Simple screening method for wheat gene producing unreduced gamete

A screening method and unreduced technology, applied in genetic breeding and biological fields, can solve problems such as difficulty in screening wheat genotypes, cumbersome screening process, and difficulty in distant hybridization, so as to promote practical application efficiency, avoid separation, and improve breeding efficiency Effect

Active Publication Date: 2010-12-08
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the distant relationship between the parents, such distant hybridization is difficult, and it is possible to obtain hybrid seeds only through techniques such as immature embryo rescue.
Therefore, such a screening process is cumbersome and has a large workload, and it is very difficult to screen out the desired wheat genotype from a huge wheat population.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Transferring the meiotic recombination gene of tetraploid wheat landrace AS2255 into hexaploid wheat SHW-L1:

[0026] We crossed the Chinese tetraploid wheat local variety AS2255 (Triticum turgidum ssp.turgidum) with Triticum turgidum AS60 to obtain hybrid seeds F 1 . these f 1 F obtained after seed germination 1 The hybrid plants have 21 chromosomes and chromosome sets ABD (14 from the AB chromosome set of tetraploid wheat AS2255, and 7 from the D chromosome set of A. japonicum AS60). In theory, such F 1 Chromosomes of hybrid plants randomly segregate during meiosis, and the possibility of obtaining male and female gametes with 21 chromosomes at the same time is extremely small, so the frequency of gametes with normal vitality is very low, so such F 1 Plants selfing usually do not set fruit. However, we found F from AS2255 and AS60 in Year 1 1 48 seeds were obtained from 238 florets self-crossed, and the seed setting rate was as high as 20.17%. In the ...

Embodiment 2

[0032] Embodiment 2: Wheat SHW-L1 with meiotic recombination gene and Aegilops variabilis distant hybridization:

[0033] According to the normal method of crossing two common wheat varieties, that is, without using special methods such as tissue culture, we used SHW-L1 as the female parent and Aegilops variabilis AS24 as the male parent in 2003 and 2005, and obtained 30.68% and 48.37% respectively. % hybrid seed setting rate (Table 2), shows that the hybrid combination is easy to obtain hybrids. At the same time, we crossed Aegilops variabilis AS24 as the female parent and SHW-L1 as the male parent in 2003 and 2005 respectively, and obtained 9.09% and 9.62% hybrid seed setting rates respectively. See Table 2 for details, indicating that backcrossing can also be successful. The germination experiment of these hybrids showed that the hybrid seeds obtained by the reciprocal crossing combination of SHW-L1 and Aegilops variabilis AS24 were easy to germinate.

[0034] Table 2. Hyb...

Embodiment 3

[0036] Embodiment 3: Wheat that does not have meiotic recombination gene is self-crossed with Aegilops variabilis distant hybrid almost not fruiting:

[0037] We used the popular wheat varieties Chuanyu 16 and Chuanmai 28 without meiotic recombination genes, the new wheat line TKL1, the wheat local varieties Xinzhongchang, Kaixian Luohanmai, Zhongguochun and the ph1b mutant etc. to cross distantly with Aegilops variabilis , all showed ideal hybridability, and were easy to obtain hybrids (Table 3), indicating that there was no hybridization barrier in the distant hybridization between wheat and Aegilops variabilis.

[0038] Table 3. The fruit setting of crosses between wheat and Aegilops variabilis

[0039] parent material

[0040] The genome of wheat is AABBDD (2n=6x=42), the genome of Aegilops variabilis is UUS 1 S 1 (2n=4x=28), their hybrid F 1 Plants usually have 35 chromosomes and their chromosome set is ABDUS 1 , that is, by A, B, D, U, S 1 A haploid consis...

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PUM

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Abstract

A simple method for screening the wheat genotype able to generate unreduced gamete for breaking through the 'reduplicative bottle neck' of reduplicating monoploid includes such steps as distant hybridizing between wheat and Aegilops variabilis to obtain plant F1, selfing, and screening.

Description

technical field [0001] The invention relates to the fields of genetic breeding and biotechnology, in particular to a simple screening method for wheat genotypes that produce unreduced gametes in a haploid state. Background technique [0002] Usually, plant somatic cells contain two sets of chromosomes, which come from male and female gametes respectively, so the number of chromosomes is doubled (2n). Haploid (haploid) is a plant body that has gamete chromosome composition and only contains half (n) of the normal somatic cell chromosome number. Common wheat (the chromosome set is AABBDD, 2n=6x=42) is an allohexaploid (2n=6x=42) composed of three chromosome sets A, B, and D, and each chromosome set has 7 pairs of chromosomes. The A and B genomes of wheat are provided by the tetraploid wheat (Triticum turgidum L., the genome is AABB, 2n=28), while the D genome is provided by the diploid Aegia (some called rough goat grass, Aegilops tauschii Cosson Or Triticum tauschii (Cosson...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/04A01H1/02A01G7/00
Inventor 刘登才张连全颜泽洪郑有良
Owner SICHUAN AGRI UNIV
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