Construction method and application of Haizi hybrid scallop restorer line

A method for constructing a restorer line, which is applied in the field of shellfish breeding technology--hybrid scallop commercial seedling cultivation, can solve the problems of inability to establish male sterile lines, maintainer lines and restorer lines, and achieves overcoming obstacles to self-fertilization, low cost effect

Active Publication Date: 2015-08-12
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in animal breeding, there have been no reports of male sterile and female fertile individuals at home and abroad so far, so it is impossible to establish male sterile lines, maintainer lines and restorer lines similar to crops for hybrid breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Breeding of Haizi hybrid scallop restorer line: Select 50 large individual scallops from the male sterile line of Haizi hybrid scallop, fertilize them with sperm from different purple scallops after promoting maturation and oviposition, and establish at least 20 backcross families At the same time, the purple scallops for sperm were self-propagated to establish an inbred family, and the growth and survival of the backcross offspring were measured, and the combining ability test was carried out; from the second year onwards, according to the results of the combining ability test and the development of the gonads, one of them with excellent traits and good traits was selected every year. For the backcross family containing hermaphroditic scallops, the seed shells of the related male sterile family will be continuously backcrossed with the sperm of the purple scallop hybrid family for sperm to establish a backcross family until all the backcross families are hermaphroditi...

Embodiment 2

[0024]At the end of March 2008, the bay scallops with mature gonads were taken, and the eggs of bay scallops were obtained after temperature stimulation. After insemination with the sperm of purple scallops, the first-generation seedlings of hybrids of bay scallops and purple scallops were obtained and raised. In the spring of 2009, the first-generation scallops of the above-mentioned hybrids were matured, and the scallops that were large and only developed in the female area were selected from them. After 30 minutes of drying in the shade, the scallops were placed in a container filled with seawater at 23°C to stimulate spawning. The sperm of the scallop was mixed and fertilized, and the backcross population of the sea purple hybrid scallop and the purple scallop was bred. Most of the backcross populations were hermaphroditic after maturation in the spring of 2010, but <10% of the individuals were still male sterile.

[0025] Continue to backcross the above-mentioned male ste...

Embodiment 3

[0029] In the spring of 2010, male sterile individuals in the first generation of hybrids of bay scallop eggs and purple scallop sperm established in 2009 were selected, stimulated to lay eggs after maturation, and fertilized with purple scallop sperm. Eight backcross families were established and raised. to make. After ripening in the spring of 2011, it was found that most of the individuals in all families were hermaphroditic, and a small number of individuals had agenesis of gonads, which proved that the recovery of Haizi hybrid scallops could be bred by backcrossing the male sterile individuals in the first generation of Haizi hybrids with purple scallops. Tie.

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Abstract

The invention relates to a construction method and application of an argopecten irradians-argopecten purpuratus hybrid scallop restorer line. The restorer line is argopecten purpuratus which can recover the male fertile characteristic for male sterile line progeny, wherein argopecten irradians-argopecten purpuratus hybrid scallops are hybrid progeny of argopecten irradians eggs and argopecten purpuratus sperms. The construction method includes the steps that excellent individuals in a male sterile line are selected, the different argopecten purpuratus sperms are used for continuous backcross until the progeny are all hermaphrodite, and the restorer line of the male sterile line is obtained; finally, the restorer line is hybridized with the corresponding male sterile line of the argopecten irradians-argopecten purpuratus hybrid scallops to produce argopecten irradians-argopecten purpuratus hybrid scallop commodity seeds. The restorer line of the male sterile line is set up in animals for the first time, the obstacle to self-fertilization in hybridization of hermaphrodite scallops is effectively overcome, and the yield of the produced argopecten irradians-argopecten purpuratus hybrid scallops in the current year is larger than that of argopecten irradians and is increased by over 50%.

Description

technical field [0001] The invention belongs to shellfish breeding technology-a method for cultivating commercial hybrid scallop seedlings, and in particular relates to a construction method and application of a restorer line of sea purple hybrid scallop (the hybrid offspring of bay scallop ova and purple scallop sperm). Background technique [0002] The bay scallop (Argopecten irradians irradians) is native to the Atlantic coast of the United States and was introduced to my country in 1982. The bay scallop grows fast, has a wide range of temperature adaptability, and has strong stress resistance. It is very suitable for farming in the northern seas of my country. At present, the output and farming area account for more than 50% of the scallop farming in my country. However, due to the long-term inbreeding after the introduction, the germplasm of bay scallops has been seriously degraded during the breeding process. The size of commercial shellfish is getting smaller and smal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王春德马斌孙奎府赵玉明刘博周顺王文琪赵侠苗国英
Owner QINGDAO AGRI UNIV
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