Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture method for improving embryonic birth rate of cabbage stalk

A technology for cabbage and cultivation methods, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve problems such as affecting the rate of embryo production, unsatisfactory cultivation effect, affecting the quality of embryos, etc., so as to improve the utilization efficiency. Effect

Inactive Publication Date: 2012-07-18
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS +1
View PDF3 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Subsequently, Yan Zhun et al. (1999,), Tang Qinglin et al. (2000), Liu Yanling et al. (2006), Yuan Suxia (2009) and others also reported, but the efficiency of microspore embryogenesis in cabbage cabbage is generally not high. In terms of species, the cultivation effect is very unsatisfactory
A large number of studies on the culture of free microspores of the genus Brassica have shown that the genotype of the donor plant is extremely important to the microspore embryogenesis, which not only affects the embryo production rate, but also affects the quality of the embryo (Chuong et al., 1988) , limiting the further application of microspore culture technology in the genetic breeding and basic research of cabbage
Although people have done some research work on improving the factors affecting the embryo emergence rate of free microspores of cabbage, the embryo emergence rate of free microspores of cabbage is still not high, and it is difficult to obtain embryoid bodies in some genotypes ( Fang Shugui et al., 2005a, 2005b; Liu Yanling et al., 2006; Lu Ruiju et al., 2006), seriously hampered the practical application of free microspore culture technology, therefore, it is necessary to find a simple and effective way to improve the embryo emergence of cabbage difficult-to-embryo genotype varieties. The rate method has important practical significance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) In this example, the head cabbage and rape inflorescences were picked from the experimental field of Zhenjiang Institute of Agricultural Sciences, and the varieties are not limited.

[0025] (2) Culture medium preparation: culture medium including different stages of microspore culture, its components and the weight of each component contained in each liter of medium are:

[0026] B5 washing medium: B5 liquid medium 1L + sucrose 30 g / L, pH 6.0, high temperature and high pressure sterilization; wherein, B5 liquid medium, in 1L, consists of: NaH 2 PO 4 2H 2 O 169.5mg, KNO 3 2500mg, (NH 4 ) 2 SO 4 134mg, MgSO 4 ·7H 2 O 500mg, MnSO 4 4H 2 O 10mg, H 3 BO 3 3mg, ZnSO 4 ·7H 2 O 2mg, KI 0.75mg, Na 2 MoO 4 2H 2 O 0.25mg, CuSO 4 ·5H 2 O 0.025mg, CoCl 2 ·6H 2 O 0.025mg, Na 2 -EDTA 37.3mg, FeSO 4 ·7H 2 O 27.8mg, CaCl 2 .2H 2 O 150mg, VB 1 10mg, VB 6 1mg, VPP 1mg, inositol 100mg and the rest in distilled water.

[0027] NLN-13 induction medium: N...

Embodiment 2

[0046] The difference between the medium in this example and the medium in Example 1 is as follows, B5 liquid medium 1L + sucrose 30g / L, pH 6.0, high temperature and high pressure sterilization; NLN-13 induction medium: NLN-13 liquid medium 1L + sucrose 130g / L, pH 6.1, filter sterilized; embryoid solid differentiation medium: B5 medium + sucrose 20g / L, agar 11 g / L, pH 6.0, high temperature and high pressure sterilization; rooting medium: MS medium + White sugar 20g / L, agar 7g / L, pH 5.9, high temperature and high pressure sterilization;

[0047] The present embodiment cultivates head cabbage as follows:

[0048] Step 1, picking healthy, no pests and diseases, flower buds from late mononucleate to early binucleate on the inflorescence of cabbage and rape inflorescence as donor plants for microspore culture, the petal and anther length ratio of cabbage inflorescence is 1.2, rapeseed The petal and anther length ratio of the inflorescence is 0.75, the two kinds of flower buds are ...

Embodiment 3

[0060] The difference between the medium in this example and the medium in Example 1 is as follows: B5 liquid medium 1L + sucrose 30g / L, pH6.0, high temperature and high pressure sterilization; NLN-13 medium: NLN-13 liquid medium 1L + sucrose 130g / L, pH 6.1, filter sterilized; embryoid body solid differentiation medium: B5 medium + sucrose 20g / L, agar 12 g / L, pH 6.0, high temperature and high pressure sterilization; rooting medium: MS medium + white sugar 20g / L, agar 7g / L, pH 5.9, high temperature and high pressure sterilization;

[0061] The present embodiment cultivates head cabbage as follows:

[0062] Step 1, pick the flower buds that grow healthy on the head cabbage inflorescence and rapeseed, no damage by diseases and insect pests, late mononucleate to early binucleate as the donor plant for microspore culture, the petal and anther length ratio of the head cabbage inflorescence is 1.1, rapeseed The petal and anther length ratio of the inflorescence is 0.75. The two kin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a culture method for improving embryonic birth rate of crops and particularly relates to a culture method for improving the embryonic birth rate of cabbage stalk, belonging to the technical field of plant culture. According to the culture method disclosed by the invention, a microspore culture technology is utilized for co-culture of the cabbage stalk and rapeseed microspores, so that the germ extraction rate of the cabbage stalk can be significantly improved and the problem of low embryogenesis frequency of microspore culture of the cabbage stalk is further solved.

Description

technical field [0001] The invention relates to a cultivation method for increasing the embryonic birth rate of crops, in particular to a cultivation method for increasing the embryonic birthrate of head cabbage, belonging to the technical field of plant cultivation. Background technique [0002] Cabbage (Brassica oleracea L. var. Capitata) is a plant of Brassica genus Brassica, which is a variety of Brassica oleracea L. Also known as cabbage, cabbage, cabbage, etc. Heading cabbage has the characteristics of cold resistance, disease resistance, strong adaptability, easy storage and transportation, high yield, and good quality. It is widely cultivated in various parts of my country and occupies an important position in vegetable cultivation and supply. Plays a big role in the annual supply of vegetables. And a large amount of it is exported to foreign countries, and it also occupies a very important position in the vegetable export trade. At present, most of the main cabba...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 张振超潘跃平戴忠良姚悦梅秦文斌肖燕潘永飞
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com