Construction method for high-density genetic map of scylla paramamosain

A genetic map and technology of Scylla simulacrum, which is applied in the field of construction of high-density genetic map of Scylla simulans, can solve the problem of difficulty in obtaining parents of all sibling families at the same time, restricting the construction of genetic maps, and immature artificial breeding and breeding technologies. and other problems, to achieve the effect of rich polymorphism, high density of genetic map and good quality.

Active Publication Date: 2019-03-19
SHANTOU UNIV
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Problems solved by technology

[0004] Due to the immature artificial breeding and reproduction technology of some species, or it is difficult to obtain the parents of all siblings at the same time, this limits the construction of genetic maps

Method used

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  • Construction method for high-density genetic map of scylla paramamosain
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  • Construction method for high-density genetic map of scylla paramamosain

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Embodiment 1

[0024] A method for constructing a high-density genetic map of blue crabs, mainly comprising the following steps:

[0025] 1. Mapping group construction

[0026] Select female crabs that have been mated, large in size, complete in body and limbs, full of ovary development, strong in vitality and free of parasites as parent crabs, and cultivate them in the breeding crab pool, and transfer them to the hatching pool to wait for hatching when they develop to brood. . After hatching, the larvae are transferred to the nursery pond for cultivation until the second stage of the larvae. The hatched parent crab samples were dissected to take the internal muscles of the large pincers, which were stored in a -80°C refrigerator for later use. Then select healthy juvenile crab seedlings at the second stage and transfer them to ponds for breeding. When they grow to 2.5 months old, 129 individuals are randomly selected. Male and female were determined according to the shape of the abdomen,...

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Abstract

The invention relates to a construction method for a high-density genetic map of scylla paramamosain. The construction method for the high-density genetic map of the scylla paramamosain comprises thefollowing four steps: constructing a mapping population, constructing a simplified genomic library and carrying out high-throughput sequencing, carrying out genetic typing and constructing a high-density genetic map. The genotype of a male parent is reversely ratiocinated by the genotype of a female parent and the genotype of an offspring, then genetic typing is carried out, therefore, the geneticmap is constructed, a new thinking and method are provided for construction of genetic maps of animal and plant families which only have single parent or difficultly have double parents simultaneously in a sample material, construction of the genetic map is facilitated, a research course of genetic background is pushed, and the construction method has good application prospect in the field of molecular breeding. A parting technology based on a SNP label is adopted, the construction method has the advantages of numerous labels, abundant polymorphism, genetic stability and the like, and the obtained genetic map is high in density and good in quality, and can be used for QTL positioning of follow-up important economic character and assisted assembly of genome.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a method for constructing a high-density genetic map of the mud crab. Background technique [0002] Scylla paramamosain is an important marine fishery resource and mariculture crab in my country. It has the characteristics of large size, fast growth, rich nutrition and delicious meat, and is very popular among people. In recent years, the artificial breeding output of blue crabs in my country has exceeded 140,000 tons year after year, and has maintained a good momentum of development, with great development space and breeding potential. With the continuous expansion of the breeding scale, the demand for improved blue crabs is also rising. However, so far, artificially cultivated seedlings mainly come from natural seedlings in sea areas. Due to the limitation of natural conditions in sea areas, it is difficult to guarantee their quality and quantity. In addi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888G16B40/00G16B20/20
CPCC12Q1/6888C12Q2600/156Y02A40/81
Inventor 马洪雨石西李升康章跃陵郑怀平刘文华方少彬
Owner SHANTOU UNIV
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