LAMP kit for testing type O foot and mouth disease virus, construction method and application thereof

The technology of a foot-and-mouth disease virus and a kit is applied to the LAMP kit for detecting O-type foot-and-mouth disease virus and its establishment and application field. Simple operation effect

Inactive Publication Date: 2008-10-29
SHANGHAI UNIV
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  • LAMP kit for testing type O foot and mouth disease virus, construction method and application thereof
  • LAMP kit for testing type O foot and mouth disease virus, construction method and application thereof
  • LAMP kit for testing type O foot and mouth disease virus, construction method and application thereof

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Embodiment Construction

[0033] 1. Specific DNA sequence search

[0034] The genome sequences of several strains of foot-and-mouth disease virus type O were retrieved from the American gene database-GenBank, and the homology analysis was carried out by BLAST software to find a specific conserved target sequence for LAMP primer design. The specific conserved target sequence is:

[0035] CCT TGA GGC TAT CCT CTC CTT TGC ACG CCG TGG GAC CAT ACA GGA GAA GTT GATCTC CGT GGC AGG ACT CGC CGT CCA CTC TGG ACC AGA CGA GTA CCG GCG TCT CTT TGAGCC TTT CCA AGG TCT CTT TGA GAT TCC AAG CTA CAG ATC ACT TTA CCT GCG TTG GGTGAA CGC CGT GTG CGG TGA CGC ATA ATC CCT CAG

[0036] 2. Design of LAMP primers

[0037] Design primers by special LAMP primer design software

[0038] Primer design V3 (http: / / primerexplorer.jp / elamp3.0.0 / index.html)

[0039] The specific LAMP primers designed are:

[0040]

[0041] 3. Establishment of detection system

[0042] Set different final concentrations of Mg 2+ (2, 4, 6, 8, 10, 12mM),...

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Abstract

The invention relates to a LAMP reagent kit used for detecting O type foot-and-mouth disease virus, and an establishing method and the application thereof. The reagent kit is a detection system composed of LAMP reaction solution including six LAMP primers; the LAMP reaction solution of 23 microlitres has the concrete collocations just as the rightward presentation; the detection system can detect the O type foot-and-mouth disease virus rapidly, conveniently and efficiently under the isothermal condition in a high specific and high sensitive manner without complicated instruments; therefore, a new technology platform is provided for food safety inspection, and the urgent need for the field detection on the foot-and-mouth disease at present is better satisfied; the LAMP reagent is used in the field detection of import and export quarantines, food sanitation departments, animal husbandry farms, etc., is easy for large-scale popularization and application, and has wide market prospect, and bigger economic benefits as well as social benefits.

Description

technical field [0001] The invention relates to a kit for detecting O-type foot-and-mouth disease virus and its establishment method and application, in particular to a LAMP kit for detecting O-type foot-and-mouth disease virus and its establishment method and application. technical background [0002] The foot-and-mouth disease virus belongs to the genus B virus of the picornaviridae family. It is the most subtle level of viruses known so far. The foot-and-mouth disease caused by it is listed as the first class A animal infectious disease by the World Organization for Animal Health. Its main hazards are Pigs, sheep, cattle and other cloven-hoofed animals have a morbidity rate of up to 100%, and can form a large-scale epidemic, causing serious harm. They are mandatory inspection items for entry-exit quarantine and compulsory reporting by the state. However, the current detection method is generally to first sample on-site, and then send it to the laboratory for detection, wh...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68
Inventor 陈沁方雪恩
Owner SHANGHAI UNIV
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