Method for efficiently developing sorghum sterile line
A sterile line and sorghum technology, applied in the field of sorghum genetics and breeding, can solve the problems of limited genetic diversity, inability to improve target traits, lack of genetic resources, etc. Effect
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[0021] Example 1 Breeding of compact sorghum maintainer lines and sterile lines
[0022] 1. Breeding of compact sorghum maintainer line (Btx623ERC4)
[0023] (1) Select about 500 plump, complete and healthy seeds of the purified sorghum maintainer line Btx623. Treat with EMS (ethyl methanesulfonate) at concentrations of 0.10%, 0.15%, 0.20%, 0.25% and 0.30% for 12 hours. After the treatment, the seeds were rinsed with tap water for 2 hours, and then sown in the experimental field of the Crop Institute of Shandong Academy of Agricultural Sciences. Before heading and flowering, each individual plant was bagged and self-crossed to obtain M 1 generation seeds.
[0024] (2) M to be harvested 1 The seeds of each individual plant are planted into panicle rows, about 200 rows, 10-30 trees in each row, and all individual plants in each panicle row are bagged and self-crossed to obtain M 2 generation seeds.
[0025] (3) will M 2 Mix the seeds of all individual plants in each ear ro...
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