Molecular marker of sesame dominant genic male sterility gene and preparation method and application thereof

A male sterility gene and molecular marker technology, which can be used in biochemical equipment and methods, DNA/RNA fragments, and microbial determination/inspection, etc.

Inactive Publication Date: 2014-08-20
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems in the development of my country's sesame industry are: ①The output is often low and unstable, hovering at the level of 70 kg per mu for a long time (Yang Mei, Huang Fenghong. The status quo and existing problems, development trends and countermeasures of China's sesame industry. China Oil , 2009, 34(1):7-12); ② Poor disease resistance, prone to stem point blight and Fusarium wilt, and no immune or highly resistant materials have been found so far (Zhang X R, Zhao Y Z, Cheng Y, Feng X F, et al. Establishment of sesame germplasm core collection in China. Genetic Resources and Crop Evolution, 2000, 47: 273-279); ③ poor appearance quality, low oil content, weak market competitiveness
However, recurrent selection general

Method used

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  • Molecular marker of sesame dominant genic male sterility gene and preparation method and application thereof

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Effect test

Embodiment 1

[0041] A preparation method of sesame dominant nuclear male sterility gene molecular marker, the steps are:

[0042] 1. Obtaining the fragments related to dominant nuclear male sterility in sesame:

[0043] A. Experimental materials and population construction: In 2002, 5 wild sesame resources were introduced from India, one of which was "Yezhi 2" ( Sesamum malabaricum Burm, 2n=26) can grow normally and successfully hybridized with the cultivar "Ezhi 1". From a distant hybrid (Yezhi 2×Ezhi 1) F 1 Several plants with the highest sterility rate were selected by substitution and backcrossed with Ezhi No. 1, and this was repeated for 2 generations, and then the obtained BC 2 The best sterile plants in the population were selected for brother-sister crossing with the fertile plants, and the population with a sterile plant rate close to 50% was selected as the offspring for about 4 consecutive generations of sibling crossing, and a new sesame male sterile line W1098AB was bred. ...

Embodiment 2

[0060] A kind of application of the molecular marker of sesame dominant nuclear male sterility gene in the selection of sesame parents, the steps are:

[0061] A. Construction of sesame dominant nuclear male sterile segregation population: select the fertile and sterile strains in the dominant nuclear male sterile line of artificial bagging and hybridization for multiple generations, and then carry out brother-sister crossing to obtain F 0 generation seed, for F 1 The plants are covered with large mosquito nets for reproductive isolation planting to obtain fertile segregated populations.

[0062] B. Fertility identification: During the full-flowering period of sesame, the fertility identification of each single plant of the segregated group of siblings is carried out in the field. Since there is basically no difference between the sterile anther and the fertile anther of the sterile line with the naked eye, after pinching the anther It is difficult to determine whether eac...

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Abstract

The invention discloses a molecular marker of sesame dominant genic male sterility gene and a preparation method and application thereof. A forward primer sequence of the marker GB50 is ATGGGTTTATGGCAGGCT, and a reverse primer sequence is GGACTACTCCTCCTCCCCA; a forward primer sequence of SBM298 is CCCCTTTTCACTTACGTACAGCAG, and a reverse primer sequence is CTCTTCCTCCACCATCTCCTCTTC. The preparation method comprises the steps of: A, respectively numbering dominant genic male sterility brother-sister inbreeding segregation population in the seedling stage, collecting tender leaves from each individual plant, treating by liquid nitrogen and storing in a refrigerator for standby; B, conducting biosynthesis by using EST-SSR primers of SBM series; C, amplifying by a PCR procedure; D, conducting PCR amplification and product detection; E, acquiring a polymorphism marker; and F, acquiring a molecular marker associated with male sterility. The method can obtain 100% male sterility population, significantly improve the efficiency of sesame recurrent selection, and avoid the trouble of eradication of 50% fertile plants until the flowering phase by using morphological characteristics. The invention has obvious effect, low cost, no pollution and very broad application prospects.

Description

technical field [0001] The invention belongs to the field of sesame genetic breeding and molecular biology, and more specifically relates to a sesame dominant nuclear male sterility gene molecular marker, and also relates to a method for preparing a sesame dominant nuclear male sterility-related molecular marker, and also relates to a sesame dominant nuclear male sterility related molecular marker. The application of sesame dominant nuclear male sterility molecular markers in molecular marker-assisted selection to improve the efficiency of high-yield breeding using sesame dominant nuclear male sterility genes. Background technique [0002] Sesame is one of the main oil crops in my country. Sesame oil has pure aroma and high nutritional value, and is known as the "king of oils". The annual sesame planting area in my country is about 600,000 hectares, accounting for one-tenth of the world's total area, with a total output of 600,000-700,000 tons, accounting for a quarter of th...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 刘红艳赵应忠吴坤杨敏敏
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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