Primer composition for identification of human adenovirus type 55 and application thereof

A primer composition and adenovirus technology, applied in the direction of recombinant DNA technology, microbial measurement/testing, biochemical equipment and methods, etc., can solve the problems that take a long time and are difficult to meet the needs of rapid diagnosis of outbreaks, and achieve High sensitivity, high specificity, convenient and simple identification
CN104278024AActive Publication Date: 2015-01-14MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
Publication Date
2015-01-14

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Abstract

The invention discloses a primer composition for identification of human adenovirus type 55 and application thereof. The primer composition provided by the invention includes a DNA fragment-I, II, a DNA fragment-II, a DNA fragment-III and a DNA fragment-IV, all the fragments are single-stranded DNA fragments and are shown as sequence 1, sequence 2, sequence 3 and sequence 4 in order. The primer composition can also include a single-stranded DNA fragment-V and / or a single-stranded DNA fragment-VI, and the fragments are shown as sequence 5 and sequence 6 in order. The primer composition provided by the invention is applicable to specific detection and quantitative analysis on the human adenovirus type 55. The primer composition provided by the invention has the advantages of: (1) amplification reaction just under constant temperature and no need for special equipment; (2) high specificity; (3) fast speed and high efficiency, completion of the amplification reaction in a short period of time; (4) high sensitivity; and (5) convenient and simple identification. The primer composition provided by the invention is especially suitable for rapid detection of viruses in clinical samples.
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Description

technical field

[0001] The invention relates to a primer composition for identifying human adenovirus type 55 and its application. Background technique

[0002] Human Adenovirus (HAdV) is a group of non-enveloped double-stranded DNA viruses, which are divided into 1-55 serotypes and divided into 6 serological groups A-F. Human adenovirus spreads through the respiratory tract and digestive tract, causing a variety of infections and causing serious harm to children and immunocompromised persons. The possible diseases caused by adenovirus infection include: acute adenovirus pneumonia, acute gastroenteritis, keratitis, celiac disease, and acute interstitial nephritis. The positive rate of adenovirus infection in children with acute lower respiratory tract infection can be as high as 4.1%. In North America, Europe and Asia in recent decades, human adenovirus group B infection is considered to be an important factor leading to acute respiratory infection (ARD).

[0003] HAdV11 ...

Claims

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