33 results about "Gossypium barbadense" patented technology

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the salt tolerance of island cotton and application of the SNP molecular marker. The site of the SNP molecular marker is located at the 45674375th site of a D02 chromosome of gossypium barbadense, and the allele at the 45674375th site is A or G. According to the invention, whole genome association analysis is carried out on the relative plant height and the relative overground part fresh weight in salt stress and salt-free stress environments, the SNP site related to the salt tolerance of the sea island cotton is obtained through screening, and the SNP molecular marker is accurate and efficient in detection and convenient and stable in amplification, can be used for molecular marker-assisted selection, and can be used for detecting the salt tolerance of the sea island cotton. The identification efficiency of different salt-tolerant cotton varieties is improved.

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

The invention discloses an SWEET15A01 gene promoter and application of Indel variation of the SWEET15A01 gene promoter in improvement of cottonseed oil content, the promoter has multiple variations between low-oil upland cotton Hubei cotton 22 (E22) and high-oil sea island cotton 3-79, resulting in differential expression of the gene in the later stage of ovule development. A transgenic material created by driving the gene expression by using a gossypium barbadense genotype promoter significantly increases the oil content of the seeds, and has no significant influence on fiber yield and quality related traits. A molecular marker is developed aiming at Indel variation of 21bp, genetic typing can be carried out on gossypium barbadense and upland cotton populations, and high-oil cotton germplasm and low-oil cotton germplasm can be distinguished. The molecular marker provided by the invention has important application value in the aspects of cotton molecular marker-assisted breeding and high-oil germplasm innovation.

The application discloses a sea island cotton chromosome fragment capable of improving fiber strength of upland cotton and application of the sea island cotton chromosome fragment. The application provides a chromosome fragment from sea island cotton, which is a fragment located from InDel molecular marker C6STR-3 to InDel molecular marker C6STR-10 on D06 chromosome of sea island cotton. The nucleotide sequence of the InDel molecular marker C6STR-3 is shown as SEQ ID NO. 3. The nucleotide sequence of the InDel molecular marker C6STR-10 is shown as SEQ ID NO. 6. The sea island cotton chromosome fragment provided by the application can significantly increase fiber strength of upland cotton when applied to cotton molecular breeding. Meanwhile, the InDel molecular marker developed by the application provides a molecular basis for breeding of high-fiber-strength cotton varieties.

This invention provides the application of the GbWAK5 gene in regulating the salt tolerance of sea island cotton, belonging to the field of plant genetic engineering technology. The nucleotide sequence of the GbWAK5 gene described in this invention is shown in SEQ ID NO.1, and the amino acid sequence of the protein encoded by the GbWAK5 gene is shown in SEQ ID NO.2. VIGS experiments have demonstrated that the GbWAK5 gene participates in the salt tolerance of sea island cotton by regulating ion homeostasis, and sea island cotton containing or highly expressing the GbWAK5 gene exhibits better salt tolerance. These results provide important information for understanding the members of the sea island cotton WAK gene family and provide potential target genes for further breeding of salt-tolerant varieties.

The invention discloses a sea island cotton SNP (Single Nucleotide Polymorphism) molecular marker and application thereof, and relates to the technical field of cotton molecular breeding, and the key points of the technical scheme are as follows: the SNP molecular marker is GbMA03G0171SNP; the nucleotide sequence of the GbMA03G0171SNP molecular marker is as shown in SEQ ID No.1, the polymorphic site of the GbMA03G0171SNP molecular marker is located at the 2195th site of the sequence as shown in SEQ ID No.1, and the polymorphism of the GbMA03G0171SNP molecular marker is A / T.

The invention discloses a transcription factor MYB59 gene molecular marker related to cotton verticillium wilt resistance and application of the transcription factor MYB59 gene molecular marker. In sea island cotton Hai7124, homologous sequences of regions of upstream 232 bp, GbMYB59 gene and downstream 25073 bp of the transcription factor GbMYB59 gene are inverted in the upland cotton TM-1, so that the promoter sequence of the upland cotton GhMYB59 gene is deleted in the TM-1, and the gene function is lost. Two haplotypes exist in cotton in a genome inversion region, and the promoter sequence of the haplotype 2 after the upstream 232 bp of the MYB59 gene is deleted compared with that of the haplotype 1. Compared with the haplotype 2, the haplotype 1 has a remarkable advantage in the aspect of improving the verticillium wilt resistance of the cotton. The haplotype 1 and the SV marker thereof have important effects and application prospects in improvement of verticillium wilt resistance of cotton and cultivation of new varieties of cotton with high verticillium wilt resistance.

