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A chemical method to effectively avoid self-fertilization of Tridacna mussels

A technique of self-fertilization, chemical method, applied in the fields of botanical equipment and methods, preservation of human or animal body, animal repellent, etc.

Active Publication Date: 2018-08-17
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Both allicin and matrine are broad-spectrum bactericidal natural plant-derived drugs, which are not harmful to the body and are environmentally friendly. It has been reported that allicin has a strong killing effect on human and mouse sperm (Qian Yaoxian 1986), but there is a complete blank for the relevant research practices of aquatic invertebrates with huge differences

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 The avoidance and prevention of self-fertilization of giant clam Tridacna maxima:

[0013] At the beginning of 2016, we carried out the research on the artificial breeding technology of giant clam (Tridacna maxima) at the Hainan Sanya Comprehensive Experimental Station of the Chinese Academy of Sciences. The artificial induced spawning method stimulated the production of 4 seedlings. In order to avoid self-fertilization and improve the breeding efficiency of seedlings, the specific operations are as follows:

[0014] 1. Use sand-filtered seawater to make 10mg / ml allicin mother liquor in advance. When using, mix the newly prepared matrine and allicin according to the effective content of 1:10 to make 100ppm allicin and 10ppm matrine The medicated bath liquid is 30L in total, and the sand-filtered seawater is used as a solvent.

[0015] 2. Assign ID numbers to all the induced labor giant clams, place them in the sperm collection bucket (corresponding to the ID n...

Embodiment 2

[0019] Example 2: Avoidance and prevention of self-fertilization of Tridac oyster Hippopus hippopus:

[0020] At the beginning of 2016, four 200L capacity plastic barrels were used to carry out the artificial breeding of giant oysters (Hippopus hippopus). In order to avoid self-fertilization and improve seed breeding efficiency, the specific operations are as follows:

[0021] 1. Use sand filter seawater to make 10mg / ml allicin mother liquor in advance. When using, mix the newly prepared matrine and allicin according to the effective content of 1:10 to make 100ppm allicin and 10ppm matrine The medicated bath liquid is 30L in total, and the medicated bath liquid uses sand-filtered seawater as a solvent.

[0022] 2. Assign ID numbers to all the induced oysters, place them in the sperm collection bucket (corresponding to the ID number), start timing from the start of mass ejaculation, and obtain a certain concentration of high-quality sperm after 2-3 minutes of discharge, and us...

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Abstract

The invention discloses a chemical method for effectively avoiding self-fertilization of tridacna. The method includes: allocating ID numbers to all tridacna subjected to induction, respectively placing into a sperm collection drum, timing from spermination in large quantity, obtaining sperms after spermination for 2-3min, filtering through a 500-mesh bolting silk screen to obtain filtrate which is sperm fluid, and marking +ID numbers ; moving the tridacna from the sperm collection drum into an ovum collection drum containing medicated bath liquid, wherein the liquid level of the medicated bath liquid is at least higher than a highest position of the tridacna; after ovulation for 15-20min, taking out the tridacna to obtain ovum cell liquid, filtering the ovum cell liquid through a 300-mesh bolting silk screen to obtain ovum cells on the bolting silk screen, cleaning the ovum cells on the bolting silk screen with seawater, and marking +ID numbers; checking sperm and ovum gamete quality, abandoning low-quality gametes, and subjecting the sperms and the ova different in source according to the different ID numbers to artificial fertilization as required.

Description

technical field [0001] The invention belongs to the fields of giant clam shellfish breeding and ecological resource restoration in the marine aquatic industry, and in particular relates to a chemical method for effectively avoiding giant clam shellfish self-fertilization. Background technique: [0002] Tridacna shellfish is an important marine economic shellfish. Its meat is sweet and crisp, and it has been reported to have the effect of nourishing yin and nourishing yang. At the same time, its shell can be carved and processed into valuable handicrafts. There used to be a large number of distributions in the South my country Sea. According to data, there are mainly species of giant clams, saffron clams, scaled clams, and oysters in the South my country Sea. At present, the library-type giant clam is nearly extinct in my country, and other species of giant clam are also facing unfavorable situations such as illegal harvesting and digging, as well as changes in the ecological...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/54
CPCA01N41/02A01N43/90A01N2300/00Y02A40/81
Inventor 肖述喻子牛张跃环马海涛向志明
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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