Method for increasing inductivity and direct planting percent of Chinese cabbage and green Chinese cabbage hybrid microspore-derived embryo

Abstract

The invention discloses a method for increasing the inductivity and direct planting percent of Chinese cabbage and green Chinese cabbage hybrid free microspore culture embryoid and aims to solve the problems of low inductivity and planting percent and long culture period of the microspore embryoid in the prior art. In the method, the inductivity of the embryoid is increased by 6.2 times by using an NLN culture medium to which 40 mu m anti-auxin, namely, 2-(4-chlorophenoxy) isobutyric acid and 130 g.L<-1> cane sugar are added and the direct planting percent is up to 34.00 percent by transferring the embryoid to a hormone-free MS culture medium when the embryoid is mature at the 21st day after inoculation. In the method, only about 40 days are needed from the inoculation of a microspore to the acquisition of a regeneration plant, the embryoid which does not growth to a plant grows to a plant after primary secondary culture and the final planting percent is up to 80 percent. The method of the invention has the advantages of greatly saving time, improving the efficiency of microspore culture, accelerating a breeding process, lowering high cost caused by secondary culture, reducing thepollution to valuable materials and laying a basis for rapid and mass creation of high-quality breeding materials, along with simpleness and practicability.

Description

technical field [0001] The invention relates to a method for culturing microspores of Chinese cabbage and pakchoi hybrids, is a plant cell culture technology, and belongs to the field of biotechnology. Background technique [0002] Both Chinese cabbage and pakchoi belong to the AA genome (2n=20) vegetables of the genus Brassica. They are biennial plants with leaves as the main edible organ. The effect of fire is widely planted in the south and north of our country, and occupies an important position in the vegetable supply of urban and rural residents. Chinese cabbage and Chinese cabbage have significant heterosis. In order to exert the heterosis, the parents used for seed production must be highly homozygous genetically. The method of culturing free microspores can quickly obtain excellent inbred lines and self-incompatible lines, which can be directly used to breed new varieties or indirectly used as excellent parents for variety improvement, greatly shortening the breedi...

AI Technical Summary

Problems solved by technology

In 2002, my country's Sun Mengxiang inserted mature microspore embryos and immature embryos into MS medium containing calcium chloride and ammonium nitrate, and the seedling rate of embryoid bodies could reach 85% and 80% respectively, but this method did not improve the microspore growth rate. The embryo induction rate cannot meet the requirements of high-efficiency culture system

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