Streptomyces venezuelae, and authentication method and application thereof

A technology of Streptomyces venezuela and Streptomyces, applied in the field of microorganisms, to achieve the effect of luxuriant and well-developed air filaments

Active Publication Date: 2020-02-07
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the current problem is that in order to quickly and accurately obtain the characteristic information of Venezuelan zipper mold in the soil above the oil and gas fields, it is urgent to develop research on the acquisition of typical strains and the detection technology in the mixed system

Method used

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  • Streptomyces venezuelae, and authentication method and application thereof
  • Streptomyces venezuelae, and authentication method and application thereof
  • Streptomyces venezuelae, and authentication method and application thereof

Examples

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Embodiment 1

[0090] Example 1: Utilize Streptomyces venezuela SINOPEC22 to identify soil samples above Chunguang Oilfield.

[0091] (1) According to the 16S rDNA conserved sequence of Streptomyces venezuela SINOPEC22, four sets of 16S rDNA-specific primer pairs were designed. The specific sequences are:

[0092] Primer pair 1: F1:5'-GCTTAATTCGACGCAACGCGAAGAACCT-3',

[0093] R1: 5'-GCAATGCTGATCTGCGATTACTAGCAACTCC-3';

[0094] Primer pair 2: F2: 5'-CGGGTTGTAAACCTCTTTCAGCAGGAGGAA-3',

[0095] R2: 5'-ATCGCAGCTCTTTGTACCGGCCATTGTA-3';

[0096] Primer pair 3: F3: 5'-CGGGTTGTAAACCTCTTTCAGCAGGAGGAA-3',

[0097] R3: 5'-GCAATGCTGATCTGCGATTACTAGCAACTCC-3';

[0098] Primer pair 4: F4: 5'-GCAATCTGCCCTTCACTCTGGGACAAGC-3',

[0099] R4: 5'-TGATCTGCGATTACTAGCAACTCCGACTTCAT-3'.

[0100] The obtained target band sizes were: primer pair 1: 420bp, primer pair 2: 829bp; primer pair 3: 1036bp; primer pair 4: 1223bp.

[0101] (2) Extract the whole genome of microorganisms in the soil.

[0102] Weigh 0.5g o...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to streptomyces venezuelae, and an authentication method and application thereof. The invention screens an oiland gas indication microorganism, i.e., the streptomyces venezuelae, with high specificity, the streptomyces venezuelae in soil can be quickly detected through a specific primer, a defect that an authentication period is short and an applicable range is small in the common art is eliminated so as to be quick and convenient, and the concentration anomaly characteristics of the microorganism are analyzed to predict an oil and gas enrichment region and an oil and gas reservoir. The invention also provides a method for indicating the concentration of upward floating gaseous hydrocarbon of the oiland gas reservoir by the streptomyces venezuelae. The method adopts an improved primer to amplify the 16S rDNA conserved sequence of the streptomyces venezuelae, and according to the luminance of a target stripe in an amplification product, the concentration of the floating gaseous hydrocarbon can be accurately and efficiently judged.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a Streptomyces venezuela and its identification method and application. Background technique [0002] Microbial oil and gas exploration technology is a surface exploration technology derived from the principle of light hydrocarbon micro-leakage in oil and gas reservoirs. The light hydrocarbon part (C1-C5) in the oil and gas reservoir passes through the overlying sedimentary layer in the form of micro-leakage, and the gaseous hydrocarbon undergoes a long migration process, which makes the hydrocarbon content in the near-surface soil or water layer reach a higher level. concentration. This high concentration of gaseous hydrocarbons can also be utilized by microorganisms. The reproduction and growth of microorganisms specialized in utilizing light hydrocarbons are induced in the near-surface soil, and the types and concentrations of microorganisms in oil and gas areas are differe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/689C12Q1/64C12R1/61
CPCC12N1/20C12Q1/689C12N1/205C12R2001/61
Inventor 顾磊汤玉平许科伟杨帆高俊阳
Owner CHINA PETROLEUM & CHEM CORP
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