Tridacna immune-related saa gene and its application in preparation of giant clam pathology detection reagents

A technology related to immunity and detection kits, applied in the field of molecular biology, can solve problems such as the trough of giant clam research and few research reports, so as to prevent the expansion of disasters, improve the ability of germplasm innovation, and support high efficiency and sustainability The effect of development
CN110669769BActive Publication Date: 2021-06-01SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
4 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Publication Date
2021-06-01

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a giant clam immune-related SAA gene and its application in preparing a giant clam pathological detection reagent. The present invention clones the SAA gene from Tridacna safranata for the first time, and designs general primers TcSAA‑F / TcSAA‑R for the gene in several common Tridacna. Quantitative PCR experiments showed that the SAA gene of Tridacna safranatum was significantly up-regulated after Vibrio infection, which was very similar to that of mammals. In other pathological conditions, the expression of this gene was also significantly up-regulated. Therefore, the SAA gene can also be used as a gold standard for detecting the health status of giant clams. The SAA gene and primers TcSAA-F / TcSAA-R of the present invention can be used to monitor the health status of giant clam, provide a positive and accurate warning signal for the prevention of giant clam disease, and can effectively prevent the expansion of disasters. The application of this technology will further enhance the ability of giant clam breeding germplasm innovation and support the efficient and sustainable development of the industry.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field:

[0001] The invention belongs to the technical field of molecular biology, and in particular relates to a SAA gene related to immunity of giant clams and its application in preparing reagents for detecting the pathology of giant clams. Background technique:

[0002] Tridacna belongs to the phylum Molluscs, Phyllobranchia, Pleurobranches, and Tridacnaidae. There are 2 genera. There are 9 species in the genus Tridacna, and only 1 species in the genus Tridacna. Tridacna is the largest shellfish today, and some species can reach about 1 meter in size and weigh more than 300 kilograms. In addition to being the largest in size, Tridacna can also be self-supporting, because there are symbiotic zooxanthellae in its mantle , can carry out photosynthesis under light conditions, provide nutrients for giant clams, enable them to survive in the open sea where nutrients are extremely deficient, and provide the cornerstone for the construction of coral reefs. Tridacna are ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More