7 results about "Gossypium barbadense" patented technology

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

The application discloses a sea island cotton chromosome fragment capable of improving fiber strength of upland cotton and application of the sea island cotton chromosome fragment. The application provides a chromosome fragment from sea island cotton, which is a fragment located from InDel molecular marker C6STR-3 to InDel molecular marker C6STR-10 on D06 chromosome of sea island cotton. The nucleotide sequence of the InDel molecular marker C6STR-3 is shown as SEQ ID NO. 3. The nucleotide sequence of the InDel molecular marker C6STR-10 is shown as SEQ ID NO. 6. The sea island cotton chromosome fragment provided by the application can significantly increase fiber strength of upland cotton when applied to cotton molecular breeding. Meanwhile, the InDel molecular marker developed by the application provides a molecular basis for breeding of high-fiber-strength cotton varieties.

This invention provides the application of the GbWAK5 gene in regulating the salt tolerance of sea island cotton, belonging to the field of plant genetic engineering technology. The nucleotide sequence of the GbWAK5 gene described in this invention is shown in SEQ ID NO.1, and the amino acid sequence of the protein encoded by the GbWAK5 gene is shown in SEQ ID NO.2. VIGS experiments have demonstrated that the GbWAK5 gene participates in the salt tolerance of sea island cotton by regulating ion homeostasis, and sea island cotton containing or highly expressing the GbWAK5 gene exhibits better salt tolerance. These results provide important information for understanding the members of the sea island cotton WAK gene family and provide potential target genes for further breeding of salt-tolerant varieties.

The invention relates to application of a GbPIF3 gene functional structure domain in plant type regulation, which is characterized in that a plant type related gene Gb-PIF3 is obtained by cloning from an apical meristem in a hybrid F1 of sea island cotton-upland cotton, and the functional structure domain GbPIL of the gene is subjected to heterologous expression in arabidopsis thaliana through a transgenic technology. The photoresponse test and seedling property observation on the obtained transgenic material prove that the gene functional structure domain has the function in plant type regulation and control, can be used for transforming the plant type structure of upland cotton and regulating and controlling the flowering time, and has important significance on the improvement of the yield and quality of cotton, especially the genetic modification of close-planting cotton.

The application relates to a cotton fiber strength major gene NTL9 BS41 and application thereof. Through cotton transgenic technology, NTL9 BS41 is successfully introduced into Gossypium hirsutum and high expression of the gene in cotton fibers is realized, the fiber strength of cotton is significantly enhanced without negative influence on other fiber qualities, overexpression of NTL9 BS41 can effectively enhance the density and uniformity of cellulose microfibril accumulation, and the structure and performance of cotton fibers are significantly optimized. In addition, the gene is derived from Gossypium barbadense, compared with Gossypium hirsutum, there is an insertion type variation of 24 bp nucleotides in the CDS of the gene, and a molecular marker capable of effectively distinguishing Gossypium hirsutum and Gossypium barbadense haplotypes is successfully developed. The application provides new gene resources, technical means and germplasm resources for cotton fiber quality improvement, and has far-reaching application value and potential for promoting the progress of cotton breeding work.

This invention discloses a sea island cotton GbUNE12 The application of genes in regulating cotton resistance to Verticillium wilt, the aforementioned GbUNE12 Genes negatively regulate cotton's resistance to Verticillium wilt, specifically by reducing or inhibiting... GbUNE12 Gene expression or activity to enhance resistance to Verticillium wilt in cotton, the aforementioned GbUNE12 The coding sequence of the gene is shown in SEQ ID NO:1, and its encoded amino acid sequence is shown in SEQ ID NO:2. This invention is the first to identify transcription factors in the whole genome of sea island cotton and discover the bHLH transcription factor gene with a 66 bp structural variation in its promoter region. GbUNE12 ,based on GbUNE12 Molecular markers developed for gene research can effectively distinguish between marine and terrestrial orthologous genes and can be used for marker-assisted screening of disease-resistant germplasm.

The invention discloses a gossypium barbadense regulatory protein GbAGP6 as well as a coding gene and application thereof. The invention belongs to the field of botany, and particularly relates to a gossypium barbadense regulatory protein GbAGP6 as well as a coding gene and application thereof. The substance for regulating and controlling the expression of the coding gene of the protein GbAGP6 or the substance for regulating and controlling the activity or content of the protein can be applied to any one of the following aspects: 1) regulating and controlling the disease resistance and plant height of plants; 2) preparing a product for regulating the disease resistance and plant height of plants; 3) cultivating plants with changed disease resistance and plant height; the expression of GbAGP6 in susceptible sea island cotton is obviously higher than that of GbAGP6 in disease-resistant sea island cotton, after the expression of GbAGP6 in the susceptible sea island cotton is reduced by using a virus-induced gene silencing technology, the morbidity of the sea island cotton is obviously reduced, the plant height is also obviously increased, and the disease-resistant variety level is achieved.