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Gossypium-barbadense-Hai-1-derived molecular markers related to fiber Micronaire value and application thereof

A fiber micronaire and molecular marker technology, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of long cycle, slow progress in fiber quality breeding, and poor accuracy.

Active Publication Date: 2015-12-23
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to overcome many shortcomings such as poor accuracy of phenotypic selection, long cycle and low efficiency in traditional breeding, and solve the problem of slow progress in fiber quality breeding

Method used

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  • Gossypium-barbadense-Hai-1-derived molecular markers related to fiber Micronaire value and application thereof
  • Gossypium-barbadense-Hai-1-derived molecular markers related to fiber Micronaire value and application thereof
  • Gossypium-barbadense-Hai-1-derived molecular markers related to fiber Micronaire value and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Screening Molecular Markers

[0044] (1) Construction of introgression lines and acquisition of phenotypic data

[0045] Using Haidao Mianhai 1 as the donor parent and Zhongmian 36 as the recurrent parent, a high-generation backcross introgression line population of land-sea hybrids was constructed. The recurrent parent Zhongmian Research Institute 36 (CCRI36) is an early-maturing and excellent upland cotton variety (National Approved Cotton 990007) bred by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences, and the donor parent Hai1 (Hai1) is a dominant aglandular gene Sea-island cotton cultivars with high Verticillium wilt resistance and excellent fiber quality characteristics.

[0046] In the summer of 2003, F 1 , in the winter of the same year in Sanya, Hainan, backcrossed BC with Zhongmian Institute 36 as the female parent 1 f 1 ; From 2004 to 2008, through continuous backcrossing and self-crossing, 133 BCs were obtained 5 ...

Embodiment 2

[0068] Example 2 Molecular marker selection method for the reduction of upland cotton fiber micronaire value traits

[0069] Using the molecular marker BNL3145 obtained in Example 1 330 and HAU0764 220 Molecular marker selection is carried out in the breeding population related to Sea Island Cotton Sea 1, etc., including the following steps:

[0070] (1) DNA extraction: take Sea Island Cotton Sea 1 as the donor parent, and upland cotton varieties or strains (such as Lumianyan 28, Zhongmian Institute 60) as the recipient parent, carry out hybridization and backcrossing to obtain segregated populations, or use Haidao Mianhai 1 is the donor parent, and the upland cotton varieties (such as Lumianyan 28 and Zhongmiansuo 60) are the introgression lines and their derivatives obtained by the high-generation backcrossing of the recipient parents, or the introgression lines and the land cotton varieties. For the offspring populations of hybridization and backcrossing of cotton varieti...

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Abstract

The invention relates to the technical field of molecular breeding, particularly Gossypium-barbadense-Hai-1-derived molecular markers related to fiber Micronaire value and application thereof. The molecular markers are BNL3145330 and HAU0764220. The molecular markers are beneficial to overcoming the defects in the fiber quality identification in the existing breeding technique, and can enhance the fiber Micronaire value selection efficiency and accelerate the culturing progress of high-quality new species.

Description

technical field [0001] The invention relates to the technical field of cotton molecular breeding, in particular to a molecular marker related to fiber micronaire value from Sea Island Cotton Sea 1 and its application. Background technique [0002] With the development of the textile industry and the improvement of people's living standards, higher requirements are put forward for the quality of cotton fibers, especially for fiber strength and fineness, and it is required to breed stronger, thinner and more neat new cotton varieties ( Xiang Shikang, et al., 1999). At present, Micronaire value is generally used as the evaluation index of fiber fineness, which is a comprehensive reflection of fiber fineness and maturity (Joseph, et al., 2005). The micronaire value is divided into three grades: A, B, and C, and grade B is the standard grade. The value range of A grade is 3.7-4.2, the quality is the best; the value range of B grade is 3.5-3.6 and 4.3-4.9; the value range of C g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 石玉真袁友禄宋威武檀运娜何蕊张金凤刘爱英李俊文巩万奎王涛龚举武商海红陈婷婷葛群
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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