Seed breeding method of naturally-capped dwarf compact cabbage-type rape
A rapeseed, compact technology, applied in the field of rapeseed breeding, can solve the problem of rapeseed with limited knots, and achieve the effect of improving planting benefits and high efficiency
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[0019] Example 1: Breeding experiment of main inflorescence with natural topping, limited knot angle, and dwarf compact rapeseed
[0020] (1) Hybridization: The female parent of Dwarf Brassica napus 3H004 and the compact rapeseed 7399-8 were used as the male parent to obtain the hybrid F 1 . Parents and hybrid F 1 Planted in the field and transplanted in the greenhouse after vernalization outdoors.
[0021] (2) set up double haploid (DH) line group: to the F that step (1) obtains 1 For the plant, the pollen of the young buds on the main inflorescence and the upper branch inflorescence of the plant was taken at the initial flowering stage for microspore culture, and EMS was used for mutagenesis and colchicine for chromosome doubling to obtain 446 double haploid (DH) line populations, Specific steps are as follows:
[0022] 1) Mutation and isolation of rapeseed microspores: at the initial flowering stage of rapeseed, hybrid F 1 The flower buds with a length of 3.0-4.0mm on ...
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