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Method for obtaining chilli embryoid by microspore induction

A culture method and microspore technology are applied in the field of cell engineering to achieve the effect of simple steps and high efficiency

Inactive Publication Date: 2006-09-27
NORTHWEST A & F UNIV
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Problems solved by technology

[0005] Breeding parental inbred lines (pure lines) using microspore culture has been widely used in Chinese cabbage, cabbage and other cruciferous plants, but there is no report on the induction of a large number of pepper microspores to obtain embryoid bodies and then regenerate plants

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  • Method for obtaining chilli embryoid by microspore induction
  • Method for obtaining chilli embryoid by microspore induction
  • Method for obtaining chilli embryoid by microspore induction

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Embodiment Construction

[0035] According to the technical scheme of the present invention, the method for cultivating the embryoid body of capsicum microspores, the specific implementation steps are:

[0036] (1) Determining the test material: the first-generation hybrid pepper variety P51 with comprehensive and excellent traits was used as the test material.

[0037] (2) Select flower buds: at the early or full flowering stage of P51, through the acetic acid magenta tablet method [cited from Dudu  zkum cinek, Rukiye Tipirdamam. The effect of cold treatment and charcoal on the in vitro androgenesis of pepper (Capsicum annuum L.) .TurkJ Bot, 2002, 26: 131-139] Microscopic examination selects flower buds in the uninucleate marginal stage. The appearance and morphology of the flower buds are as long as the corolla and the calyx or slightly longer than the calyx. The diameter of the flower bud is 5 mm and the length is 7 mm. Periodically remove the blooms on the plant to keep it blooming.

[0038] (3) ...

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Abstract

The culture process of inducing chilli microspore to obtain embryoid belongs to the field of plant cell engineering. The process includes the steps of trialing material, selecting bud, pre-treating bud, preparing liquid culture medium and solid culture medium, sterilizing and packing liquid culture medium and solid culture medium, sterilizing bud, abacterial stripping of anther, inoculating anther, culturing anther, naturally separating microspore, forming embryoid, etc. The process can obtain microspore embryoid with capacity of growing normally in high seedling forming rate, and has a period of about 8 weeks. Applying the process in breeding variety can speed the breeding process, raise the selecting effect and speed the pure variety breeding.

Description

technical field [0001] The invention belongs to the field of cell engineering, and relates to a method for freeing and culturing capsicum microspores, in particular to a culturing method for inducing capsicum microspores to obtain embryoid bodies. Background technique [0002] Haploid plants can be obtained by microspore culture. Then, the double haploid (diploid pure line) can be obtained by doubling with colchicine, and the pure line can be directly obtained as a parent or intermediate material for breeding. Dissociative microspore culture is a method to obtain pure lines at the single-cell level. The obtained pure lines have uniform traits, strong intergenerational stability, and high homozygosity. [0003] Haploid has important theoretical significance and application value in genetics and breeding. The research and application of haploid breeding technology has greatly accelerated the process of breeding new varieties. If the haploid gametes ...

Claims

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Application Information

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IPC IPC(8): A01H1/00C12N5/04
Inventor 巩振辉张菊平刘珂珂黄炜李大伟
Owner NORTHWEST A & F UNIV
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