Closed SARS-CoV-2 isothermal amplification nucleic acid detection kit

Abstract

The invention relates to the technical field of virus nucleic acid detection methods, and particularly relates to an RT-LAMP nucleic acid detection primer group of SARS-CoV-2 and a closed SARS-CoV-2 isothermal amplification nucleic acid detection kit. According to the kit provided by the invention, nucleic acid extraction is not needed for virus RNA of a throat swab sample, and the sample can be directly amplified. The kit is a visual instant screening kit for the SARS-CoV-2 by a one-step method. The kit has good specificity, can eliminate non-specific interference of other coronavirus strains, is quick and efficient, and can complete detection within one hour. Large expensive and precise instruments and equipment are not needed, only a common metal bath or water bath kettle is needed, detection cost is low, toxic reagents are not involved and closed operation is performed in the whole process, secondary pollution is avoided, and safety of operators and an environment is ensured.

Description

technical field [0001] The invention relates to the technical field of viral nucleic acid detection methods, in particular to a SARS-CoV-2 RT-LAMP nucleic acid detection primer set and a closed SARS-CoV-2 isothermal amplification nucleic acid detection kit. Background technique [0002] With the release of the "Diagnosis and Treatment Program for Pneumonia Infected by Novel Coronavirus (Trial Sixth Edition)", the importance of nucleic acid testing as an important standard for diagnosis has been generally recognized. However, nucleic acid detection involves extraction, amplification, detection, etc., and has high requirements for the laboratory environment and operators. A complete nucleic acid detection procedure for the new coronavirus often requires professional testers to operate with their hands off the platform for more than 3 hours in a row, involving large-scale Therefore, the popularization and on-site application of nucleic acid detection are limited, which leads to...

AI Technical Summary

Problems solved by technology

[0003] (1) More than 90% of them are in the form of nucleic acid diagnostic kits, which need to be extracted, amplified and tested. The operation is cumbersome and requires professionals and complex P2 laboratories. It is difficult to realize the on-site detection of new coronavirus pneumonia (COVID-19) Real-time detection;

[0004] (2) Most of the qPCR methods are used, and the detection time is relatively long (about 4 hours) due to the heating and cooling cycles involved, which is not conducive to rapid on-site detection;

However, the isothermal amplification kits developed by the above companies have not yet achieved the step of exempting nucleic acid extraction.

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