A kind of identification primer and method suitable for giant clam oyster, common species of giant clam in the South China Sea and its hybrids

Abstract

The invention discloses an identification primer and method suitable for giant clam oysters, common species of giant clam in the South China Sea and hybrids thereof. The identification primers are TC114 primer pair, and its forward and reverse primers are as follows: F: 5'-AACACTGATTGGGCTCTT-3'R: 5'-TTGACGACGATGACGATA-3'. The present invention screens out a pair of safflower clam microsatellite primers from 60 pairs of giant clam microsatellite primers, which can be used in giant clam and South China Sea common species (scaleless giant clam, scaled giant clam, long giant clam, safflower giant clam and nova giant clam) The microsatellite primers that are commonly used in giant clam) and can be distinguished from the common giant clam in the South China Sea according to the size of the amplified bands, and have clear band patterns, good repeatability, and high reliability: TC114 primer pair, and can be used in giant clam and South China Sea Individual identification of hybrid species among common giant clams also has potential application value.

Description

technical field

[0001] The invention belongs to the shellfish molecular marker and species identification technology in the aquatic biology technology, and specifically relates to a method of using microsatellite primers to identify giant clam species and common species of giant clam in the South China Sea (scaleless giant clam, scaled giant clam, long clam, tomato The identification primers and methods of Tridacna red and Tridacna nova) and the potential application value of identifying hybrid individuals between them. Background technique

[0002] Tridacna is a kind of tropical large marine coral reef benthic shellfish. Common giant clam species distributed in the South China Sea mainly include scaleless giant clam, scaly giant clam, long giant clam, saffron giant clam, and nova giant clam. and giant oysters of the genus Giant oyster. Tridacna is a species of high economic value. It is the material basis of tropical marine life and fishery economy, providing resources suc...

Images