10 results about "Aegilops tauschii" patented technology

The application belongs to the technical field of genetic engineering, and particularly relates to a ten-ploid long-spike Aegilops tauschii introgression gene ThpFBX1 and application thereof in regulating the heading stage of wheat. The amino acid sequence of the ten-ploid long-spike Aegilops tauschii introgression gene ThpFBX1 is shown as SEQ ID No. 1, and the nucleotide sequence is shown as SEQ ID No. 2. The ten-ploid long-spike Aegilops tauschii introgression gene ThpFBX1 is introduced into a recipient plant to obtain a transgenic plant overexpressing the ThpFBX1 gene, and experiments show that the overexpressing wheat exhibits early heading, and the ThpFBX1 gene can significantly advance the heading stage of the wheat.

This invention relates to a powdery mildew resistance gene PmAeu1 derived from *Aegilops spp.*, its molecular markers, and applications, belonging to the field of genetic engineering. This invention utilizes the powdery mildew-resistant *Aegilops spp.* strain AE 1617 and the powdery mildew-susceptible strain AE 538 to construct mapping populations. Through BSR-Seq, fine genetic mapping, third-generation genome sequencing, and transgenic verification, a new powdery mildew resistance gene, PmAeu1, was cloned. This invention has demonstrated through transgenic wheat research that the PmAeu1 gene can significantly improve wheat resistance to powdery mildew. The *Aegilops spp.* powdery mildew resistance gene PmAeu1 can be applied to genetically improve wheat varieties to enhance wheat's powdery mildew resistance. This invention also develops a molecular marker, XAeuNLR1-2, for the PmAeu1 gene, which can be used to specifically detect the gene PmAeu1 as described in the claims and can be applied to marker-assisted breeding of the PmAeu1 gene.

The invention discloses a candidate gene AetNLR2CIae8 of a powdery mildew resistant gene pmAeCIae82DS derived from aegilops tauschii as well as a protein coded by the candidate gene AetNLR2CIae8 and application of the candidate gene AetNLR2CIae8, and the nucleotide sequence of the candidate gene AetNLR2CIae8 of the powdery mildew resistant gene derived from the aegilops tauschii is as shown in SEQ ID NO. 1. 1, and amino acid sequences coded by the gene are respectively as shown in SEQ ID NO. 2. The pmAeCIae82DS gene sequence belongs to the protection range of the invention. The invention also protects the application of the candidate gene AetNLR2CIae8 of the pmAeCIae82DS in the improvement of the disease resistance of the plants. The AetNLR2CIae8 is transformed into the susceptible wheat variety Yangmai 158 by utilizing a transient overexpression system, so that the aspirator index of powdery mildew can be obviously reduced; when the VIGS system is used for respectively silencing AetNLR2CIae8 in the aegilops sphaeroides powdery mildew resistant material TX11-1 containing the pmAeCIae82DS, the susceptibility of plants can be improved. Therefore, the AetNLR2CIae8 can positively regulate and control the wheat powdery mildew resistance and is an important gene resource for wheat disease-resistant breeding.

The present invention discloses a molecular marker related to the early heading trait of wheat and its application, belonging to the field of molecular genetic breeding. The nucleotide sequence of the molecular marker is shown in SEQ ID NO.1, and there is a G / A mutation at position 25. The genotype of this site includes GG, AA and GA genotypes. The molecular marker provided by the present invention is used in conjunction with the early heading mutant AS2388 of Aegilops tauschii. eh The molecular markers provided by the present invention are highly significantly correlated with the early heading gene ehd1 in wheat, exhibiting tightly linked marker characteristics. They are highly accurate for molecular marker-assisted selection and can significantly improve the efficiency of selecting and identifying materials containing the ehd1 early heading gene in different genetic backgrounds, with a high success rate. The molecular markers provided by the present invention can be used to locate the early heading trait of wheat, and can also be used to quickly screen plants containing the ehd1 gene during breeding, improving breeding efficiency and providing a foundation for research on early heading genes in wheat.

The invention discloses a tissue culture method of aegilops tauschii. Comprising the following steps: (1) disinfecting an explant, namely disinfecting a mature embryo of aegilops tauschii as the explant; (2) induction and subculture of calluses: inoculating the explants on an induction culture medium, and carrying out dark culture to induce embryogenic calluses; (3) differentiation of the calluses: transferring the calluses into a differential culture medium, and differentiating to obtain bud seedlings; (4) rooting: transferring the bud seedlings to a rooting culture medium to differentiate and root; and (5) hardening seedlings and transplanting after complete plants are formed. The efficient tissue culture regeneration system of aegilops tauschii is constructed for the first time, mature embryos of aegilops tauschii are selected as explants, materials are easy to obtain, the cost is low, and a large number of calluses can be produced by single seed; the method has the advantages of simple flow, strong repeatability and high differentiation rate, and lays a stable platform for genetic transformation and gene editing.