The invention relates to application of a GbPIF3 gene functional structure domain in plant type regulation, which is characterized in that a plant type related gene Gb-PIF3 is obtained by cloning from an apical meristem in a hybrid F1 of sea island cotton-upland cotton, and the functional structure domain GbPIL of the gene is subjected to heterologous expression in arabidopsis thaliana through a transgenic technology. The photoresponse test and seedling property observation on the obtained transgenic material prove that the gene functional structure domain has the function in plant type regulation and control, can be used for transforming the plant type structure of upland cotton and regulating and controlling the flowering time, and has important significance on the improvement of the yield and quality of cotton, especially the genetic modification of close-planting cotton.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker GbarD031878030 for identifying fusarium wilt resistance of gossypium barbadense and application of the SNP molecular marker GbarD031878030. The invention belongs to the technical field of biology, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker for identifying gossypium barbadense wilt resistance and application of the SNP molecular marker. The invention provides a method for identifying or assisting in identifying sea island cotton wilt resistance, which comprises the following steps: detecting the genotype of an SNP (Single Nucleotide Polymorphism) site in a sea island cotton genome to be detected, and identifying or assisting in identifying the sea island cotton wilt resistance according to the genotype, the SNP site is located in a sea island cotton D03 chromosome, the nucleotide variety of the SNP site in a coding sequence is T or C, and the nucleotide variety of the SNP site in the coding sequence is T or C; the nucleotide is the 388th nucleotide of the sequence 1 in the sequence table. The SNP molecular marker disclosed by the invention can be used for rapidly and efficiently identifying the variety of the fusarium wilt-resistant sea island cotton in an early stage, assists in molecular assisted breeding of the sea island cotton, and has great significance in increasing the proportion of the sea island cotton in the whole textile industry and increasing the international influence of the sea island cotton in the whole textile industry.

The application provides a SNP molecular marker related to fiber length of Gossypium barbadense, and an amplification primer and application thereof, and belongs to the technical field of molecular markers. The SNP molecular marker related to fiber length of Gossypium barbadense is a C / T polymorphic site at the 124th base of a DNA fragment with a nucleotide sequence as shown in SEQ ID NO:1. The SNP molecular marker can be used for polymerization breeding and parent screening of fiber length of Gossypium barbadense, lays a foundation for molecular marker assisted selection and precise breeding of fiber length, and promotes production of Gossypium barbadense in Xinjiang.

The invention discloses an SNP molecular marker for identifying the number of fruit branches of gossypium barbadense and application of the SNP molecular marker. The invention belongs to the technical field of biology, and particularly relates to an SNP molecular marker for identifying the number of fruit branches of gossypium barbadense and application of the SNP molecular marker. The invention provides a method for identifying or assisting in identifying the number of fruit branches of gossypium barbadense, which comprises the following steps: detecting the genotype of an SNP (Single Nucleotide Polymorphism) site in a genome of gossypium barbadense to be detected, and identifying or assisting in identifying the number of fruit branches of gossypium barbadense according to the genotype, the SNP site is an SNP site on a chromosome D07 of gossypium barbadense, the nucleotide type of the SNP site is C or T, and the SNP site is the 811th nucleotide of a sequence 1 in a sequence table. The SNP molecular marker significantly associated with the number of the fruit branches of the sea island cotton can be used for sea island cotton yield trait molecule-assisted breeding, accelerates high yield, is suitable for early-stage efficient screening of machine-harvested cotton varieties, and improves the production potential of the sea island cotton.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying an initial fruit branch knot position of gossypium barbadense and application of the SNP molecular marker. The invention belongs to the field of nucleic acid detection, and particularly relates to an SNP molecular marker for identifying an initial fruit branch knot position of gossypium barbadense and application of the SNP molecular marker. The invention provides a method for identifying or assisting in identifying an initial fruit branch knot position of gossypium barbadense, which comprises the following steps: detecting the genotype of an SNP (Single Nucleotide Polymorphism) site in a gossypium barbadense genome to be detected, and identifying or assisting in identifying the initial fruit branch knot position of gossypium barbadense according to the genotype, the SNP site is an SNP site on a gossypium barbadense D07 chromosome, and the nucleotide type is C or T; the nucleotide is the 811th nucleotide of the sequence 1 in the sequence table. The SNP molecular marker obviously related to the beginning fruit branch knot position of the sea island cotton can be used for sea island cotton early-maturing character molecule auxiliary breeding, high-efficiency screening of early-maturing cotton varieties is assisted, and the economic value of the sea island cotton is improved.

