MiR-3197 molecular marker for detecting type 2 diabetic retinopathy and amplification primer and application thereof

A technology for mir-3197 and retinopathy, which is applied in biochemical equipment and methods, microbe measurement/inspection, DNA/RNA fragments, etc., can solve the problems of undisclosed detection of type 2 diabetic retinopathy, etc., to achieve strong pertinence, Rapid identification and high detection efficiency

Active Publication Date: 2016-04-13
NINGBO UNIV
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Problems solved by technology

However, at present, there is no published research report on the detection of type 2 diabetic retinopathy using miRNA molecules as molecular markers for the prediction or diagnosis of type 2 diabetic retinopathy.

Method used

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  • MiR-3197 molecular marker for detecting type 2 diabetic retinopathy and amplification primer and application thereof
  • MiR-3197 molecular marker for detecting type 2 diabetic retinopathy and amplification primer and application thereof
  • MiR-3197 molecular marker for detecting type 2 diabetic retinopathy and amplification primer and application thereof

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[0017] 1. Collection of research objects

[0018] All samples in this study were collected with the approval of the medical research ethics committee of the unit and the informed consent of the patients, including 144 serum samples of the healthy group, 70 serum samples of the prediabetes group, 117 serum samples of T2DM and 75 serum samples of DR. Collection requirements: ① Diagnosis was confirmed by fundus fluorescein angiography. DR was confirmed to have microangioma, hemorrhage, hard exudation, cotton-like soft exudation and other symptoms; healthy group, pre-diabetic group, and T2DM group were confirmed to have mydriasis in both eyes Fundus examination was normal. All excluded ①acute and chronic infectious diseases; ②severe heart, brain, liver function damage and other endocrine diseases affecting glucose metabolism; ③obviously turbid refractive media in one or both eyes, affecting fundus observers.

[0019] 2. Use the gene chip to screen out the serum samples of 5 cases...

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Abstract

The invention discloses a miR-3197 molecular marker for detecting type 2 diabetic retinopathy and an amplification primer and an application thereof. The miR-3197 molecular marker is characterized in that a nucleotide sequence of the miR-3197 molecular marker is 5'GGAGGCGCAGGCUCGGAAAGGCG-3', and a nucleotide sequence of a positive amplification primer of the miR-3197 molecular marker is 5'-GGA GGC GCA GGC TCG GAA -3'. The miR-3197 molecular marker has the advantages of high detection efficiency and high specificity, and can be applied to auxiliary diagnosis, detection or medicament screening of the type 2 diabetic retinopathy.

Description

technical field [0001] The invention relates to a miRNA molecular marker, in particular to a miR-3197 molecular marker for detecting type 2 diabetic retinopathy, an amplification primer and application thereof. Background technique [0002] Type 2 diabetes (Type2diabetesmellitus, T2DM), also known as non-insulin-dependent diabetes, is the most important type of diabetes, accounting for about 90% (23499527). It is a group of multifactorial metabolic syndromes characterized by chronic hyperglycemia, which is one of the major causes of death, disability and cost in the world. Diabetic retinopathy (Diabetic retinopathy, DR), as one of the most serious microvascular complications of diabetes, has become the first cause of blindness in diabetic patients. Currently, there are about 93 million DR patients worldwide (22,301,125). There are 92.4 million diabetic patients in my country, ranking first in the world, and the prevalence of DR is 37% (20335585). And with the increase in t...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 韩丽媛陆南佳刘艳芬段东辉汪凯悦顾凯波张璐段世伟
Owner NINGBO UNIV
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