Universal primers for cephalopod mitochondrial COI genes and design and amplification method thereof

A universal primer and cephalopod technology, which is applied in biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems of inability to identify cephalopod mitochondrial COI gene, time-consuming, inconvenient and efficient, etc. , achieve the effect of cost saving and simple operation
CN107937560AInactive Publication Date: 2018-04-20ZHEJIANG OCEAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG OCEAN UNIV
Publication Date
2018-04-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to universal primers capable of highly efficiently and quickly amplifying cephalopod mitochondrial COI genes of various cephalopods, and a design and amplification method thereof. By designing the universal primers of the cephalopod mitochondrial COI genes, various cephalopod COI genes can be amplified at a time in batches, specific primers are not needed to be designed for acertain single species, the time cost is saved, the work efficiency is greatly improved, and the method has high-efficiency and fast characteristics and provides important molecular means and data accumulation for species identification, systematic evolution, germplasm resource conservation and sustainable development and utilization of the cephalopods.
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Description

technical field

[0001] The invention relates to the field of cephalopod mitochondrial genome research, in particular to a general primer capable of efficiently and quickly amplifying cephalopod mitochondrial COI genes of various cephalopods and a method for designing and amplifying them. Background technique

[0002] Cephalopods are an important group of molluscs. They are widely distributed in the three oceans of the world and the Antarctic waters. They not only have high economic value, but also occupy an important position in marine ecology. There are 2 subclasses, 11 orders, 50 families, and 154 genera and about 718 species of cephalopods. Since the 1970s, due to the general decline of traditional economic species resources in the world, the cephalopod resources at the low end of the food chain have attracted attention from countries all over the world. regional attention. According to the statistics of FAO, since 2000, the output of cephalopods in the world is about 31...

Claims

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