DNA sequence capable of regulating and controlling fertility of rice and application thereof

Abstract

The invention relates to the technical field of rice breeding, in particular to a DNA sequence capable of regulating and controlling the fertility of rice and an application thereof. The combination of a male sterile rice mitochondrial specific open reading frame and a mitochondrial leading peptide converts rice varieties and leads to sterility of a product without pollen nucleus. The method can quickly and efficiently obtain dominant genic male sterile materials of different rice varieties according to a transgenic method, and the sterile materials have pollen-free type abortion, are stable in sterility, and are not affected by temperature and environment. The DNA sequence provided by the invention can be widely used in rice genetic breeding, especially in directionally created pollen-free dominant genic male sterile materials, and can be widely used in dominant genic male sterile recurrent selection breeding.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a DNA sequence capable of regulating rice fertility and its application. Background technique [0002] There are many methods and technical routes used in rice genetic breeding, such as hybrid breeding, mutation breeding, molecular marker-assisted breeding, transgenic breeding, etc., and recurrent selective breeding as a very effective breeding method has been widely used in corn breeding. For the recurrent selection of self-bred crops, wheat has been practiced relatively successfully. Although some attempts have been made in rice, for example, many breeders have proposed to use Pingxiang dominant genic male sterility or photosensitive genic male sterility genes for rice recurrent selection breeding research. Some progress has been made in the recurrent selection breeding of restorer lines with wild sterility genes interacting with each other. But the effect is always not ...

AI Technical Summary

Problems solved by technology

The low-temperature-sensitive dominant genic sterile rice (gene TMS) of Sichuan Agricultural University is a temperature-sensitive male sterility, and the pollen abortion is mainly caused by attenuation and dyeing; Zhejiang University and China Rice Research Institute use gamma rays respectively Three dominant genital sterile materials were obtained from rice varieties, and the pollen abortion was mainly aborted and dyed, but there were problems such as incomplete abortion, a small amount of self-fertilization, and enlarged anther sacs that were difficult to distinguish; from According to the current reports, there are many rice dominant sterility materials to choose from, but due to the limitation of stability, there are probably not many of them that are really practical.

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