The invention discloses a sea island cotton fiber length regulation protein GbIDM1 as well as a coding gene and application thereof. The invention belongs to the field of botany, and particularly relates to a sea island cotton fiber length regulation protein GbIDM1 as well as a coding gene and application thereof. The protein GbIDM1 or a substance for regulating and controlling the expression of a coding gene of the protein or a substance for regulating and controlling the activity or content of the protein can be applied to any one of the following aspects: 1) regulating and controlling the length of plant fibers; 2) preparing a product for regulating and controlling the length of the plant fiber; the method comprises the following steps: 1) preparing sea island cotton, 2) preparing sea island cotton, 3) culturing a plant with changed fiber length, 4) preparing a product for culturing the plant with changed fiber length, and 5) breeding the plant. The expression of GbIDM1 in the sea island cotton with short fiber is higher than that in the sea island cotton with long fiber, and after the expression of GbIDM1 in the sea island cotton with short fiber and the sea island cotton with long fiber is respectively reduced by using a virus-induced gene silencing technology, the fiber length of the sea island cotton is obviously increased.

The invention discloses an application of a cotton GbCG1 gene in improvement of plant genetic transformation efficiency. Relates to the technical field of transgenosis and provides specific application. According to the invention, a somatic embryo regeneration candidate gene GbCG1 is screened out. The gene is only expressed in sea island cotton, overexpression of GbCG1 in upland cotton can significantly improve the callus differentiation efficiency and shorten the somatic embryo generation period, only 4-5 months are needed from transformation to seedling emergence, the time is 3-5 months faster than that of a control group, and the transformation efficiency is 3.5 times that of the control group. The invention discloses application of a GbCG1 gene in promoting somatic embryogenesis of cotton and improving the gene transformation efficiency, and the callus differentiation efficiency can be remarkably improved by overexpressing the GbCG1 gene. And valuable gene resources are provided for genetic breeding. The method can effectively solve the problem of cotton transformation genotype limitation, and broadens the variety types of cotton genetic transformation receptors.

The application relates to a cotton fiber strength major gene NTL9 BS41 and application thereof. Through cotton transgenic technology, NTL9 BS41 is successfully introduced into Gossypium hirsutum and high expression of the gene in cotton fibers is realized, the fiber strength of cotton is significantly enhanced without negative influence on other fiber qualities, overexpression of NTL9 BS41 can effectively enhance the density and uniformity of cellulose microfibril accumulation, and the structure and performance of cotton fibers are significantly optimized. In addition, the gene is derived from Gossypium barbadense, compared with Gossypium hirsutum, there is an insertion type variation of 24 bp nucleotides in the CDS of the gene, and a molecular marker capable of effectively distinguishing Gossypium hirsutum and Gossypium barbadense haplotypes is successfully developed. The application provides new gene resources, technical means and germplasm resources for cotton fiber quality improvement, and has far-reaching application value and potential for promoting the progress of cotton breeding work.

