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Micro-satellite primer used for identifying Tridacna maxima and Tridacna rosewateri, and identification method

An identification method, the technology of Tridacna novae, applied in biochemical equipment and methods, measurement/inspection of microorganisms, DNA/RNA fragments, etc., can solve the problem that the morphological characteristics of giant clam and nova clam are difficult to distinguish and identify and other problems, to achieve the effect of not being affected by the environment and developmental period, the result is intuitive, and the method is simple

Active Publication Date: 2018-06-01
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

Because the morphological differences between the two kinds of giant clams are very small, it is often difficult to distinguish them solely by morphological characteristics; it is even more difficult to distinguish the larval giant clams and nova giant clams based on their morphological characteristics

Method used

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  • Micro-satellite primer used for identifying Tridacna maxima and Tridacna rosewateri, and identification method

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Effect test

Embodiment 1

[0021] see figure 1 , using sterilized tweezers and small scissors to cut a small amount of mantle tissue from the foot silk pores of Tridacna longis and Tridacna novae. Add 400 μl of lysate and 10 μl of proteinase K (10 mg / ml) to the centrifuge tube containing the mantle tissue, mix well on a shaker, and digest in a water bath at 55°C until the tissue is completely dissolved to obtain the first solution. Add an equal volume of saturated phenol (200μl), chloroform / isoamyl alcohol (24:1) (200μl) mixture and extract three times, centrifuge at 10000rpm for 10-15min each time, and absorb the supernatant with a pipette. Add 1 mL of pre-cooled absolute ethanol to the centrifuge tube containing the supernatant, and precipitate overnight. Finally, it was washed with 70% alcohol, dried at room temperature, and dissolved in 200 μL of ultrapure water to obtain genomic DNA. The concentration and purity of DNA were detected by ultraviolet spectrophotometer, and the integrity of DNA was d...

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Abstract

The invention discloses a micro-satellite primer used for identifying Tridacna maxima and Tridacna rosewateri, and an identification method. The sequence of the micro-satellite primer TC89 is F:5'-ACAAACGAAGCAGGTGTT-3', R:5'- GGTTCTGGATAAATGGGT-3'. The identification method is capable of realizing rapid and accurate identification of Tridacna maxima and Tridacna rosewateri, is simple, accurate, and effective, and is low in cost; the results are visual; no influence is caused by the environment or the growth period.

Description

Technical field: [0001] The invention belongs to the technical field of shellfish molecular markers and identification of hybrid species in aquatic biology technology, and specifically relates to a microsatellite primer and an identification method for identifying giant clams long and giant clams nova. Background technique: [0002] Tridacna is a kind of tropical large marine coral reef benthic shellfish, mainly distributed in the western Pacific Ocean, Indian Ocean and other sea areas. The coral reef lagoons and reefs in the South my country Sea are the traditional natural distribution areas of giant clams. Tridacna has high economic value, wide range of uses, and a large market demand, mainly including food, live aquariums and shell craft decorations. In recent years, due to overfishing, giant clam resources have been severely damaged. For example, giant clam kushii is almost extinct in the South my country Sea, scaleless giant clam is also extremely rare, and most other g...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 马海涛喻子牛张跃环肖述
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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