SNP marker related to sugar tolerance of grass carps and application of SNP marker

A grass carp and marking technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve problems such as rising feed costs

Active Publication Date: 2016-04-20
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, grass carp farming still has a certain benefit space, but in terms of production expenditure, feed costs have been rising since 2008, and feed costs account for more than 70% of the cost of grass carp farming

Method used

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  • SNP marker related to sugar tolerance of grass carps and application of SNP marker
  • SNP marker related to sugar tolerance of grass carps and application of SNP marker
  • SNP marker related to sugar tolerance of grass carps and application of SNP marker

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Experimental program
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Effect test

Embodiment 1

[0029] 1 Feed preparation

[0030] Purchase imported fish meal, soybean meal, distiller’s grains, flour, soybean oil, calcium dihydrogen phosphate, choline chloride, salt, premix (multi-dimensional, multi-mineral), and mix these feed ingredients at the Pearl River Fisheries Research Institute feed factory after mixing them evenly. Stir a proper amount of water evenly, use a granulator to make two kinds of granules with a particle size of about 5mm, put the prepared pellets into an oven, dry at a constant temperature of 56°C for 24h, and store them at -20°C for later use. The dried feed was sent to Guangzhou Analysis and Testing Center to test its main components. The analysis results are shown in Table 1. The sugar content of these two feeds is 39.4g / 100g and 45.4g / 100g, respectively, which are recorded as ordinary feed and high-sugar feed. .

[0031] Table 1 Composition of experimental feed

[0032]

[0033] 2 Experimental fish farming

[0034] From the existing Pearl River Fisheri...

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Abstract

The invention provides an SNP marker related to the sugar tolerance of grass carps and application of the SNP marker. The SNP marker comprises two SNP loci, namely H3 and H4, wherein H3 is located at position 1817 from the 5' end of the nucleotide sequence shown as SEQ ID NO:1 in the specification, and base at the position 1817 is T or C; H4 is located at position 1818 from the 5' end of the nucleotide sequence shown as SEQ ID NO:1 in the specification, and base at the position 1818 is G or T. The SNP marker provided by the invention is closely related to the sugar tolerance of the grass carps, and can be effectively used for identification or selective breeding of high-sugar-tolerance grass carp species.

Description

【Technical Field】 [0001] The invention relates to a SNP marker and its application, in particular to a SNP marker related to the sugar tolerance of grass carp and its application, and belongs to the field of molecular markers. 【Background technique】 [0002] Grass carp (Ctenopharyngodonidella) belongs to the subfamily Leuciscinae (Leuciscinae) and the genus Grass carp (Ctenopharyngodon). It is the largest annual output of freshwater farmed fish in my country. Grass carp is loved by people because of its fast growth rate and delicious meat. At present, there is still some room for benefit in the cultivation of grass carp, but from the perspective of production expenditure, the cost of feed has been rising since 2008, and the cost of feed has accounted for more than 70% of the cost of grass carp culture. Because sugar is the cheapest energy supply among the three major nutrients, sugar content is often increased in production to reduce feed costs. Although grass carp is a herbivoro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2521/301
Inventor 樊佳佳白俊杰朱冰全迎春姜鹏李胜杰马冬梅
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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