The invention discloses application of protein GbCET2 and a coding gene thereof in regulating and controlling the number of fruit branches of gossypium barbadense. The invention belongs to the field of botany, and particularly relates to application of protein GbCET2 and a coding gene thereof in regulating and controlling the number of fruit branches of gossypium barbadense. The protein GbCET2 or a substance for regulating and controlling the expression of a coding gene of the protein or a substance for regulating and controlling the activity or content of the protein can be applied to any one of the following aspects: 1) regulating and controlling the number of fruit branches of plants; the method comprises the following steps of (1) preparing GbCET2 in sea island cotton, (2) preparing a product for regulating and controlling the number of fruit branches of the plant, (3) cultivating the plant with the changed number of fruit branches, (4) preparing a product for cultivating the plant with the changed number of fruit branches, and (5) breeding the plant, and after the expression of GbCET2 is reduced in the sea island cotton by utilizing a virus-induced gene silencing technology, the number of the fruit branches of the sea island cotton is remarkably increased.

The invention discloses a KASP molecular marker related to gossypium barbadense oil content and application, and relates to the technical field of molecular breeding, the key points of the technical scheme are that the molecular marker is at least one of the following: A / G mutation at the 51156847 base on a D07 chromosome; and / or C / T mutation at a 51250107 basic group on the chromosome D07; and / or G / C mutation at a 51248778 basic group on the D07 chromosome.

The application provides a cotton homozygous type rapid breeding method based on a created marker, and comprises the following steps: step one, using breeding material with a functional marker trait as a donor parent, and crossing the donor parent with an upland cotton with a target excellent trait, and continuously performing multiple generations of backcrossing and sister crossing to create a batch of core marker germplasm which gradually penetrates excellent genes of the upland cotton and stably carries the functional marker trait. The application uses the functional marker trait as a morphological index to realize rapid and nondestructive identification of the target trait in the early generation of cotton breeding, breaks through the technical bottleneck that the target trait is difficult to be accurately identified in the early stage in traditional backcross breeding, and improves the selection efficiency. The core marker germplasm created through multiple generations of backcrossing and sister crossing retains the excellent genes of the upland cotton, and stably carries the functional marker trait, so that the genetic marker provides reliable morphological selection basis for subsequent hybridization breeding, and avoids the problem of genetic background confusion caused by repeated backcrossing.

The invention discloses a pair of non-synonymous SNPs molecular markers for identifying sea island cotton wilt resistance on a D03 chromosome and application of the non-synonymous SNPs molecular markers. The invention belongs to the technical field of biology, and particularly relates to an SNPs molecular marker for identifying gossypium barbadense wilt resistance and application of the SNPs molecular marker. The invention provides a method for identifying or assisting in identifying sea island cotton wilt resistance, which comprises the following steps: detecting genotypes of SNPs sites in a sea island cotton genome to be detected, and identifying or assisting in identifying the sea island cotton wilt resistance according to the genotypes, the SNPs sites are a pair of non-synonymous SNPs sites on a sea island cotton D03 chromosome, and the SNPs sites are a pair of non-synonymous SNPs sites on a sea island cotton D03 chromosome. The nucleotides are located at the 67th site and the 105th site of the sequence 1 in the sequence table. The SNPs molecular marker disclosed by the invention can be used for molecular assisted breeding of disease-resistant sea island cotton, is beneficial to solving the problem of insufficient disease resistance of sea island cotton varieties in current cotton breeding, and also has great significance in overall improvement of quality and yield of sea island cotton.

This invention discloses a sea island cotton GbUNE12 The application of genes in regulating cotton resistance to Verticillium wilt, the aforementioned GbUNE12 Genes negatively regulate cotton's resistance to Verticillium wilt, specifically by reducing or inhibiting... GbUNE12 Gene expression or activity to enhance resistance to Verticillium wilt in cotton, the aforementioned GbUNE12 The coding sequence of the gene is shown in SEQ ID NO:1, and its encoded amino acid sequence is shown in SEQ ID NO:2. This invention is the first to identify transcription factors in the whole genome of sea island cotton and discover the bHLH transcription factor gene with a 66 bp structural variation in its promoter region. GbUNE12 ,based on GbUNE12 Molecular markers developed for gene research can effectively distinguish between marine and terrestrial orthologous genes and can be used for marker-assisted screening of disease-resistant germplasm.

