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Modified bacterial cellulose

a technology of bacterial cellulose and modified cellulose, which is applied in the direction of sugar derivates, organic chemistry, chemistry apparatus and processes, etc., can solve the problems of not being able to produce modified bacterial cellulose, and achieve the effect of improving the modula of young peopl

Inactive Publication Date: 2005-09-06
CP KELCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0006]An object of the invention is to develop a bacterial cellulose, wherein the major axis (width) of ribbon-shaped microfibril is varied, and various properties, especially Young's modulas are improved.
[0007]The inventors investigated in order to achieve the above object, and found that a modified bacterial cellulose wherein ribbon-shaped microfibrils are varied can be obtained by adding a cell division inhibitor or an organic reducing agent to a culture medium which induces variation of the shape of cellulose - producing bacteria, and that properties, especially Young's modulus, are improved compared with conventional bacterial cellulose.

Problems solved by technology

However it has not been reported to produce modified bacterial cellulose by changing the form of cellulose-producing bacteria artificially to vary ribbon-shaped microfibrils.

Method used

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Examples

Experimental program
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example 1

[0047]The culture medium used was composed of 50.0 g / l sucrose, 5.0 g / l “Total Amino Acid” (Ajinomoto Co., Inc.), 0.2 g / l phytic acid, 2.4 g / l magnesium sulfate and 1.0 g / l ammonium sulfate (pH 5.0).

[0048]Seed culture was carried out by placing 20 ml of the above culture medium in a 100 ml flask with baffle, inoculating Acetobacter pasteurianus FERM BP-4176, and then culturing at 25° C. for 3 days with stirring at 200 rpm. The culture medium was crushed by a blender, and added to a main culture medium having the above composition in a concentration of 2% seed culture.

[0049]The main culture was carried out by static culture at 25° C. During the culture, culture solution and bacterial cellulose were withdrawn, and the morphology of bacteria was observed by an optical microscope, an electron microscope and an atomic force microscope.

[0050]Six main culture media were used, and nalidixic acid (NA) was added thereto in a concentration of 0.01 mM, 0.05 mM, 0.1 mM, 0.2 mM or 1.0 mM except o...

example 2

[0058]Acetobacter pasteurianus FERM BP-4176 was cultured in static culture, and the culture solution and bacterial cellulose were withdrawn, and the shape of bacteria was observed by the optical microscope, the electron microscope and the atomic force microscope, similar to Example 1, except that chloramphenicol was used instead of nalidixic acid.

[0059]That is, six main culture media having the aforementioned composition were used, and chloramphenicol (CP) was added thereto in a concentration of 0.1 mM, 0.2 mM, 0.3 mM, 0.5 mM or 1.0 mM except one medium to which CP was not added.

[0060]As a result, the length of the cellulose-producing bacterium increased with increasing the CP concentration up to 8 to 12 times as long as the bacteria cultured in no CP medium.

[0061]As an example, the shape of bacterium cultured in the 0.3 mM CP medium for 2 days taken by the optical microscope (×1000), and shown in FIG. 4, and that cultured in no CP medium for 2 days is shown in FIG. 5.

[0062]The CP r...

example 3

[0066]Acetobacter pasteurianus FERM BP-4176 was cultured in static culture, and the culture solution and bacterial cellulose were withdrawn, and the form of bacteria was observed by the optical microscope, the electron microscope and the atomic force microscope, similar to Example 1, except that chloramphenicol was used instead of nalidixic acid.

[0067]That is, four main culture media having the aforementioned composition were used, and dithiothreitol (DTT) was added thereto in a concentration of 0.5 mM, 1.0 mM or 2.0 mM except one medium to which DTT was not added.

[0068]As a result, the length of the cellulose-producing bacterium decreased with increasing the DTT concentration.

[0069]As an example, the shape of bacterium cultured in the 1.0 mM DTT medium for 2 days taken by the optical microscope, and shown in FIG. 7. As can be seen from the photograph, the length of the bacterium cultured in 1.0 mM DTT medium wat shortened to ⅓ to ½ of the bacteria cultured in no DTT medium.

[0070]Th...

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Abstract

This invention provides a bacterial cellulose comprising ribbon-shaped microfibrils having a thickness of 10 to 100 nm and a width of 160 to 1000 nm or a bacterial cellulose comprising ribbon-shaped microfibrils having a thickness of 10 to 100 nm and a width of 50 to 70 nm. The former bacterial cellulose can be produced by culturing cellulose-producing bacteria in a culture medium containing a cell division inhibitor, and the latter can be produced by culturing the bacterium in a culture medium containing an organic reducing agent. The bacterial cellulose is modified from conventional bacterial cellulose in the major axis, and is improved in Young's modulus, etc.This invention provides a bacterial cellulose comprising ribbon-shaped microfibrils having a thickness of 1 to 9 nm and a width of 160 to 1000 nm or a bacterial cellulose comprising ribbon-shaped microfibrils having a thickness of 1 to 9 nm and a width of 50 to 70 nm. The former bacterial cellulose can be produced by culturing cellulose-producing bacteria in a culture medium containing a cell division inhibitor, and the latter can be produced by culturing the bacterium in a culture medium containing an organic reducing agent. The bacterial cellulose is modified from conventional bacterial cellulose in the major axis, and is improved in Young's modulus, etc. <?insert-end id="INS-S-00001" ?>

Description

BACKGROUND OF THE INVENTION[0001]This invention relates to bacterial cellulose (BC) of which ribbon-shaped microfibrils are artificially modified to improve Young's modulus and a method of producing the same.[0002]The bacterial cellulose can be used as various industrial materials, clothing materials, materials for medical supplies, functional materials, materials for foods and so on.[0003]It is known that Acetobacter xylinum ATCC 23769 produces a mat-shaped cellulose which can be used for medical pads (Japanese Patent KOKAI 59-120159). It is also known that Acetobacter aceti subsp. xylinum ATCC 10821, etc. produce bacterial cellulose composed of ribbon-shaped microfibrils (U.S. Pat. No. 4,742,164). The size of the ribbon-shaped microfibrils is said to be 20 to 50 nm (Ed. by Tokyo Techno. Forum Secretariat, “Jinrui to Bio (Humanity and Bio)”, P329, 1993 Yomiuri Nippon Television (enter) which may be measured without discrimination of the major axis (width and the minor axis (thickne...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12P19/00
CPCC12P19/04
Inventor ISHIHARA, MASARUYAMANAKA, SHIGERU
Owner CP KELCO
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