The invention belongs to the technical field of
spectral analysis, and discloses a
plant cell wall
microfibril orientation polarization Raman spectrum measurement method, which comprises: 1, carrying out polarization Raman spectrum
data acquisition on a sample to be measured; step 2, acquiring required data based on a
data acquisition result of the polarization Raman spectrum; 3, performing nonlinear parameter fitting on the required data, obtaining an optimal parameter combination and a corresponding coefficient through a minimum
error function, and performing forced symmetry post-
processing on the optimal Raman
tensor matrix; step 4, calculating a
microfilament angle of a corresponding angle according to the symmetrical
tensor parameter; in the fourth step, based on the symmetrical
tensor matrix, the angle needed for rotating the tensor matrix to the main axis direction is calculated; and carrying out normalization
processing on the calculated angle value. The technical problems that in the prior art, due to the fact that a nonlinear
fitting algorithm is complex and prone to being affected by
noise and experimental errors, the fitting convergence effect is poor, and the stability and reliability of a detection result are insufficient are solved.