The invention provides a high-temperature-resistant SNP (Single Nucleotide Polymorphism) molecular marker located at a GbarD0134580356 site of Gossypium barbadense and application of the high-temperature-resistant SNP molecular marker, and belongs to the technical field of cotton breeding, the physical position of the molecular marker is a 54580356 site basic group of a chromosome D01 of a Gossypium barbadense genome, and the deoxynucleotide polymorphism of the basic group at the site is G or A. The invention further provides a preparation method of the high-temperature-resistant SNP molecular marker. When deoxynucleotide of the SNP site is G, the sea island cotton is high-temperature-resistant sea island cotton, rapid identification of the high-temperature resistance of the sea island cotton germplasm can be realized by utilizing the SNP molecular marker, whether the sea island cotton germplasm to be detected has the high-temperature resistance or not can be accurately judged, and the method is convenient and efficient and provides a technical basis and support for cotton breeding.

The invention discloses a gossypium barbadense regulatory protein GbAGP6 as well as a coding gene and application thereof. The invention belongs to the field of botany, and particularly relates to a gossypium barbadense regulatory protein GbAGP6 as well as a coding gene and application thereof. The substance for regulating and controlling the expression of the coding gene of the protein GbAGP6 or the substance for regulating and controlling the activity or content of the protein can be applied to any one of the following aspects: 1) regulating and controlling the disease resistance and plant height of plants; 2) preparing a product for regulating the disease resistance and plant height of plants; 3) cultivating plants with changed disease resistance and plant height; the expression of GbAGP6 in susceptible sea island cotton is obviously higher than that of GbAGP6 in disease-resistant sea island cotton, after the expression of GbAGP6 in the susceptible sea island cotton is reduced by using a virus-induced gene silencing technology, the morbidity of the sea island cotton is obviously reduced, the plant height is also obviously increased, and the disease-resistant variety level is achieved.

The invention provides a method for carrying out chemical emasculation by utilizing glyphosate potassium salt based on sea island cotton, which comprises the following steps of: adopting random block design, taking a glyphosate-resistant sea island cotton strain as a test material, and carrying out stem and leaf spraying on different block cotton by utilizing glyphosate potassium salt solutions with different concentration gradients in the early stage of budding; the method comprises the following steps: observing the phenotype of abortion of a sea island cotton plant treated by glyphosate potassium salt, carrying out cytological observation, detecting the activity of pollen grains, and counting the boll forming number in a harvesting period through a hybridization test, so as to screen out the optimal glyphosate potassium salt concentration capable of enabling the sea island cotton to be aborted. Therefore, the feasibility of preparing hybrids through chemical emasculation of the gossypium barbadense by utilizing the glyphosate potassium salt is identified. According to the method, the key period of glyphosate-induced male abortion of the gossypium barbadense and the appropriate glyphosate emasculation concentration can be determined, and the boll setting rate is higher than 80%, so that it is feasible that the gossypium barbadense is subjected to chemical emasculation by utilizing glyphosate to prepare the hybrid.

The application discloses a Gossypium barbadense L. GbCYP72A2 gene, a coding protein thereof and application. The gene has a transcription start site at the 1st base from the 5' end, and a transcription termination codon at the 1558-1560th base. The coding frame is 1557 bases, and the gene codes 519 amino acids. The gene has 97% similarity with a homologous gene GbCYP72A1, but 3% sequence difference can endow a plant with higher resistance to verticillium wilt. The gene can enrich disease-resistant gene resources, provide an effective new tool for cotton breeding against verticillium wilt, and the overexpression of the gene can reduce the sensitivity of a plant to ABA in the germination stage. The down-regulated expression of the gene can also promote the growth of the plant.

