48 results about "Gossypium barbadense" patented technology

The invention relates to the field of molecular breeding, and particularly relates to a fiber strength related molecular mark from gossypium barbadense and application thereof. The molecular mark is HAU0883125 and TMB1125220, wherein the HAU0883125 is closely linked with the site qFS-C14-1 of cotton fiber strength QTL, and the TMB1125220 is closely linked with the site qFS-C20-1 of cotton fiber strength QTL; and the qFS-C14-1 is located on chromosome C14, and the qFS-C20-1 is located on chromosome C20. The molecular mark can be used for improving the selection efficiency of fiber strength, overcoming the defect in existing breeding technology for fiber quality identification and accelerating the culturing progress of high-quality new varieties.

The invention relates to a molecular breeding method for improving cotton fiber lengths, fiber strengths, and micronaire values. The method is applied in oriented improving of crop (cotton) agronomic properties, and in selective breeding of novel breeds. According to the invention, cotton fiber quality detection is carried out upon gossypium barbadense chromosome segment introgression lines (CSIL), and an introgression line IL080-D6-1 with substantially increased fiber length and fiber strength, and substantially reduced micronaire value is selected. The introgression line is regarded as a non-backcross parent, and Xinluzao No.38, which is a breed from Xinjiang cotton region, is regarded as a backcross parent. Crossing is carried out between the two. The resulting breeds are backcrossed for 2 generations and self for 3 generations. With molecular marker auxiliary selection, a novel cotton line with good fiber length, fiber strength, and micronaire value is obtained. With the method, anovel high-quality and high-yield cotton line, which is Nannong gossypium barbadense No.2, is obtained.

The invention relates to a cotton GbSTK gene and encoding protein thereof. Sea island cotton Pima90-53 is taken as a material, a cDNA-AFLP technology is used for screening to obtain differential fragments related to verticillium wilt resistance, and then, a reverse transcription-polymerase chain reaction (RT-PCR) and a RACE (rapid-amplification of cDNA ends) technology are used for amplifying to obtain a resistance gene of cotton verticillium wilt, namely a serine / threonine protein kinase (GbSTK) gene. The functional studies of the gene and the encoding protein thereof show that the GbSTK gene has certain effect on resisting verticillium wilt.

The invention discloses a molecular breeding method capable of improving length of cotton fibers by using gossypium barbadense chromosome segment introgression line, which is particularly used for crop (cotton) economical character oriented improvement and new variety bredding. An introgression line IL040-A4-1 of which the fiber length is increased obviously is screened out by cotton fiber detection of the gossypium barbadense chromosome segment introgression line. In the method, introgression line is used as a nonrecurrent parent, Xinluzao No.26, which is a variety promoted in cotton area innorth of Xinjiang, is used as a recurrent parent are hybridized, hybridizing, two generations of back crossing and three generations of selfing are performed, molecular markers are also used to help to select cotton new lines with high fiber length. A high-quality and high-yield cotton new line named 'Nannong Haidao No.1' was bred by using the method.

The invention discloses a high-yield cultivation method of crop rotation of sea island cottons with long and short fruit branches, which comprises the following steps of: autumn tillage and winter irrigation, fine land preparation, preparation of suitable varieties, field planting mode, scientific field management and timely harvesting; according to the method, the long fruit branch sea island cotton and the short fruit branch sea island cotton are planted in an intercropping way, so that the marginal effect of the long fruit branch sea island cotton can be increased, the utilization rate of space resources is effectively improved, and the economic benefit is greatly improved; the method mainly adopts the currently commonly used planting mode of machine-picked cotton in Xinjiang, one filmcorresponds four rows, one film of long fruit branch loose plant type sea island cotton is adjacent with two films of short fruit branch compact plant type sea island cotton and one film of long fruitbranch sea island cotton, the arrangement is repeated in this way, the arrangement of crops in the field is optimized, the planting layout of the plant type structure alternate arrangement of loose-compact-loose is integrally formed, no significant difference exists in the quality of the sea island cotton fiber, but the yield of seed cotton and lint cotton can be increased by 10.07% and 10.95% respectively, and the yield and benefit of cotton per unit area can be obviously improved.

