A closed sars-cov-2 isothermal amplification nucleic acid detection kit

Abstract

The invention relates to the technical field of viral nucleic acid detection methods, specifically a SARS-CoV-2 RT-LAMP nucleic acid detection primer set and a closed SARS-CoV-2 isothermal amplification nucleic acid detection kit. The kit of the present invention does not need nucleic acid extraction for virus RNA of throat swab samples, and can directly amplify the samples. It is a one-step visualized real-time screening kit for SARS-CoV-2, which has good specificity and can exclude other The non-specific interference of coronavirus strains is fast and efficient, and the detection can be completed within 1 hour, and does not require large, expensive and sophisticated instruments and equipment, only ordinary metal baths or water baths are needed, the detection cost is low, and the whole process does not involve toxic reagents. Closed operation avoids secondary pollution and ensures the safety of operators and the environment.

Description

Technical field [0001] The present invention relates to the technical findings of viral nucleic acid detection methods, and more particularly to an RT-LAMP nucleic acid detection primer group of SARS-COV-2, and a closed SARS-COV-2 isothermal expansion nucleic acid detection kit. Background technique [0002] With the release of "new coronavirus infected pneumonia diagnosis, nucleic acid detection as an important criterion for diagnosis, its importance is generally recognized. However, nucleic acid detection involves extraction, amplification, detection, etc., which requires high requirements for laboratory environment and operators, and a complete new crown virus nucleic acid detection process often requires professional testers to pass two consecutive hands, involving large-scale Fluorescence quantitative PCR instrument, so the popularity and field application of nucleic acid detection are limited, thereby causing a large number of patients with clinical symptoms that cannot be ...

AI Technical Summary

Problems solved by technology

[0003] (1) More than 90% of them are in the form of nucleic acid diagnostic kits, which need to be extracted, amplified and tested. The operation is cumbersome and requires professionals and complex P2 laboratories. It is difficult to realize the on-site detection of new coronavirus pneumonia (COVID-19) Real-time detection;

[0004] (2) Most of the qPCR methods are used, and the detection time is relatively long (about 4 hours) due to the heating and cooling cycles involved, which is not conducive to rapid on-site detection;

However, the isothermal amplification kits developed by the above companies have not yet achieved the step of exempting nucleic acid extraction.

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