Culture method for improving embryonic birth rate of cabbage stalk

A technology for cabbage and cultivation methods, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve problems such as affecting the rate of embryo production, unsatisfactory cultivation effect, affecting the quality of embryos, etc., so as to improve the utilization efficiency. Effect

Inactive Publication Date: 2012-07-18
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Subsequently, Yan Zhun et al. (1999,), Tang Qinglin et al. (2000), Liu Yanling et al. (2006), Yuan Suxia (2009) and others also reported, but the efficiency of microspore embryogenesis in cabbage cabbage is generally not high. In terms of species, the cultivation effect is very unsatisfactory
A large number of studies on the culture of free microspores of the genus Brassica have shown that the genotype of the donor plant is extremely important to the microspore embryogenesis, which not only affects the embryo production rate, but also affects the quality of the embryo (Chuong et al., 1988) , limiting the further application of microspore culture technology in the genetic br

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) In this example, the head cabbage and rape inflorescences were picked from the experimental field of Zhenjiang Institute of Agricultural Sciences, and the varieties are not limited.

[0025] (2) Culture medium preparation: culture medium including different stages of microspore culture, its components and the weight of each component contained in each liter of medium are:

[0026] B5 washing medium: B5 liquid medium 1L + sucrose 30 g / L, pH 6.0, high temperature and high pressure sterilization; wherein, B5 liquid medium, in 1L, consists of: NaH 2 PO 4 2H 2 O 169.5mg, KNO 3 2500mg, (NH 4 ) 2 SO 4 134mg, MgSO 4 ·7H 2 O 500mg, MnSO 4 4H 2 O 10mg, H 3 BO 3 3mg, ZnSO 4 ·7H 2 O 2mg, KI 0.75mg, Na 2 MoO 4 2H 2 O 0.25mg, CuSO 4 ·5H 2 O 0.025mg, CoCl 2 ·6H 2 O 0.025mg, Na 2 -EDTA 37.3mg, FeSO 4 ·7H 2 O 27.8mg, CaCl 2 .2H 2 O 150mg, VB 1 10mg, VB 6 1mg, VPP 1mg, inositol 100mg and the rest in distilled water.

[0027] NLN-13 induction medium: N...

Embodiment 2

[0046] The difference between the medium in this example and the medium in Example 1 is as follows, B5 liquid medium 1L + sucrose 30g / L, pH 6.0, high temperature and high pressure sterilization; NLN-13 induction medium: NLN-13 liquid medium 1L + sucrose 130g / L, pH 6.1, filter sterilized; embryoid solid differentiation medium: B5 medium + sucrose 20g / L, agar 11 g / L, pH 6.0, high temperature and high pressure sterilization; rooting medium: MS medium + White sugar 20g / L, agar 7g / L, pH 5.9, high temperature and high pressure sterilization;

[0047] The present embodiment cultivates head cabbage as follows:

[0048] Step 1, picking healthy, no pests and diseases, flower buds from late mononucleate to early binucleate on the inflorescence of cabbage and rape inflorescence as donor plants for microspore culture, the petal and anther length ratio of cabbage inflorescence is 1.2, rapeseed The petal and anther length ratio of the inflorescence is 0.75, the two kinds of flower buds are ...

Embodiment 3

[0060] The difference between the medium in this example and the medium in Example 1 is as follows: B5 liquid medium 1L + sucrose 30g / L, pH6.0, high temperature and high pressure sterilization; NLN-13 medium: NLN-13 liquid medium 1L + sucrose 130g / L, pH 6.1, filter sterilized; embryoid body solid differentiation medium: B5 medium + sucrose 20g / L, agar 12 g / L, pH 6.0, high temperature and high pressure sterilization; rooting medium: MS medium + white sugar 20g / L, agar 7g / L, pH 5.9, high temperature and high pressure sterilization;

[0061] The present embodiment cultivates head cabbage as follows:

[0062] Step 1, pick the flower buds that grow healthy on the head cabbage inflorescence and rapeseed, no damage by diseases and insect pests, late mononucleate to early binucleate as the donor plant for microspore culture, the petal and anther length ratio of the head cabbage inflorescence is 1.1, rapeseed The petal and anther length ratio of the inflorescence is 0.75. The two kin...

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PUM

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Abstract

The invention relates to a culture method for improving embryonic birth rate of crops and particularly relates to a culture method for improving the embryonic birth rate of cabbage stalk, belonging to the technical field of plant culture. According to the culture method disclosed by the invention, a microspore culture technology is utilized for co-culture of the cabbage stalk and rapeseed microspores, so that the germ extraction rate of the cabbage stalk can be significantly improved and the problem of low embryogenesis frequency of microspore culture of the cabbage stalk is further solved.

Description

technical field [0001] The invention relates to a cultivation method for increasing the embryonic birth rate of crops, in particular to a cultivation method for increasing the embryonic birthrate of head cabbage, belonging to the technical field of plant cultivation. Background technique [0002] Cabbage (Brassica oleracea L. var. Capitata) is a plant of Brassica genus Brassica, which is a variety of Brassica oleracea L. Also known as cabbage, cabbage, cabbage, etc. Heading cabbage has the characteristics of cold resistance, disease resistance, strong adaptability, easy storage and transportation, high yield, and good quality. It is widely cultivated in various parts of my country and occupies an important position in vegetable cultivation and supply. Plays a big role in the annual supply of vegetables. And a large amount of it is exported to foreign countries, and it also occupies a very important position in the vegetable export trade. At present, most of the main cabba...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张振超潘跃平戴忠良姚悦梅秦文斌肖燕潘永飞
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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