The invention belongs to the technical field of plant genetic engineering and particularly relates to cell wall extensin gene GbEXPATR separated and cloned from cotton and application. The cell wall extensin gene GbEXPATR which is obtained through sea island cotton fiber specific expression in a cDNA library at different sea island cotton fiber development stages and lack of a second structural domain, and a nucleotide sequence is represented by SEQ ID NO:1, wherein 52-306 represents a basic group region which is a coding region and exceptionally and efficiently expressed in a sea island cotton fiber elongation period, a conversion period and a secondary wall synthesis initial stage. An obtained overall-length ORF is established on a plant excessive expression vector Pcambia2301M, sea island cotton is converted into upland cotton by using an agrobacterium-mediated transformation genetic transformation method, the quality of transgenic offspring fiber is analyzed, and the cell wall extensin gene GbEXPATR can effectively promote fiber elongation is proved. A Micron-aire reduced. The fiber quality is improved from the grade C2 to the grade B2. The breakage ratio and the strength are improved. The cotton fiber quality can be effectively improved by utilizing the cell wall extensin gene GbEXPATR.

The invention relates to the technical field of molecular breeding, in particular to a molecular marker related to fiber length from Sea Island Cotton Sea 1 and its application. The molecular marker is CGR6381 150 、HAU0734 210 and CGR5151 140 . The invention helps to overcome the deficiency of fiber length identification in the existing breeding technology, improves the selection efficiency of fiber length, and accelerates the cultivation process of high-quality new varieties.

The invention relates to a cotton stearyl desaturase gene GbSSI2 and application thereof. The invention belongs to the technical field of plant genetic engineering and particularly relates to the isolation, cloning, functional verification and application of the gene GbSSI2 identified in gossypium barbadense. The cDNA sequence of the gene GbSSI2 is shown as SEQ ID NO:1; the amino acid sequence of the protein of the encoding gene of the gene GbSSI2 is shown as SEQ ID NO:2. The functional verification shows that the gene GbSSI2 has the functions of regulating and controlling the synthesis of relevant disease-resistant endogenous hormones in cotton, such as salicylic acid and jasmonic acid, and can be applied to the cultivation of the anti-verticillium dahliea cotton strains through genetic transformation.

The invention relates to a method that utilizing the cotyledon stalk of Gossypium barbadense( G. barbadense L. ) as explant to produce regenerative plants by tissue culture. The seeds of Gossypium barbadense disinfected are laid on the culture medium of seed to germinate, and the cut cotyledon stalks are laid in the inducing culture medium, and grown clump buds are cut to lay in the stretching culture medium for stretching, afterwards transferred into culture medium for growing root, to get regenerative plants. The inventive method can produce tufted buds that clumping and have no limitation of genetic types between every kind, short period of cultivation, normal growth of regenerative plants. The regenerative system of tufted buds of Gossypium barbadense cotyledon stalks established by said inventive method is suitable for every kind of Gossypium barbadense.

The invention belongs to the technical field of cotton molecule breeding, particularly relating to a method for building a cotton fiber transcription genetic linkage map by EST-SSR signs. The method comprises the following steps: taking gossypium barbadense Pima 3-79 as a male parent and Gossypium hirsutum Emian 22 as a female parent, and hybridizing to obtain F1; planting F1, and causing F1 to be inbreeded to obtain F2; taking the F2 single plant as a starting material for the fiber transcription map to be plotted; extracting the RNA of the fiber of the field F2 single plant in 5 days after the single plant blooms, and carrying out reverse transcription of the RNA into cDNA to be served as a plotting group of the transcription map; utilizing the reported EST-SSR primer and the primer shown by a self-designed sequence table SEQ ID NO:1-90 to carry out the plotting group analysis to the F2 single plant by a denatured polyacrylamide gel electrophoretic analysis; and finally, analyzing data by MAPMAKER / EXP.3.0 mapping software, and manufacturing and obtaining the transcription map. Compared with the existing method, the method of the invention is simple and practical, has accurate QTL positioning and is convenient for cloning genes relevant to cotton fiber development.