Detection method of molecules, and appliction

A molecular and sample technology, applied in new detection fields, can solve the problems of cumbersome methods, unusable detection, low sensitivity, etc., and achieve the effect of good repeatability, less sample consumption and high sensitivity

Inactive Publication Date: 2010-04-07
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, this method is cumbersome, has low sensitivity, and the minimum detection amount is 10 ng / ml, which is limited to blood testing and cannot be used for the detection of samples such as cerebrospinal fluid.

Method used

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  • Detection method of molecules, and appliction
  • Detection method of molecules, and appliction
  • Detection method of molecules, and appliction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Detection of sCD146 by label-free detection technology optical ellipsometry imaging system

[0034] In view of the fact that the lowest detection value of sCD146 in the commercial kit is 10 ng / ml, it cannot be used for the detection of sCD146 in samples such as cerebrospinal fluid and arthritis synovial fluid. Therefore, we employ protein-optical chips to address this issue. It is characterized in that the purified anti-CD146 monoclonal antibody AA98 (see Chinese Invention Patent Application "New Antibody for Efficient and Broad-spectrum Inhibition of Tumor Growth and Metastasis", Patent No. 99107586.2; and references (Yan, Lin et al.Blood, 2003 )) fixed on the activated silicon chip (24 channels), the sample to be tested flows over the chip surface immobilized with antibodies to fully react the antigen and antibody, the specific reaction signal of the antigen and antibody is detected by the ellipsometry system, and the software is used to analyze and shoot The graysca...

Embodiment 2

[0054] The Clinical Significance of Detecting the Expression of CD146 in Peripheral Blood Leukocyte Membrane of Patients

[0055] 1. Sample source and clinical data: All patients were inpatients in the Department of Internal Medicine of Peking Union Medical College Hospital from April 2004 to April 2005 with complete clinical data. Among them, there were 58 cases of systemic vasculitis, 31 cases of males and 27 cases of females; 15 cases of patients with systemic lupus erythematosus (SLE). Takayasu arteritis (TA) in 11 cases, Wegener's granulomatosis (WG) in 16 cases, microscopic polyangiitis (MPA) in 19 cases, allergic granulomatous angiitis (CSS) in 6 cases. TA, PAN, WG, MPA, and CSS patients all met the classification criteria of systemic vasculitis of the American College of Rheumatology (ACR) in 1990; systemic lupus erythematosus met the classification criteria of the 1982 ACR. Blood samples were collected from all patients when they were diagnosed or admitted to the hos...

Embodiment 3

[0074] ELISA detection of sCD146 content in serum of patients with autoimmune diseases

[0075] 1. Sample source and clinical data: A total of 67 patients were inpatients in the Department of Internal Medicine of Peking Union Medical College Hospital from April 2004 to April 2005 with complete clinical data. All patients were diagnosed in accordance with the 1990 American College of Rheumatology (ACR) classification criteria for systemic vasculitis. Patients with systemic lupus erythematosus (SLE) met the classification criteria of the 1982 ACR. The details of the patients are shown in Table 3. 12 of them included 2 cases of BD, 4 cases of CSS, 4 cases of MPA, 1 case of SLE, and 1 case of WG. The patients were detected at two time points when they were clearly diagnosed and in the active stage and after treatment improved.

[0076] 2. Specific implementation steps:

[0077] 1) Collection of serum: 2 ml of blood was drawn on an empty stomach in the morning, centrifuged after...

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Abstract

A method for detecting CD146 molecule includes utilizing protein chip and optical oval polarization microscope imaging technique to prepare CD146 antibody optical chip being used to detect CD146 content in body liquid of patient, utilizing stream cell technique to detect CD146 presentation state on white blood cell of patient.

Description

technical field [0001] The invention relates to the field of molecular detection, more specifically, the invention relates to a new detection method and application of the vascular endothelial adhesion molecule CD146. Background technique [0002] Vascular endothelial cells are a layer of cells that continuously coat the intima of blood vessels throughout the body. It is not only a barrier between blood and tissue, but also has multiple functions such as controlling vascular permeability, regulating the material exchange between tissue and blood, and inhibiting the migration of blood vessel wall cells. Changes or damage to the structure and function of vascular endothelial cells cause many important diseases. Vascular endothelial cells are also the earliest target cells to be affected in tumors, infections, autoimmune diseases, cardiovascular diseases and transplant immune rejection. Changes in vascular endothelial cells not only increase vascular permeability, but also al...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/551G01N21/17G01N33/577
Inventor 阎锡蕴张佰茹张莹靳刚
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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