The invention discloses a molecular marker related to sea island cotton fiber quality and application thereof, and relates to the technical field of sea island cotton breeding, the key points of the technical scheme are as follows: the molecular marker is at least one of InDel-203, InDel-86 and InDel-423, the polymorphism of the molecular marker is a deletion / insertion sequence, and the molecular marker is at least one of InDel-203, InDel-86 and InDel-423. The molecular marker InDel-203 is obtained through amplification of a specific primer pair as shown in a nucleotide sequence SEQ NO.1-2, the molecular marker InDel-86 is obtained through amplification of a specific primer pair as shown in a nucleotide sequence SEQ NO.3-4, and the molecular marker InDel-423 is obtained through amplification of a specific primer pair as shown in a nucleotide sequence SEQ NO.5-6.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying the fruit branch type of gossypium barbadense and application of the SNP molecular marker. The invention belongs to the technical field of biology, and particularly relates to an SNP molecular marker for identifying the fruit branch type of gossypium barbadense and application of the SNP molecular marker. The invention provides a method for identifying or assisting in identifying the fruit branch type of island cotton, which comprises the following steps: detecting the genotype of an SNP (Single Nucleotide Polymorphism) site in the genome of island cotton to be detected, and identifying or assisting in identifying the fruit branch type of island cotton according to the genotype, the SNP site is an SNP site on the D07 chromosome of island cotton, and the nucleotide type is C or T; the nucleotide is the 811th nucleotide of the sequence 1 in the sequence table. The SNP molecular marker obviously related to the sea island cotton fruit branch type can be used for sea island cotton mechanical harvesting character molecule auxiliary breeding, is beneficial to efficient breeding of mechanical harvesting varieties, and promotes the mechanical harvesting process of sea island cotton.

The invention discloses application of a cotton GbGELP21A gene in plant disease resistance, and belongs to the field of gene engineering. According to the invention, a GbGELP21A protein in a GDSL lipase family is identified in gossypium barbadense, the amino acid sequence of the GbGELP21A protein is as shown in SEQ ID NO.2, and the nucleotide sequence of the coding gene GbGELP21A of the GbGELP21A protein is as shown in SEQ ID NO.1. An overexpression vector and a silent vector of the gene are constructed, arabidopsis thaliana and cotton are transformed, and transgenic experiment results show that after being infected by verticillium wilt, the condition of a GbGELP21A overexpression plant is obviously superior to that of a wild type plant, the condition of a silent plant is more serious, and the GbGELP21A gene is capable of regulating the disease resistance of the plant. The invention lays a foundation for verticillium wilt resistance breeding, and has great economic benefits and wide application prospects for plant production.

The application discloses application of a GbTCP20 gene in improving cotton Verticillium wilt resistance and belongs to the technical field of biotechnology.The GbTCP20 gene disclosed by the application encodes a TCP transcription factor.The application provides a full-length ORF nucleotide sequence and an amino acid sequence of the GbTCP20 in a Gossypium hirsutum var. hirsutum L. variety Hai7124.The expression of the gene is significantly up-regulated by a Verticillium albo-atrum Eulge et Hunter induction.Silencing of the GbTCP20 expression in cotton weakens the Verticillium wilt resistance.Overexpression of the cotton GbTCP20 in Arabidopsis thaliana enhances the Verticillium wilt resistance.The cotton GbTCP20 positively regulates lignin biosynthesis, and the combination of molecular biology technology and plant physiology experiments reveals that the GbTCP20 positively regulates lignin biosynthesis to endow the cotton with Verticillium wilt resistance.

The application discloses application of a VdR4 gene in improving cotton verticillium wilt resistance and belongs to the field of biotechnology application.The VdR4 gene disclosed by the application encodes beta,1,3-glucanase.The application provides a full-length ORF nucleotide sequence and an amino acid sequence of VdR4 in a heterologous tetraploid island cotton material H7124.Cotton VdR4 interacts with an effector protein PeVD1 of a verticillium wilt fungus and participates in cotton verticillium wilt resistance.VdR4 is significantly up-regulated in expression in cotton roots induced by the verticillium wilt fungus.Overexpression of the gene in cotton can significantly improve the disease resistance of plants.

The invention discloses an application of an AOP-like gene in improving the disease resistance of cotton verticillium wilt, and belongs to the field of biotechnology application. The AOP-like gene provided by the invention is used for coding AOP protease. The invention provides a full-length ORF nucleotide sequence and an amino acid sequence of AOP-like in a sea island cotton genetic standard line Hai7124 and an upland cotton genetic standard line TM-1. The gene is induced by verticillium dahlia to realize obvious up-regulation expression. Silent AOP-like in cotton enhances the resistance to verticillium wilt. The research of the invention provides a new cotton verticillium wilt resistance thought.