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Molecular marker for breeding bright-peel tomato varieties and application of molecular marker

A molecular marker and tomato technology, which can be used in applications, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of vitamin and lycopene loss, achieve bright appearance, save production costs, and "good slag removal" Effect

Inactive Publication Date: 2020-09-04
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The processing of conventional preserved tomato or dried fruit needs to go through processes such as washing, blanching, peeling, hardening, sugar infiltration, drying or drying, etc., to remove the abundant water in the fruit and promote the infiltration of sugar, but in the peeling process, Causes loss of vitamins and lycopene

Method used

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  • Molecular marker for breeding bright-peel tomato varieties and application of molecular marker
  • Molecular marker for breeding bright-peel tomato varieties and application of molecular marker
  • Molecular marker for breeding bright-peel tomato varieties and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The development of molecular markers for the breeding of bright peel or dried and preserved tomato varieties, the specific steps are as follows:

[0034] S1, with glossy as the female parent and tomato wild germplasm LA1375 as the male parent, was crossed to obtain the F1 generation; the F1 generation plants were self-pollinated to obtain the F2 generation.

[0035] S2, 563 F2 generation plants were sown and colonized in plastic greenhouses. Under normal management, the phenotype can be basically judged 2-3 weeks after the fruit sets, and the phenotype is obvious and stable during the green ripening period. Investigate more than 3 fruits per plant. The glossy mutant fruit is bright in color and feels sticky when touched, while the normal fruit is matte in color and smooth to the touch. If more than 3 fruits are all bright and sticky, the glossy phenotype is judged to be recessive, otherwise it is dominant.

[0036] In the F2 population, there are 138 individual plants...

Embodiment 2

[0056] Using the molecular marker GLS and primer sequences obtained in Example 1, 13 parts of plant materials obtained by glossy mutants as donor materials were identified, and compared with their fruit phenotypes, the specific steps were as follows:

[0057] (1) Using the genomic DNA of related tomato strains as a template, the primers are GLS, and the reaction system and program recommended by Easy Taq polymerase of Beijing Quanshijin Biotechnology Co., Ltd. are used for amplification, and the PCR products are amplified with 2% agarose gel Electrophoretic detection.

[0058] Wherein, the PCR amplification reaction system described in the above technical scheme, according to the total volume of 15 μl, includes the following components:

[0059]

[0060] The PCR amplification reaction conditions were: pre-denaturation at 94.0°C for 3 minutes; denaturation at 94.0°C for 30 s, annealing at 55.0°C for 30 s, extension at 72.0°C for 30 s, and 33 cycles; extension at 72.0°C for 5...

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Abstract

The invention relates to the field of tomato molecular marker breeding, in particular to a molecular marker for breeding bright-peel tomato varieties and an application of the molecular marker. Tomatospontaneous mutant glossy and wild tomato LA1375 are used to construct an F2 segregation population, the glossy gene is located within 600 kb of chromosome 1 by BSR positioning strategy and linkage analysis, and the molecular marker GLS closely linked with the glossy gene is obtained. Tomato varieties are bred by the molecular marker and a prime of the molecular marker, resources are provided fordevelopment of special tomato varieties, and a foundation is laid for fruit development biology research.

Description

technical field [0001] The invention belongs to the field of tomato breeding, and in particular relates to a molecular marker for bright peel tomato variety breeding and its application. Background technique [0002] The tomato epidermis is the cuticle, which is the first barrier for plant self-protection and plays an important role in the growth and development of plants. The cuticle is the interaction interface between plants and the environment. It can seal plant cells and reduce plants from biotic and abiotic stress, such as reducing water loss other than stomatal transpiration, reducing pathogen infection and insect feeding. The cuticle can also effectively block mechanical damage. The polysaccharides in the cuticle can affect the compactness and firmness of the plant cuticle, which in turn affects the tension and density of the plant's outer skin. Tomato skin can not only affect the resistance and storability of the fruit, but also greatly affect the sensory quality a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/04
CPCA01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 欧阳波周玉红高虎于会洋陈蓉石春美卢永恩叶志彪
Owner HUAZHONG AGRI UNIV
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