The application discloses a tetraploid long-spike Aegilops tauschii salt-tolerant 3EL chromosome molecular marker and application thereof, and belongs to the technical field of crop genetic breeding. LTTE3EM-2 and LTTE3EZ-1 ) and a specific molecular marker of a long arm of a 6A chromosome of common wheat ( LTTAE6A-2 ) are combined into two groups of molecular markers ( LTTE3EM-2 and LTTAE6A-2, LTTE3EZ-1 and LTTAE6A-2 ), and a primer pair is designed based on the two groups of molecular markers, and it is found through verification that the designed primer pair can be used for identifying the genotype of a salt-tolerant wheat-tetraploid long-spike Aegilops tauschii 3EL translocation line, that is, whether the tetraploid long-spike Aegilops tauschii salt-tolerant 3EL chromosome is homozygous or not can be identified. The application provides an important way for wheat tribe system evolution, germplasm resource identification and molecular marker assisted selection breeding.

The application discloses KASP primers of the short arm of 2D chromosome of Aegilops tauschii and application thereof, and belongs to the technical field of wheat molecular breeding. The KASP primers of the short arm of 2D chromosome of Aegilops tauschii comprise five groups of primers and can be used for identifying fragments derived from the short arm of 2D chromosome of Aegilops tauschii. The identification method comprises the following steps: (1) extracting wheat genomic DNA; (2) mixing the five groups of primers with the wheat genomic DNA and KASP reaction liquid respectively, and performing KASP amplification and reading fluorescence signals; and (3) judging whether the detected wheat genomic DNA contains fragments derived from the short arm of 2D chromosome of Aegilops tauschii according to the fluorescence signals. The KASP primers can be used for rapidly screening breeding materials with high photosynthetic efficiency and high yield potential in weak light areas.

The invention discloses a primer group for detecting a KASP molecular marker related to the grain protein content and application of the primer group, and belongs to the technical field of biology. The wheat-aegilops tauschii introgression line and the secondary segregation population are utilized to finely locate a new major QTL existing in the aegilops tauschii parent and the aegilops tauschii introgression line, and the synergistic gene comes from aegilops tauschii, is not widely utilized by breeders, and has great potential application value. The invention also further develops a KASP molecular marker CS4D-19405835 which is closely linked with the content of the grain protein. High-quality germplasm resources, functional genes and molecular markers are provided for excavation and application of excellent-quality genes of aegilops tauschii, and wheat quality improvement is assisted.

The invention discloses a KASP primer of a 2D chromosome short arm of aegilops tauschii and application of the KASP primer, and belongs to the technical field of wheat molecular breeding. The KASP primer for the short arm of the 2D chromosome of the aegilops tauschii comprises five groups of primers and can be used for identifying fragments derived from the short arm of the 2D chromosome of the aegilops tauschii. The identification method comprises the following steps: (1) extracting wheat genome DNA; (2) respectively mixing the five groups of primers with wheat genome DNA and a KASP reaction solution, carrying out KASP amplification, and reading a fluorescence signal; and (3) judging whether the detected wheat genome contains a fragment derived from the short arm of the 2D chromosome of the aegilops tauschii or not according to the fluorescence signal. The breeding material with high photosynthetic efficiency and high yield potential in a weak illumination region can be rapidly screened through the KASP primer.

The application belongs to the field of plant biotechnology and wheat molecular breeding, and discloses a functional molecular marker for detecting a wheat powdery mildew resistance gene WTK4 and application thereof. The wheat powdery mildew resistance gene WTK4 is located on the short arm of the 7D chromosome of Aegilops tauschii, and the WTK4 homologous gene is located on the long arm of the 4A chromosome in the Chinese spring reference genome RefSeq v2.0 version. The functional molecular marker difference site is located inside the WTK4 gene, and the polymorphism is an insertion / deletion (Indel) of 105 bp. The molecular marker provided in the application can accurately detect whether the powdery mildew resistance gene WTK4 exists in the material, and the material containing the WTK4 gene can be distinguished into the Aegilops tauschii disease-resistant genotype and the Chinese spring disease-sensitive genotype according to the size of the amplification band. The application promotes the utilization of the wheat powdery mildew resistance gene WTK4, and can be applied to molecular marker-assisted selection breeding, and accelerates the breeding process of the wheat disease-resistant variety.