According to the normal method of crossing two common wheat varieties, that is, without special methods such as tissue culture, we used SHW-L1 as the female parent and Aegilops variabilis AS24 as the male parent in 2003 and 2005, respectively, and obtained 30.68% and 48.37 respectively. % Hybrid seed setting rate (Table 2), indicating that the hybrid combination is easy to obtain hybrids. At the same time, we used Aegilops variabilis AS24 as the female parent and SHW-L1 as the male parent in 2003 and 2005, and obtained 9.09% and 9.62% hybrid seed setting rates respectively. See Table 2 for details, indicating that reverse crosses can also be successful. Germination experiments on these hybrids showed that the hybrid seeds obtained by the combination of SHW-L1 and Aegilops variabilisAS24 were easy to germinate.

[0034] Table 2. Hybridization of SHW-L1 and Aegil...

Embodiment 3

[0036] Example 3: Selfing of a distant hybrid of wheat without meiotic recombination gene and Aegilops variabilis hardly bears fruit:

[0037] We used the popular wheat varieties Chuanyu 16, Chuanmai 28 without meiotic recombination genes, the new wheat line TKL1, the wheat landrace Xinzhongchang, Kaixian Luohanmai, China Spring and ph1b mutants, etc., and distant crosses with Aegilops variabilis , All showed ideal hybridability, and hybrids were easily obtained (Table 3), indicating that there are no hybridization obstacles in the distant hybridization between wheat and Aegilops variabilis.

[0038] Table 3. The hybridization of wheat and Aegilops variabilis

[0039] Parent material

[0040] The genome of wheat is AABBDD (2n=6x=42), and the genome of Aegilops variabilis is UUS 1 S 1 (2n=4x=28), their hybrid F 1 Plants usually have 35 chromosomes, and its genome is ABDUS 1 , Which is composed of A, B, D, U, S 1 A haploid composed of five chromosomes. These chromosomes al...

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PUM

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Abstract

A simple method for screening the wheat genotype able to generate unreduced gamete for breaking through the 'reduplicative bottle neck' of reduplicating monoploid includes such steps as distant hybridizing between wheat and Aegilops variabilis to obtain plant F1, selfing, and screening.

Description

Technical field [0001] The invention relates to the fields of genetic breeding and biotechnology, in particular to a simple screening method for wheat genotypes that produce unreduced gametes in a haploid state. Background technique [0002] Normally, plant somatic cells contain two sets of chromosomes, respectively derived from male and female gametes, so the number of chromosomes is double (2n). Haploid is a plant with gamete chromosome composition and only contains half (n) of the number of chromosomes of normal somatic cells. Common wheat (chromosome group is AABBDD, 2n=6x=42) is a heterohexaploid (2n=6x=42) composed of three chromosomes A, B, and D. Each chromosome group has 7 pairs of chromosomes. The A and B chromosomes of wheat are provided by tetraploid wheat (Triticum turgidum L., the chromosome is AABB, 2n=28), while the D chromosome is provided by diploid nodules (some called Aegilops tauschii Cosson). Or Triticum tauschii (Cosson) Schmalh. or Aegilops squarrosa L, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01G7/00
Inventor 刘登才张连全颜泽洪郑有良
Owner SICHUAN AGRI UNIV
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