Molecular marker tightly linked to kiwi fruit total acid content trait QTL locus and application of molecular marker

Abstract

The invention discloses a molecular marker tightly linked to a kiwi fruit total acid content trait QTL locus and application of the molecular marker. As for a primer designed according to the molecular marker, a forward primer TA-F is 5'-GTAAATGCATGGTAAAGAGGTCC-3', and a reverse primer TA-R is 5'-AGGAGAAAGGGAAAATAAAACGC-3'. As for the molecular marker, a major QTL locus for controlling total acid in a kiwi fruit is located for the first time, 21.75 percent phenotype variance can be explained, and the total acid content of the kiwi fruit can be forecasted by detecting the molecular marker relevant to the total acid content, so that superior individual plants with higher total acid content are accurately and rapidly screened.

Description

technical field

[0001] The invention belongs to the technical field of molecular biology and genetic breeding, and more specifically relates to a molecular marker closely linked to a QTL site of kiwifruit total acid content traits and its application. Background technique

[0002] Kiwi fruit is rich in vitamin C, dietary fiber and various mineral nutrients, and is a healthy fruit favored by consumers. China is the original origin center of the genus Actinidia. Actinidia is extremely rich in resources and has high genetic diversity. China has about 96% of the world's total resources of Actinidia species. Relying on abundant natural resources, breeding new varieties with excellent traits plays a key role in the healthy and stable development of the kiwifruit industry. The main acid components of kiwi fruit are quinic acid and citric acid, and also contain a small amount of malic acid. The content of sugar and acid in fruit and their ratio have a very important influence on t...