Molecular marker MAG7237 of wheat fusarium head blight infection resistant gene Fhb4 and application of molecular marker MAG7237
A technology of MAG7237 and MAG7237-F, which is applied in the field of molecular markers of wheat head blight resistance gene Fhb4, can solve the problems of large genetic distance and speed up gene cloning research, achieve stable amplification, convenient identification, and improve selection and identification efficiency effect
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[0044] The molecular marker of wheat head blight resistance gene Fhb4 was obtained by the following methods:
[0045] (1) Wangshuibai Fhb4 near-isogenic line NMAS007 and its recurrent parent Mianyang 99-323F 2:3 Creation of populations and screening of Fhb4 segment recombinants:
[0046] (1) NMAS007 (♀) and wheat variety Mianyang 99-323 hybrid F 1 , F 1 Selfing produced F containing 4303 individuals 2 group;
[0047] (2) Using the border markers Xhbg226 and Xgwm149 of Fhb4 (Torada et al.2006; et al.1998) in F 2 In the population, 187 heterozygous individuals with recombination in this segment were screened, and the same marker was used in its F 3 Generation screening yielded 459 homozygous individuals recombined in this segment.
[0048] (2) Identification of recombinant disease-resistant phenotypes
[0049] Sow susceptible wheat grains in the field about ten days before the recombinants flower to inoculate these homozygous recombinants, and repeat once a week late...
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