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SNP (Single Nucleotide Polymorphism) site related to green-keeping property of Chinese cabbage and application of SNP site

A Chinese cabbage, site technology, applied in the field of SNP sites, can solve problems such as yellowing loss of bulbous leaves

Active Publication Date: 2022-03-11
SHENYANG AGRI UNIV
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Problems solved by technology

In addition, during the storage and transportation of the bulbs after harvest, the yellowing of the bulbs will also cause a lot of loss

Method used

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  • SNP (Single Nucleotide Polymorphism) site related to green-keeping property of Chinese cabbage and application of SNP site
  • SNP (Single Nucleotide Polymorphism) site related to green-keeping property of Chinese cabbage and application of SNP site
  • SNP (Single Nucleotide Polymorphism) site related to green-keeping property of Chinese cabbage and application of SNP site

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Experimental program
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Embodiment 1

[0061] Embodiment 1. Development and application of SNP molecular markers related to green traits

[0062] 1. Design of SNP molecular markers

[0063] Using the Chinese cabbage DH line 'FT' as the test material, a stable green-sustaining mutant cl was created by adopting the EMS mutagenesis treatment method of germinating seeds. Through genome resequencing of wild-type 'FT' and mutant cl, a SNP site associated with greenness of Chinese cabbage was screened. Through the amplification of the competitive allele-specific primers, it is possible to identify whether greenness remains and whether it is homozygous, and can be used for auxiliary selection of greenness-holding breeding materials. details as follows:

[0064] F 1 F 2 Genome detection was carried out, and a SNP site 0513 related to the green trait was found in Chinese cabbage, which was the 24th position of sequence 1 in the sequence table, and the genotype of the SNP site 0513 was CC, TT or CT.

[0065] According to...

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Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) site related to the green-keeping property of Chinese cabbage and application of the SNP site. The invention provides an application of a substance for detecting the genotype of the SNP site 0513 in identifying whether to-be-detected Chinese cabbage has the persistent green property or not. The SNP site 0513 is the 24th site of the sequence 1 in the sequence table. According to the invention, the SNP marker linked with the persistent green property is developed in the Chinese cabbage, whether the material is persistent green and homozygous or not can be identified by amplifying the specific primer of the competitive allele, the SNP marker can be used for auxiliary selection of persistent green breeding materials, and the problems that the conventional breeding cycle is long and is easily influenced by the environment can be effectively solved.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a SNP site related to the greenness of Chinese cabbage and an application thereof. Background technique [0002] Plant leaf color is the comprehensive expression of various pigments in the leaves. In normal leaves, chlorophyll is dominant, usually appearing as green. Leaf senescence is the final stage of plant leaf development and an important link in the recycling of plant nutrients (Parket al., 2007). The most obvious sign of leaf senescence is the green fading caused by the degradation of chlorophyll and the subsequent coloration of anthocyanins or flavonoids (Matile et al., 1999). Stay-green refers to the characteristic that the chlorophyll of the aging leaves of plants does not degrade or degrades insignificantly, and remains green for a long time or even does not turn yellow at all (Kusaba et al., 2013). Due to its remarkable characteristics, the green-sustaining variant h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2563/107C12Q2531/113
Inventor 王楠冯辉纪淑娟
Owner SHENYANG AGRI UNIV
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