Puccinia malvacearum-resistant gene pmaeu1 in aegilops tauschii and molecular markers and application thereof

By constructing a map population of Aegilops spp. and using high-throughput sequencing technology, a new wheat powdery mildew resistance gene, PmAeu1, and its molecular marker were cloned. This solved the problem of scarce wheat resistance gene resources, significantly improved wheat resistance to powdery mildew, and provided an important breeding foundation.

CN118834884BActive Publication Date: 2026-03-03JIANGSU UNIV
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Patent Information

Application Number
CN202411075569.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-08-07
Publication Date
2026-03-03
Estimated Expiration
2044-08-07

AI Technical Summary

Technical Problem

The scarcity of existing resistance gene resources has led to the loss of wheat's resistance to powdery mildew, making it difficult to continuously improve wheat's disease resistance.

Method used

By constructing mapping populations of the powdery mildew-resistant Aegilops spp. AE 1617 and the powdery mildew-susceptible Aegilops spp. AE 538, the powdery mildew resistance gene PmAeu1 was cloned using BSR-Seq, fine genetic mapping, and third-generation genome sequencing technologies. The corresponding molecular marker XAeuNLR1-2 was also developed for specific detection and assisted breeding.

Benefits of technology

A new powdery mildew resistance gene, PmAeu1, was successfully cloned, encoding a CC-NB-LRR class of resistance protein. This significantly improves wheat resistance to powdery mildew, providing important genetic resources and a breeding foundation, and enabling efficient disease resistance gene-assisted breeding.

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Abstract

This invention relates to a powdery mildew resistance gene PmAeu1 derived from *Aegilops spp.*, its molecular markers, and applications, belonging to the field of genetic engineering. This invention utilizes the powdery mildew-resistant *Aegilops spp.* strain AE 1617 and the powdery mildew-susceptible strain AE 538 to construct mapping populations. Through BSR-Seq, fine genetic mapping, third-generation genome sequencing, and transgenic verification, a new powdery mildew resistance gene, PmAeu1, was cloned. This invention has demonstrated through transgenic wheat research that the PmAeu1 gene can significantly improve wheat resistance to powdery mildew. The *Aegilops spp.* powdery mildew resistance gene PmAeu1 can be applied to genetically improve wheat varieties to enhance wheat's powdery mildew resistance. This invention also develops a molecular marker, XAeuNLR1-2, for the PmAeu1 gene, which can be used to specifically detect the gene PmAeu1 as described in the claims and can be applied to marker-assisted breeding of the PmAeu1 gene.
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